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[191306_s_at] choline O-acetyltransferase

Description B0395.3 /REP_DB=WormBase Gene ID /WP=CE02944 /TR=Q17499 /GB=CAA92218.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=choline O-acetyltransferase
Cluster 6grp_highest_10_10 > 74      6grp_high1_10_10 > 47      4grp_highest_8_8 > 12      4grp_high_8_8 > 39      6grp_highest_nonorm > 77      6grp_total > 47      4grp_total > 40      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 651
1 2 3 4 range
A 1244.2
(-3)
P 		1281.9
(-2.5)
P 		1664.7
(3)
P 		1534.7
(1.1)
P 		421
B 1122.4
(-3)
P 		1484.5
(0.5)
P 		1736
(3)
P 		1629.9
(2)
P 		614
C 1085.4
(-3)
P 		1449.7
(1)
P 		1626.2
(3)
P 		1358.9
(0)
P 		541
avr 1150.7
(-3) 		1405.4
(-0.1) 		1675.6
(3) 		1507.8
(1.1) 		525

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 896
1 2 3 4 5 6 range
A 1559.3
(-1.1)
P 		1596
(-0.8)
P 		1282.7
(-3)
P 		1471.8
(-1.7)
P 		1381.2
(-2.3)
P 		2134.2
(3)
P 		852
B 1820.2
(3)
P 		1475.8
(-0.6)
P 		1512.2
(-0.2)
P 		1499.8
(-0.3)
P 		1238.3
(-3)
P 		1483.3
(-0.5)
P 		582
C 1865.7
(2.6)
P 		1300.3
(-2.8)
P 		1905.7
(3)
P 		1276.4
(-3)
P 		1447
(-1.4)
P 		1650.3
(0.6)
P 		629
avr 1748.4
(2.9) 		1457.4
(-1.5) 		1566.9
(0.2) 		1416
(-2.1) 		1355.5
(-3) 		1755.9
(3) 		400
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 342
1 2 3 4 range
A 453
(3)
P 		278.3
(-1.6)
P 		435.2
(2.5)
P 		224.6
(-3)
P 		228
B 357.9
(-1.8)
P 		366.6
(-0.9)
P 		402.3
(3)
P 		347.3
(-3)
P 		55
C 359.3
(-0.7)
P 		416.8
(0.3)
P 		566.8
(3)
P 		229.1
(-3)
P 		338
avr 390.1
(0.7) 		353.9
(-0.4) 		468.1
(3) 		267
(-3) 		201

