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[191382_at] glutathione S-transferase

Description F35E8.8 /REP_DB=WormBase Gene ID /WP=CE15958 /TR=O45451 /GB=CAB04293.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Glutathione S-transferases.
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 02      4grp_highest_8_8 > 20      4grp_high_8_8 > 31      6grp_highest_nonorm > 50      6grp_total > 34      4grp_total > 32      6grp_highest_sub > De      6grp_cgc > 21      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 364
1 2 3 4 range
A 874.6
(-3)
P 		881.3
(-2.8)
P 		1103.5
(3)
P 		1056.9
(1.8)
P 		229
B 868.4
(-3)
P 		876.3
(-2.9)
P 		1232.2
(3)
P 		1082.8
(0.5)
P 		364
C 887.7
(-3)
P 		973.4
(0)
P 		1057.6
(3)
P 		1039.9
(2.4)
P 		170
avr 876.9
(-3) 		910.3
(-2.2) 		1131.1
(3) 		1059.9
(1.3) 		254

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 434
1 2 3 4 5 6 range
A 635.3
(3)
P 		423
(-0.6)
P 		540.8
(1.4)
P 		285.7
(-3)
P 		290.1
(-2.9)
P 		299
(-2.8)
P 		350
B 672.5
(3)
P 		451.9
(-1)
P 		619.5
(2)
P 		347.4
(-2.9)
P 		342.4
(-3)
P 		433
(-1.4)
P 		330
C 568.5
(3)
P 		545.8
(2.6)
P 		409.2
(0.1)
P 		383.7
(-0.4)
P 		238.9
(-3)
P 		380.5
(-0.4)
P 		330
avr 625.4
(3) 		473.6
(0.3) 		523.2
(1.2) 		338.9
(-2.1) 		290.5
(-3) 		370.8
(-1.6) 		335
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 214
1 2 3 4 range
A 318.5
(3)
P 		191.3
(-1.7)
P 		288.5
(1.9)
P 		154.7
(-3)
P 		164
B 276.9
(2.3)
P 		216.4
(-3)
P 		285.5
(3)
P 		230.7
(-1.8)
P 		69
C 293.9
(0.7)
P 		279.9
(0.2)
P 		368.6
(3)
P 		175.3
(-3)
P 		193
avr 296.4
(2.2) 		229.2
(-1) 		314.2
(3) 		186.9
(-3) 		127

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 162
1 2 3 4 5 6 range
A 117.1
(0.4)
P 		150.4
(3)
P 		117.8
(0.5)
P 		72.5
(-3)
P 		87.5
(-1.8)
P 		72.8
(-3)
P 		78
B 234
(3)
P 		150.4
(-0.5)
P 		150.4
(-0.5)
P 		92
(-3)
P 		139.1
(-1)
P 		161.5
(-0.1)
P 		142
C 210.7
(3)
P 		166.5
(1)
P 		104.1
(-1.8)
P 		77.2
(-3)
P 		77.3
(-3)
P 		127.4
(-0.7)
P 		134
avr 187.3
(3) 		155.8
(1.2) 		124.1
(-0.6) 		80.6
(-3) 		101.3
(-1.8) 		120.6
(-0.8) 		107
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191382_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13766
Sequence Type Exemplar sequence
Representative Public ID F35E8.8 NCBI
Target Description F35E8.8 /REP_DB=WormBase Gene ID /WP=CE15958 /TR=O45451 /GB=CAB04293.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Glutathione S-transferases.
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074582(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074582 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-38) (gst-38) mRNA, complete cds. 11/11 None
SNAP00000019514 ENSEMBL cdna:SNAP chromosome:CEL140:V:15917834:15918520:-1 11/11 None
GENEFINDER00000019522 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:15917834:15918520:-1 11/11 None
F35E8.8 ENSEMBL cdna:known chromosome:CEL140:V:15917834:15918520:-1 gene:F35E8.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:15917833-15918520(-) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-38
Ensembl F35E8.8
Entrez Gene 185299 Entrez gene
SwissProt O45451 EMBL-EBI
WormBase CE15958 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04293 Hypothetical protein F35E8.8 [Caenorhabditis elegans] ref|NP_506983.1| Glutathione S-Transferase family member (gst-38) [Caenorhabditis elegans] 1.0E-119
blast CAE74959 Hypothetical protein CBG22850 [Caenorhabditis briggsae] 8.0E-95
blast CAA86859 Hypothetical protein R03D7.6 [Caenorhabditis elegans] ref|NP_496357.1| Glutathione S-Transferase family member (gst-5) [Caenorhabditis elegans] sp|Q09596|GST5_CAEEL Probable glutathione S-transferase 5 (GST class-sigma) pdb|1ZL9|B Chain B, Crystal Structure Of A Major Nematode C.Elegans Specific Gst (Ce01613) pdb|1ZL9|A Chain A, Crystal Structure Of A Major Nematode C.Elegans Specific Gst (Ce01613) 6.0E-75
Protein Domains
Database ID Description E-Value
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 2.1E-16
Sequence
Target Sequence 
>C. ELEGANS:191382_AT
caagcttacctacttcgatggacgcggagccggagagctctgccgtcaaatctttgctgc
cgccgagcagaaatatgaagataacagacttaccgatgaggagtgggagaagttcaaagc
ggccggaaaaaccccatacaaccagcttccaatgctcgaggtagatggcaaaccactcgc
tcagtcccacgcgatggctcgttatcttgctcgggaattcgggttcaacggaaagagcag
atgggaagaagctcaagtcaactccttggccgaccagtacaaagactattacgcggaggc
tcgtccatacctcgctgtgaagcttggttacacagaaggagacgcggaggctctttacac
aagcgtctatcttccagttttcaagaaacactatggattctttgtcaatgctttgaaggc
cagcgggtcaggattcttggttggaaattccttgacttttattgatttgcttgttgctca
gcattcagctgatttgctgggacgtgaaaagtcggatcttttcaatgatgtcccagagat
gaaggcacattccgaaaaagttcagtcaattcctcagatcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAGCTTACCTACTTCGATGGACGC 214 185 24 Antisense
AGAGCTCTGCCGTCAAATCTTTGCT 183 69 57 Antisense
AATCTTTGCTGCCGCCGAGCAGAAA 260 169 72 Antisense
ACAACCAGCTTCCAATGCTCGAGGT 144 111 161 Antisense
GAGGTAGATGGCAAACCACTCGCTC 410 403 181 Antisense
CTCGTTATCTTGCTCGGGAATTCGG 74 229 221 Antisense
AGAAGCTCAAGTCAACTCCTTGGCC 162 73 270 Antisense
GTCCATACCTCGCTGTGAAGCTTGG 235 477 326 Antisense
ACACAAGCGTCTATCTTCCAGTTTT 576 107 380 Antisense
AAGGCCAGCGGGTCAGGATTCTTGG 501 157 439 Antisense
AAGTTCAGTCAATTCCTCAGATCAA 584 155 581 Antisense
Sequence Source Affymetrix Proprietary Database