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[191331_at] glutathione S-transferase

Description Y53F4B.31 /REP_DB=WormBase Gene ID /WP=CE22418 /TR=Q9NAB2 /GB=CAB61105.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=glutathione-S-transferase
Cluster 6grp_highest_10_10 > 34      6grp_high1_10_10 > 31      4grp_highest_8_8 > 08      4grp_high_8_8 > 53      6grp_highest_nonorm > 22      6grp_total > 21      4grp_total > 51      6grp_highest_sub > 02_03      6grp_cgc > 44      6grp_high1_sub > 03      6grp_cgc_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1627
1 2 3 4 range
A 1013.5
(-0.1)
P 		1802.3
(3)
P 		1473.3
(1.7)
P 		254.9
(-3)
P 		1547
B 902.1
(-0.5)
P 		1857.4
(3)
P 		1483.2
(1.6)
P 		230.9
(-3)
P 		1627
C 1144.2
(0.8)
P 		1649.5
(3)
P 		1429.9
(2.1)
P 		248.7
(-3)
P 		1401
avr 1019.9
(0) 		1769.7
(3) 		1462.1
(1.8) 		244.8
(-3) 		1525

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1410
1 2 3 4 5 6 range
A 1396
(0.2)
P 		1915.7
(3)
P 		1899
(2.9)
P 		1110.2
(-1.3)
P 		939
(-2.2)
P 		781.9
(-3)
P 		1134
B 1177.9
(-1.7)
P 		1790.2
(1.1)
P 		2192.1
(3)
P 		1036.4
(-2.3)
P 		1105.6
(-2)
P 		895.4
(-3)
P 		1297
C 1268.3
(-0.8)
P 		1765.2
(3)
P 		1683.7
(2.4)
P 		985.1
(-3)
P 		1024.6
(-2.7)
P 		1140
(-1.8)
P 		780
avr 1280.7
(-0.9) 		1823.7
(2.4) 		1924.9
(3) 		1043.9
(-2.4) 		1023.1
(-2.5) 		939.1
(-3) 		986
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 461
1 2 3 4 range
A 369
(2.6)
P 		391.2
(3)
P 		385.1
(2.9)
P 		37.3
(-3)
P 		354
B 287.7
(0.5)
P 		458.7
(3)
P 		343.7
(1.3)
P 		49.2
(-3)
P 		410
C 378.8
(1.4)
P 		474.2
(2.7)
P 		498.4
(3)
P 		41.9
(-3)
P 		457
avr 345.2
(1.6) 		441.4
(3) 		409.1
(2.5) 		42.8
(-3) 		399