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 435
1 2 3 4 5 6 range
A 287.4
(-2.8)
P 		567.6
(3)
P 		279.3
(-3)
P 		373.3
(-1)
P 		416.8
(-0.1)
P 		519.6
(2)
P 		288
B 633.3
(3)
P 		491.2
(-0.2)
P 		367.2
(-3)
P 		397.2
(-2.3)
P 		503.1
(0.1)
P 		553.1
(1.2)
P 		266
C 691.5
(3)
P 		396.8
(-1.1)
P 		485
(0.2)
P 		256.7
(-3)
P 		468.2
(-0.1)
P 		552.5
(1.1)
P 		435
avr 537.4
(2.9) 		485.2
(1.3) 		377.2
(-2) 		342.4
(-3) 		462.7
(0.6) 		541.7
(3) 		199
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191306_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18025
Sequence Type Exemplar sequence
Representative Public ID B0395.3 NCBI
Target Description B0395.3 /REP_DB=WormBase Gene ID /WP=CE02944 /TR=Q17499 /GB=CAA92218.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=choline O-acetyltransferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_078223(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_078223 NCBI Caenorhabditis elegans B0395.3 (B0395.3) mRNA, complete cds. 11/11 None
SNAP00000033251 ENSEMBL cdna:SNAP chromosome:CEL140:X:16035138:16039402:-1 11/11 None
GENEFINDER00000033254 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:16035138:16039145:-1 11/11 None
B0395.3.1 ENSEMBL cdna:known chromosome:CEL140:X:16034977:16039152:-1 gene:B0395.3 11/11 None
B0395.3.3 ENSEMBL cdna:known chromosome:CEL140:X:16034982:16039428:-1 gene:B0395.3 11/11 None
B0395.3.2 ENSEMBL cdna:known chromosome:CEL140:X:16034982:16039154:-1 gene:B0395.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:16035137-16039139(-) 100.0 100.0
Public Domain and Genome References
Gene Title choline O-acetyltransferase
Ensembl B0395.3
Entrez Gene 181682 Entrez gene
SwissProt Q17499 EMBL-EBI
WormBase CE02944 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.21223.1.S1_S_AT similar to carnitine acetyltransferase isoform 1 precursor cfa
DROSOPHILA_2:1624185_AT dm
DROSGENOME1:149399_AT dm
CHICKEN:GGAAFFX.2762.2.S1_AT similar to carnitine acetyltransferase gga
CHICKEN:GGAAFFX.2762.2.S1_S_AT similar to carnitine acetyltransferase gga
HG-U133_PLUS_2:209522_S_AT carnitine acetyltransferase hs
HG-U133A:209522_S_AT carnitine acetyltransferase hs
HG-FOCUS:209522_S_AT carnitine acetyltransferase hs
HG-U133A_2:209522_S_AT carnitine acetyltransferase hs
HU35KSUBC:RC_T12603_AT carnitine acetyltransferase hs
HU35KSUBD:RC_AA621218_S_AT carnitine acetyltransferase hs
HUGENEFL:X78706_AT carnitine acetyltransferase hs
HG-U133A:205843_X_AT carnitine acetyltransferase hs
HG-U133_PLUS_2:205843_X_AT carnitine acetyltransferase hs
HG-U133A_2:205843_X_AT carnitine acetyltransferase hs
U133_X3P:G4557486_3P_A_AT carnitine acetyltransferase hs
HG-U95AV2:41648_AT carnitine acetyltransferase hs
HG-U95C:63719_F_AT carnitine acetyltransferase hs
HG-U95D:83625_AT carnitine acetyltransferase hs
MU11KSUBA:AA268904_F_AT carnitine acetyltransferase mm
MU11KSUBB:MSA.2644.0_S_AT carnitine acetyltransferase mm
MG-U74AV2:103646_AT carnitine acetyltransferase mm
MG-U74BV2:116424_AT carnitine acetyltransferase mm
MG-U74AV2:161989_F_AT carnitine acetyltransferase mm
MOE430A:1448544_AT carnitine acetyltransferase mm
MOUSE430A_2:1448544_AT carnitine acetyltransferase mm
MOUSE430_2:1448544_AT carnitine acetyltransferase mm
MOE430A:1417008_AT carnitine acetyltransferase mm
MOUSE430A_2:1417008_AT carnitine acetyltransferase mm
MOUSE430_2:1417008_AT carnitine acetyltransferase mm
MOE430B:1441919_X_AT carnitine acetyltransferase mm
MOUSE430_2:1441919_X_AT carnitine acetyltransferase mm
MU19KSUBB:TC30021_AT Carnitine acetyltransferase, mRNA (cDNA clone MGC:6046 IMAGE:3499169) mm
MU19KSUBB:TC32836_AT Carnitine acetyltransferase, mRNA (cDNA clone MGC:6046 IMAGE:3499169) mm
MU19KSUBB:TC32836_G_AT Carnitine acetyltransferase, mRNA (cDNA clone MGC:6046 IMAGE:3499169) mm
RG-U34B:RC_AA925306_AT carnitine acetyltransferase rn
RAE230A:1371886_AT carnitine acetyltransferase rn
RAT230_2:1371886_AT carnitine acetyltransferase rn
YEAST_2:1774377_AT Carnitine acetyl-CoA transferase present in both mitochondria and peroxisomes, transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
8415 acyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92218 Hypothetical protein B0395.3 [Caenorhabditis elegans] ref|NP_510624.1| B0395.3 [Caenorhabditis elegans] 0.0
blast CAE70001 Hypothetical protein CBG16408 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000542 EMBL-EBI Acyltransferase ChoActase/COT/CPT 1.0E-126
Pfam IPR000542 EMBL-EBI Acyltransferase ChoActase/COT/CPT 1.0E-126
Pfam IPR000542 EMBL-EBI Acyltransferase ChoActase/COT/CPT 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:191306_S_AT
gactcgttcatccaaatggcttaccaattagctttctaccggatccactcatcgcttcca
ccaacctacgagacggccactttgcgtaaattcaccgagggtcgaaccgagaacatccga
tctccgaatacagctgcagcatcatttgtcaagaagatggtatctaagcctcctacgccg
gttagtgaaatctacgatgcacttgttcacgcaacaaattcgcataaaaagtacaccatg
gattgtatggatgcagctggaatggacagacatttattggcatggaagttgatcgctact
gaaaataatctcccaaccccagaaatcttcggttccaacatctaccaacaactcaaccat
ttccaagtctccacaagtcaagttcccacacggcatttcatccaaatgtgcttcggacca
tctgcccttgattgctatgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTCGTTCATCCAAATGGCTTACC 595 373 1255 Antisense
ACCAATTAGCTTTCTACCGGATCCA 237 75 1277 Antisense
GACGGCCACTTTGCGTAAATTCACC 236 375 1326 Antisense
AACATCCGATCTCCGAATACAGCTG 568 137 1366 Antisense
GAAGATGGTATCTAAGCCTCCTACG 199 339 1407 Antisense
TCTACGATGCACTTGTTCACGCAAC 271 615 1445 Antisense
GAAATCTTCGGTTCCAACATCTACC 138 363 1576 Antisense
TACCAACAACTCAACCATTTCCAAG 132 649 1597 Antisense
CATTTCCAAGTCTCCACAAGTCAAG 507 213 1612 Antisense
TCCAAATGTGCTTCGGACCATCTGC 292 587 1655 Antisense
ACCATCTGCCCTTGATTGCTATGGA 618 85 1671 Antisense
Sequence Source Affymetrix Proprietary Database