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[191357_s_at]

Description C34F11.9A /REP_DB=WormBase Gene ID /WP=CE27745 /TR=Q18468 /GB=AAA85766.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=dishevelled-like protein
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 03      4grp_high_8_8 > 33      6grp_highest_nonorm > 60      6grp_total > 25      4grp_total > 47      6grp_highest_sub > De      6grp_cgc > 33      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 397
1 2 3 4 range
A 619.3
(3)
P 		416
(-0.5)
P 		271.7
(-3)
P 		390.1
(-1)
P 		348
B 612.2
(3)
P 		271.2
(-2.6)
M 		249.5
(-3)
A 		348.5
(-1.4)
P 		363
C 646
(3)
P 		306.3
(-2.6)
M 		362.2
(-1.7)
P 		284.2
(-3)
P 		362
avr 625.8
(3) 		331.2
(-2.3) 		294.5
(-3) 		340.9
(-2.2) 		331

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 262
1 2 3 4 5 6 range
A 513.5
(3)
P 		468
(1.9)
P 		311.2
(-2)
P 		340.2
(-1.3)
P 		269.8
(-3)
P 		366.6
(-0.6)
P 		244
B 500
(3)
P 		490.3
(2.8)
P 		375.3
(-0.1)
P 		309.7
(-1.7)
P 		306.2
(-1.8)
P 		255.5
(-3)
P 		245
C 517
(3)
P 		512.7
(2.9)
P 		496.2
(2.5)
P 		357.3
(-1.1)
P 		290
(-2.8)
P 		283.6
(-3)
P 		233
avr 510.2
(3) 		490.3
(2.5) 		394.2
(-0.1) 		335.7
(-1.7) 		288.7
(-3) 		301.9
(-2.6) 		222
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 178
1 2 3 4 range
A 225.5
(3)
P 		90.3
(-1.8)
P 		71
(-2.5)
P 		57.1
(-3)
P 		168
B 195.2
(3)
P 		67
(-2.6)
M 		57.8
(-3)
A 		74.3
(-2.3)
P 		137
C 213.9
(3)
P 		88.1
(-1.5)
M 		126.2
(-0.2)
P 		47.9
(-3)
P 		166
avr 211.5
(3) 		81.8
(-2.1) 		85
(-2) 		59.8
(-3) 		152