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 491
1 2 3 4 5 6 range
A 257.3
(-2.2)
P 		681.3
(3)
P 		413.6
(-0.3)
P 		281.6
(-1.9)
P 		283.4
(-1.9)
P 		190.4
(-3)
P 		491
B 409.8
(-0.5)
P 		595.9
(3)
P 		532.3
(1.8)
P 		274.5
(-3)
P 		449.2
(0.3)
P 		333.9
(-1.9)
P 		321
C 470.1
(1.8)
P 		538.6
(3)
P 		428.5
(1.1)
P 		198.1
(-3)
P 		331.5
(-0.6)
P 		381.6
(0.2)
P 		341
avr 379.1
(-0.8) 		605.3
(3) 		458.1
(0.5) 		251.4
(-3) 		354.7
(-1.2) 		302
(-2.1) 		354
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191331_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2827
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.31 NCBI
Target Description Y53F4B.31 /REP_DB=WormBase Gene ID /WP=CE22418 /TR=Q9NAB2 /GB=CAB61105.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=glutathione-S-transferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064716(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064716 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-28) (gst-28) mRNA, complete cds. 11/11 None
SNAP00000043069 ENSEMBL cdna:SNAP chromosome:CEL140:II:15166161:15167282:-1 11/11 None
GENEFINDER00000043132 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:15166161:15167282:-1 11/11 None
Y53F4B.31.2 ENSEMBL cdna:known chromosome:CEL140:II:15166101:15167298:-1 gene:Y53F4B.31 11/11 None
Y53F4B.31.1 ENSEMBL cdna:known chromosome:CEL140:II:15166102:15167296:-1 gene:Y53F4B.31 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:15166157-15167279(-) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-28
Ensembl Y53F4B.31
Entrez Gene 190224 Entrez gene
SwissProt Q9NAB2 EMBL-EBI
WormBase CE22418 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1605410_AT similar to Glutathione-requiring prostaglandin D synthase (Glutathione-dependent PGD synthetase) (Prostaglandin-H2 D-isomerase) (Hematopoietic prostaglandin D synthase) (H-PGDS) cfa
CANINE_2:CFA.1584.1.S1_AT similar to Glutathione-requiring prostaglandin D synthase (Glutathione-dependent PGD synthetase) (Prostaglandin-H2 D-isomerase) (Hematopoietic prostaglandin D synthase) (H-PGDS) cfa
CANINE_2:CFA.1584.2.S1_S_AT similar to Glutathione-requiring prostaglandin D synthase (Glutathione-dependent PGD synthetase) (Prostaglandin-H2 D-isomerase) (Hematopoietic prostaglandin D synthase) (H-PGDS) cfa
DROSOPHILA_2:1623957_S_AT Glutathione S transferase S1 dm
DROSGENOME1:153990_AT Glutathione S transferase S1 dm
CHICKEN:GGA.15142.1.S1_S_AT prostaglandin-D synthase gga
CHICKEN:GGA.3137.1.S1_AT prostaglandin-D synthase gga
HG-U133_PLUS_2:206726_AT prostaglandin D2 synthase, hematopoietic hs
HG-U133A_2:206726_AT prostaglandin D2 synthase, hematopoietic hs
HG-U133A:206726_AT prostaglandin D2 synthase, hematopoietic hs
HG-FOCUS:206726_AT prostaglandin D2 synthase, hematopoietic hs
U133_X3P:G7657456_3P_AT prostaglandin D2 synthase, hematopoietic hs
HG-U95AV2:35523_AT prostaglandin D2 synthase, hematopoietic hs
MOE430A:1421492_AT prostaglandin D2 synthase 2, hematopoietic mm
MOUSE430A_2:1421492_AT prostaglandin D2 synthase 2, hematopoietic mm
MOUSE430_2:1421492_AT prostaglandin D2 synthase 2, hematopoietic mm
RG-U34A:AF021882_S_AT prostaglandin D2 synthase 2 rn
RG-U34A:D82071_AT prostaglandin D2 synthase 2 rn
RAE230A:1368492_AT prostaglandin D2 synthase 2 rn
RAT230_2:1368492_AT prostaglandin D2 synthase 2 rn
Protein Similarities
Method ID Description E-Value
blast CAB61105 Hypothetical protein Y53F4B.31 [Caenorhabditis elegans] ref|NP_497117.1| Glutathione S-Transferase family member (gst-28) [Caenorhabditis elegans] 1.0E-117
blast CAB61107 Hypothetical protein Y53F4B.33 [Caenorhabditis elegans] ref|NP_497119.1| Glutathione S-Transferase family member (gst-39) [Caenorhabditis elegans] 4.0E-95
blast CAE73239 Hypothetical protein CBG20648 [Caenorhabditis briggsae] 4.0E-95
Protein Domains
Database ID Description E-Value
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 3.4E-12
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 2.4E-15
Sequence
Target Sequence 
>C. ELEGANS:191331_AT
ctacctacaagctcacctatttcaatgctcgtggatacgctgaggccgcacgtatcctct
tccacttggcagatgttccattcgaggacttccgtatgacacacggagatggaacatggg
agaagcttaaagacggcgccccatttggtcaagtaccttacctgaccgttgatggatttg
atatcccacaatctgctgcaataatccgctaccttgccaacaaattcggatatgctggaa
aaaccccagaagagcaggtttgggcagatgccatcgttgatcagttcaaggatttcatgg
gatcattccgcgaacgtatcatggcacatttcgctggaaaatctcaggaagaaattgcaa
agatcacttctgaagtagtgattccagctagagattcgtactttaagattctcaatgggc
ttcttgaaaagagcaagtcaggattcctagtcggcgacagtatcaccttcgcggacattg
tcatctctgagaatattactactttggagaaaaatcaattgtttgttgcttcggagcaac
caaacttggctgctcttcgagaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTACCTACAAGCTCACCTATTTCAA 241 213 17 Antisense
CTATTTCAATGCTCGTGGATACGCT 177 221 33 Antisense
GATACGCTGAGGCCGCACGTATCCT 464 423 50 Antisense
TATCCTCTTCCACTTGGCAGATGTT 201 659 69 Antisense
GATGTTCCATTCGAGGACTTCCGTA 506 409 88 Antisense
AGTACCTTACCTGACCGTTGATGGA 520 1 168 Antisense
GGATTTCATGGGATCATTCCGCGAA 153 509 306 Antisense
ATTCCGCGAACGTATCATGGCACAT 268 9 321 Antisense
TAGTCGGCGACAGTATCACCTTCGC 289 653 464 Antisense
CCTTCGCGGACATTGTCATCTCTGA 637 269 482 Antisense
CCAAACTTGGCTGCTCTTCGAGAGA 642 275 556 Antisense
Sequence Source Affymetrix Proprietary Database