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[191338_at] 7TM chemoreceptor, sri family

Description F33H12.4 /REP_DB=WormBase Gene ID /WP=CE25867 /GEN=sri-74 /TR=O44840 /GB=AAB95004.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster 6grp_total > 34      4grp_total > 06      6grp_cgc > 34      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 39.6
(0.2)
A 		1.7
(-3)
A 		8.8
(-2.4)
A 		73.6
(3)
P 		72
B 50.5
(2.7)
P 		26.5
(-1.6)
A 		18.5
(-3)
A 		52.2
(3)
P 		34
C 30.9
(-0.2)
M 		3.7
(-3)
A 		22
(-1.1)
A 		62.8
(3)
P 		59
avr 40.3
(0.4) 		10.6
(-3) 		16.4
(-2.3) 		62.9
(3) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 81
1 2 3 4 5 6 range
A 29.2
(-0.5)
A 		53.3
(2.8)
P 		54.7
(3)
A 		10.4
(-3)
A 		25.6
(-0.9)
A 		17
(-2.1)
A 		44
B 87.1
(3)
P 		27.1
(-1.4)
P 		39.2
(-0.6)
P 		21.3
(-1.9)
A 		6.2
(-3)
A 		32.7
(-1)
A 		81
C 34.5
(1.7)
A 		41.7
(3)
P 		27.4
(0.4)
A 		8.4
(-3)
A 		21
(-0.7)
A 		28.4
(0.6)
A 		33
avr 50.3
(3) 		40.7
(1.4) 		40.4
(1.4) 		13.4
(-3) 		17.6
(-2.3) 		26
(-1) 		37
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 14.4
(3)
A 		0.4
(-3)
A 		2.3
(-2.2)
A 		10.8
(1.5)
P 		14
B 16.1
(3)
P 		6.6
(-1.8)
A 		4.3
(-3)
A 		11.1
(0.5)
P 		12
C 10.2
(2.7)
M 		1.1
(-3)
A 		7.7
(1.2)
A 		10.6
(3)
P 		10
avr 13.6
(3) 		2.7
(-3) 		4.8
(-1.8) 		10.8
(1.5) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 5.4
(-2)
A 		19
(3)
P 		11.9
(0.4)
A 		2.6
(-3)
A 		7.7
(-1.1)
A 		4.1
(-2.5)
A 		16
B 30.3
(3)
P 		9
(-1.6)
P 		9.5
(-1.5)
P 		5.6
(-2.3)
A 		2.5
(-3)
A 		12.2
(-0.9)
A 		28
C 12.8
(3)
A 		12.7
(2.9)
P 		7
(-0.1)
A 		1.7
(-3)
A 		6.8
(-0.2)
A 		9.5
(1.2)
A 		11
avr 16.2
(3) 		13.6
(1.8) 		9.5
(-0.1) 		3.3
(-3) 		5.7
(-1.9) 		8.6
(-0.5) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191338_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4447
Sequence Type Exemplar sequence
Representative Public ID F33H12.4 NCBI
Target Description F33H12.4 /REP_DB=WormBase Gene ID /WP=CE25867 /GEN=sri-74 /TR=O44840 /GB=AAB95004.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061898(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061898 NCBI Caenorhabditis elegans Serpentine Receptor, class I family member (sri-74) (sri-74) mRNA, complete cds. 11/11 None
GENEFINDER00000001669 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2580902:2586823:-1 11/11 None
F33H12.4 ENSEMBL cdna:known chromosome:CEL140:II:2580902:2583007:-1 gene:F33H12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2580900-2583006(-) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, sri family
Gene Symbol sri-74
Ensembl F33H12.4
Entrez Gene 191930 Entrez gene
SwissProt O44840 EMBL-EBI
WormBase CE25867 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast T32831 hypothetical protein F33H12.5 - Caenorhabditis elegans 0.0
blast AAB95004 Serpentine receptor, class i protein 74 [Caenorhabditis elegans] ref|NP_494299.1| Serpentine Receptor, class I family member (sri-74) [Caenorhabditis elegans] 1.0E-174
blast AAB95004 Serpentine receptor, class i protein 74 [Caenorhabditis elegans] ref|NP_494299.1| Serpentine Receptor, class I family member (sri-74) [Caenorhabditis elegans] 1.0E-142
blast NP_494304 Serpentine Receptor, class I family member (sri-77) [Caenorhabditis elegans] gb|AAB66070.2| Serpentine receptor, class i protein 77 [Caenorhabditis elegans] 1.0E-134
blast AAK72087 Serpentine receptor, class i protein 36 [Caenorhabditis elegans] ref|NP_494300.1| Serpentine Receptor, class I family member (sri-36) [Caenorhabditis elegans] 1.0E-114
blast AAB94259 Serpentine receptor, class i protein 73 [Caenorhabditis elegans] ref|NP_503247.2| Serpentine Receptor, class I family member (sri-73) [Caenorhabditis elegans] 2.0E-88
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-23
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 5.8E-70
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 2.2E-91
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_494299.1 7 15-37,49-71,91-113,133-155,193-215,243-265,278-300
F33H12.4 7 15-37,49-71,91-113,133-155,193-215,243-265,278-300
GENEFINDER00000001669 12 201-223,238-260,281-300,338-360,390-412,427-444,465-487,497-516,536-558,596-618,646-668,681-703
Sequence
Target Sequence 
>C. ELEGANS:191338_AT
ttctatgtgctttattctcccaattgttatgtgtgtatgggtaaattcgctcagtctcag
tcgagaagaggcgctggtctatattaaagaggtatatccaaaagggtatttgcaattttc
gaagctaccaaattttgtggtctacgtgaaatctccgtatacaacactacttatctcagc
gctttttatagccggtagtttaggatttgcgtgtctatctctattcatttatgatgttgt
acgtatgatggctcacttgaagcttcgaatctcaaaactaacatatgaaatgcacaatga
agcgcttcgaagtgtgatcatacagttcataaccgcagtgttgtgtctgcttggtccaag
gttactgcttctagtcttagtttttgaaattccatacatgaatttaatatctgaactatt
gtttgcctggtttgcaagtcattcgtcagttaacatgatttctttattcatattctttcc
gccatatcccaagattatcgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCTATGTGCTTTATTCTCCCAATT 110 691 426 Antisense
ATGGGTAAATTCGCTCAGTCTCAGT 147 51 462 Antisense
ACAACACTACTTATCTCAGCGCTTT 51 113 586 Antisense
ATCTCAGCGCTTTTTATAGCCGGTA 634 31 598 Antisense
ATTTGCGTGTCTATCTCTATTCATT 577 17 630 Antisense
GATGTTGTACGTATGATGGCTCACT 473 409 658 Antisense
GGCTCACTTGAAGCTTCGAATCTCA 575 537 675 Antisense
ACCGCAGTGTTGTGTCTGCTTGGTC 400 87 757 Antisense
GTCCAAGGTTACTGCTTCTAGTCTT 524 449 779 Antisense
GGTTTGCAAGTCATTCGTCAGTTAA 300 503 854 Antisense
TTCCGCCATATCCCAAGATTATCGC 194 699 902 Antisense
Sequence Source Affymetrix Proprietary Database