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 124
1 2 3 4 5 6 range
A 94.7
(-1.4)
P 		166.4
(3)
P 		67.8
(-3)
P 		86.3
(-1.9)
P 		81.4
(-2.2)
P 		89.3
(-1.7)
P 		99
B 174
(3)
P 		163.2
(2.3)
P 		91.1
(-2.4)
P 		82
(-3)
P 		124.4
(-0.2)
P 		95.3
(-2.1)
P 		92
C 191.6
(3)
P 		156.4
(1.2)
P 		126.3
(-0.3)
P 		71.9
(-3)
P 		93.8
(-1.9)
P 		94.9
(-1.8)
P 		120
avr 153.4
(2.4) 		162
(3) 		95.1
(-1.9) 		80.1
(-3) 		99.9
(-1.5) 		93.2
(-2) 		82
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191357_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5559
Sequence Type Exemplar sequence
Representative Public ID C34F11.9A NCBI
Target Description C34F11.9A /REP_DB=WormBase Gene ID /WP=CE27745 /TR=Q18468 /GB=AAA85766.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=dishevelled-like protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062577(10), NM_062578(10), NM_062579(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062577 NCBI Caenorhabditis elegans DiSHevelled related family member (dsh-1) (dsh-1) mRNA, complete cds. 10/11 A 
NM_062578 NCBI Caenorhabditis elegans DiSHevelled related family member (dsh-1) (dsh-1) mRNA, complete cds. 10/11 None
NM_062579 NCBI Caenorhabditis elegans DiSHevelled related family member (dsh-1) (dsh-1) mRNA, complete cds. 10/11 None
SNAP00000021336 ENSEMBL cdna:SNAP chromosome:CEL140:II:5215602:5217455:-1 9/11 None
GENEFINDER00000021353 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5215602:5219419:-1 9/11 None
C34F11.9c ENSEMBL cdna:known chromosome:CEL140:II:5214420:5225345:-1 gene:C34F11.9 10/11 A 
C34F11.9a ENSEMBL cdna:known chromosome:CEL140:II:5214777:5225350:-1 gene:C34F11.9 10/11 None
C34F11.9b ENSEMBL cdna:known chromosome:CEL140:II:5215602:5219880:-1 gene:C34F11.9 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5215599-5225343(-) 100.0 100.0
Public Domain and Genome References
Ensembl C34F11.9
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM98049 Dishevelled related protein 1, isoform c [Caenorhabditis elegans] ref|NP_494978.2| DiSHevelled related family member (dsh-1) [Caenorhabditis elegans] 0.0
blast AAM98047 Dishevelled related protein 1, isoform a [Caenorhabditis elegans] ref|NP_494979.3| DiSHevelled related family member (dsh-1) [Caenorhabditis elegans] 0.0
blast AAM98048 Dishevelled related protein 1, isoform b [Caenorhabditis elegans] ref|NP_494980.2| DiSHevelled related family member (dsh-1) [Caenorhabditis elegans] 0.0
blast T15775 hypothetical protein C34F11.9b - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.6E-25
Pfam IPR000591 EMBL-EBI Pleckstrin putative G-protein interacting domain 2.4E-21
Pfam IPR001158 EMBL-EBI DIX domain 3.9E-41
Pfam IPR003351 EMBL-EBI Dishevelled protein 1.6E-21
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.6E-25
Pfam IPR000591 EMBL-EBI Pleckstrin putative G-protein interacting domain 2.4E-21
Pfam IPR003351 EMBL-EBI Dishevelled protein 4.5E-39
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.6E-25
Pfam IPR000591 EMBL-EBI Pleckstrin putative G-protein interacting domain 1.5E-18
Pfam IPR001478 EMBL-EBI PDZ/DHR/GLGF domain 1.6E-25
Pfam IPR003351 EMBL-EBI Dishevelled protein 7.4E-27
Sequence
Target Sequence 
>C. ELEGANS:191357_S_AT
acgagaagctgttagccgtcgtggccccatcaaattaactgtggcgaaaagttttgaaaa
tgggcaaagttgtttcacaataccaaggaatagcagagaagagcctgtgagaccaatcga
tactcaagcatggatccagcatacgaatgcaatgagaggaatgccaagtatagttgaagg
atctgatctggtcgaatggcttttggatcatgttgaagggcttcgagaacggaagacagc
acgaaactttgcggcggatttactgaaattaaagtatattgcacatgtggtgaacaaggt
cacattcactgagcagtgctattatgtccttggagatgagtgttcaggtaggaaagtata
catttattcggaaaatgtcaaagtttttgtatatgaagatggaaactatgcccgattccg
aaatgaagatggtggacccaaataccaatggaccatcggaatgaacggaatgtctgctgg
caacggaagctctgtgatgctcccacccccacatttacctggaggtatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGAGAAGCTGTTAGCCGTCGTGGC 479 93 1686 Antisense
TCGTGGCCCCATCAAATTAACTGTG 227 287 1704 Antisense
AGAGCCTGTGAGACCAATCGATACT 586 69 1785 Antisense
GGATCTGATCTGGTCGAATGGCTTT 288 511 1864 Antisense
GACAGCACGAAACTTTGCGGCGGAT 242 367 1920 Antisense
GAACAAGGTCACATTCACTGAGCAG 325 353 1977 Antisense
GAGCAGTGCTATTATGTCCTTGGAG 143 389 1996 Antisense
GAAACTATGCCCGATTCCGAAATGA 657 355 2087 Antisense
TGAACGGAATGTCTGCTGGCAACGG 573 573 2147 Antisense
GCAACGGAAGCTCTGTGATGCTCCC 363 319 2165 Antisense
ACCCCCACATTTACCTGGAGGTATG 134 89 2190 Antisense
Sequence Source Affymetrix Proprietary Database