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[191307_at] glutathione S-transferase

Description Y53F4B.35 /REP_DB=WormBase Gene ID /WP=CE22421 /TR=Q9NAA9 /GB=CAB61109.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=glutathione-S-transferase
Cluster 6grp_total > 17      4grp_total > 39      6grp_cgc > 11      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 293
1 2 3 4 range
A 334.4
(3)
P 		52.6
(-3)
A 		56
(-2.9)
A 		144
(-1.1)
P 		282
B 301.7
(3)
P 		68
(-2.4)
A 		41.2
(-3)
A 		115.9
(-1.3)
M 		261
C 285.9
(3)
P 		93.1
(-2.3)
A 		67.1
(-3)
A 		107.4
(-1.9)
P 		219
avr 307.3
(3) 		71.2
(-2.6) 		54.8
(-3) 		122.4
(-1.4) 		253

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1204
1 2 3 4 5 6 range
A 1058.9
(3)
P 		327.8
(-1.4)
P 		314
(-1.4)
P 		150.3
(-2.4)
P 		115.7
(-2.6)
P 		52.4
(-3)
A 		1007
B 1256.4
(3)
P 		425.3
(-1.2)
P 		290.3
(-1.9)
P 		141.4
(-2.6)
P 		104.4
(-2.8)
P 		72.3
(-3)
A 		1184
C 1147.5
(3)
P 		354.5
(-1.5)
P 		249.1
(-2)
P 		137
(-2.7)
P 		78.5
(-3)
P 		132.1
(-2.7)
A 		1069
avr 1154.3
(3) 		369.2
(-1.4) 		284.5
(-1.9) 		142.9
(-2.7) 		99.5
(-2.9) 		85.6
(-3) 		1069
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 112
1 2 3 4 range
A 121.7
(3)
P 		11.4
(-3)
A 		14.6
(-2.8)
A 		21.1
(-2.5)
P 		110
B 96.2
(3)
P 		16.8
(-2.5)
A 		9.5
(-3)
A 		24.7
(-1.9)
M 		87
C 94.7
(3)
P 		26.8
(-2.3)
A 		23.4
(-2.6)
A 		18.1
(-3)
P 		77
avr 104.2
(3) 		18.3
(-2.8) 		15.8
(-3) 		21.3
(-2.6) 		88

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 424
1 2 3 4 5 6 range
A 195.2
(3)
P 		116.6
(0.4)
P 		68.4
(-1.2)
P 		38.1
(-2.2)
P 		34.9
(-2.3)
P 		12.8
(-3)
A 		182
B 437.1
(3)
P 		141.6
(-1.3)
P 		70.5
(-2.4)
P 		37.5
(-2.8)
P 		42.4
(-2.8)
P 		27
(-3)
A 		410
C 425.3
(3)
P 		108.2
(-1.8)
P 		63.4
(-2.4)
P 		27.6
(-3)
P 		25.4
(-3)
P 		44.2
(-2.7)
A 		400
avr 352.5
(3) 		122.1
(-1.3) 		67.4
(-2.3) 		34.4
(-2.9) 		34.2
(-2.9) 		28
(-3) 		325
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191307_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3380
Sequence Type Exemplar sequence
Representative Public ID Y53F4B.35 NCBI
Target Description Y53F4B.35 /REP_DB=WormBase Gene ID /WP=CE22421 /TR=Q9NAA9 /GB=CAB61109.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=glutathione-S-transferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064720(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064720 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-31) (gst-31) mRNA, complete cds. 11/11 None
SNAP00000043074 ENSEMBL cdna:SNAP chromosome:CEL140:II:15174161:15174837:-1 11/11 None
GENEFINDER00000043130 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:15174161:15174837:-1 11/11 None
Y53F4B.35 ENSEMBL cdna:known chromosome:CEL140:II:15174161:15174837:-1 gene:Y53F4B.35 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:15174157-15174834(-) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-31
Ensembl Y53F4B.35
Entrez Gene 190228 Entrez gene
SwissProt Q9NAA9 EMBL-EBI
WormBase CE22421 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB61109 Hypothetical protein Y53F4B.35 [Caenorhabditis elegans] ref|NP_497121.1| Glutathione S-Transferase family member (gst-31) [Caenorhabditis elegans] 1.0E-118
blast NP_503532 Glutathione S-Transferase family member (gst-37) [Caenorhabditis elegans] gb|AAG53708.1| Glutathione s-transferase protein 37 [Caenorhabditis elegans] 3.0E-86
blast CAB61107 Hypothetical protein Y53F4B.33 [Caenorhabditis elegans] ref|NP_497119.1| Glutathione S-Transferase family member (gst-39) [Caenorhabditis elegans] 5.0E-86
Protein Domains
Database ID Description E-Value
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 4.7E-10
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 7.6E-18
Sequence
Target Sequence 
>C. ELEGANS:191307_AT
ctacaagctgacctacttcaactctcgattctacgccgaaccggctcgcattctcttcca
tcttgcgggggtcccatttgaagacatccgaatcatccatggagatggtacttgggagca
gattaaggataaaactccatttggtcaagtacctgttctgaacatcaatggatttgaaat
tcctcaatctactgcaatcatccgctaccttgcaaccaaattcggatacgctgggaaaac
cccggaagagcaggcttgggctgatgcaatttgtgatcaagtcagagactttatggcagt
tccatttaaagatattgtagtggcaaaacgtgttggaaagacagaagaggaagtgtcaca
gcttataaatgaaggattcgtccctggcagagatgcttacttcaaaatcatcacaaggat
ccttgagaagaacaagtcaggtttccttgttggtgatggaatcacattcgccgatattgt
tgtcgtggagaatttgaccacgttgcagaagaatcagctctttgaagcttcgaaatatcc
agaactggcagctctcagag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTACAAGCTGACCTACTTCAACTCT 317 227 21 Antisense
CTACTTCAACTCTCGATTCTACGCC 564 225 33 Antisense
GCGGGGGTCCCATTTGAAGACATCC 647 291 85 Antisense
GAAGACATCCGAATCATCCATGGAG 679 339 100 Antisense
TGGTCAAGTACCTGTTCTGAACATC 584 549 162 Antisense
TCCTCAATCTACTGCAATCATCCGC 121 593 201 Antisense
CGCTACCTTGCAACCAAATTCGGAT 291 255 223 Antisense
GCAGGCTTGGGCTGATGCAATTTGT 222 311 270 Antisense
AGGATTCGTCCCTGGCAGAGATGCT 407 57 393 Antisense
GGAGAATTTGACCACGTTGCAGAAG 261 519 507 Antisense
TATCCAGAACTGGCAGCTCTCAGAG 147 659 556 Antisense
Sequence Source Affymetrix Proprietary Database