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[191362_at] 7TM chemoreceptor, srh family

Description T22F3.5 /REP_DB=WormBase Gene ID /WP=CE13970 /GEN=srh-213 /TR=Q94302 /GB=AAB18308.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster 6grp_total > 65      4grp_total > 57      6grp_cgc > 46      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 37
(0.4)
A 		50.2
(3)
A 		36.1
(0.3)
A 		19.2
(-3)
A 		31
B 1.6
(-3)
A 		17.7
(0)
A 		33.6
(3)
A 		20.9
(0.6)
A 		32
C 29.5
(3)
A 		16.2
(-2.9)
A 		16
(-3)
A 		25.7
(1.3)
A 		14
avr 22.7
(-2.3) 		28
(2.5) 		28.6
(3) 		21.9
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 12.4
(-1.8)
A 		3.4
(-3)
A 		46.9
(3)
A 		30.8
(0.8)
A 		46.8
(3)
A 		25.9
(0.1)
A 		44
B 28.5
(3)
A 		3.9
(-2.6)
A 		2.4
(-3)
A 		8.4
(-1.6)
A 		2.2
(-3)
A 		22
(1.5)
A 		26
C 28.3
(1.2)
A 		39.5
(3)
A 		24.9
(0.7)
A 		29
(1.3)
A 		2.1
(-3)
A 		32.3
(1.8)
A 		37
avr 23.1
(1.1) 		15.6
(-3) 		24.7
(1.9) 		22.7
(0.8) 		17
(-2.2) 		26.7
(3) 		11
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 13.5
(3)
A 		10.9
(1.5)
A 		9.4
(0.7)
A 		2.8
(-3)
A 		11
B 0.5
(-3)
A 		4.4
(0.2)
A 		7.8
(3)
A 		4.4
(0.2)
A 		7
C 9.8
(3)
A 		4.7
(-2.6)
A 		5.6
(-1.6)
A 		4.3
(-3)
A 		6
avr 7.9
(3) 		6.7
(1.2) 		7.6
(2.6) 		3.8
(-3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 2.3
(-2.5)
A 		1.2
(-3)
A 		10.2
(1.2)
A 		7.8
(0.1)
A 		14.1
(3)
A 		6.3
(-0.6)
A 		13
B 9.9
(3)
A 		1.3
(-2.5)
A 		0.6
(-3)
A 		2.2
(-2)
A 		0.9
(-2.8)
A 		8.2
(1.9)
A 		9
C 10.5
(2.2)
A 		12.1
(3)
A 		6.3
(-0.1)
A 		5.8
(-0.3)
A 		0.7
(-3)
A 		10.8
(2.3)
A 		11
avr 7.6
(1.6) 		4.9
(-3) 		5.7
(-1.6) 		5.3
(-2.3) 		5.2
(-2.5) 		8.4
(3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191362_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15182
Sequence Type Exemplar sequence
Representative Public ID T22F3.5 NCBI
Target Description T22F3.5 /REP_DB=WormBase Gene ID /WP=CE13970 /GEN=srh-213 /TR=Q94302 /GB=AAB18308.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071609(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071609 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-213) (srh-213) mRNA, complete cds. 11/11 None
SNAP00000034227 ENSEMBL cdna:SNAP chromosome:CEL140:V:3592583:3594788:-1 11/11 None
T22F3.5 ENSEMBL cdna:known chromosome:CEL140:V:3592583:3594788:-1 gene:T22F3.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3592582-3594788(-) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, srh family
Gene Symbol srh-213
Ensembl T22F3.5
Entrez Gene 188749 Entrez gene
SwissProt Q94302 EMBL-EBI
WormBase CE13970 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB18308 Serpentine receptor, class h protein 213 [Caenorhabditis elegans] ref|NP_504010.1| Serpentine Receptor, class H family member (srh-213) [Caenorhabditis elegans] 1.0E-179
blast AAB18309 Serpentine receptor, class h protein 212 [Caenorhabditis elegans] ref|NP_504011.1| Serpentine Receptor, class H family member (srh-212) [Caenorhabditis elegans] 1.0E-144
blast CAB04743 Hypothetical protein T20B3.5 [Caenorhabditis elegans] ref|NP_507246.1| Serpentine Receptor, class H family member (srh-216) [Caenorhabditis elegans] 4.0E-86
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504010.1 8 20-42,55-77,100-119,140-158,168-190,195-217,248-270,277-299
T22F3.5 8 20-42,55-77,100-119,140-158,168-190,195-217,248-270,277-299
SNAP00000034227 8 20-42,55-77,100-119,140-158,168-190,195-217,248-270,277-299
Sequence
Target Sequence 
>C. ELEGANS:191362_AT
ggttcatcacgcatttcatcgttgcaacagtgttctttttccccgcgtaccaaatgatcc
ccgaccagcaactcgcaaagaactttgtgataagcatagccccatgcattccactttatg
tgaatgctgacctggtttttgtgatgctccttgaaactcgatttttcctccgagcagctg
gtgttctaattttggcggggttcatggagatttggatatttgcatatgttaccgatagat
tgcttgggcaacagataaatcggacaatgtcgaagaggacagtggagttgcacaagaaat
ttcaaaaagcatttctgacccagctaacaatcccaattcttattctcatcctgcccatca
tctatatcggtgtttcgtgcctagtcttctaccacaatcaggcaatcaataatattactg
tgataattgtgtcatcccatggtttcttctcgacaatcgtcatgatttacatacacgcac
cgtaccgcgagtttacgcttcgatggctcacggttttgacgcatgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTTCATCACGCATTTCATCGTTGC 362 507 431 Antisense
ATAAGCATAGCCCCATGCATTCCAC 601 25 520 Antisense
GCTGACCTGGTTTTTGTGATGCTCC 114 297 556 Antisense
GTGATGCTCCTTGAAACTCGATTTT 574 481 571 Antisense
TTCCTCCGAGCAGCTGGTGTTCTAA 57 701 595 Antisense
ACAATCCCAATTCTTATTCTCATCC 198 115 757 Antisense
TATATCGGTGTTTCGTGCCTAGTCT 476 661 793 Antisense
TGCCTAGTCTTCTACCACAATCAGG 34 587 808 Antisense
CATGGTTTCTTCTCGACAATCGTCA 47 209 868 Antisense
TACCGCGAGTTTACGCTTCGATGGC 557 649 913 Antisense
ATGGCTCACGGTTTTGACGCATGTT 534 53 933 Antisense
Sequence Source Affymetrix Proprietary Database