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[191305_at] phosphoglycerate kinase

Description T03F1.3 /REP_DB=WormBase Gene ID /WP=CE13100 /TR=SW:P91427 /GB=AAB42230.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=phosphoglycerate kinase
Cluster 6grp_highest_10_10 > 14      6grp_high1_10_10 > 31      4grp_highest_8_8 > 24      4grp_high_8_8 > 51      6grp_highest_nonorm > 26      6grp_total > 42      4grp_total > 60      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5918
1 2 3 4 range
A 6722.4
(3)
P 		5930.8
(2)
P 		6450.9
(2.7)
P 		2001.5
(-3)
P 		4721
B 6531.6
(2.3)
P 		6253.4
(2)
P 		7081.2
(3)
P 		2073.3
(-3)
P 		5008
C 6629.4
(2)
P 		7622.2
(3)
P 		6573.1
(1.9)
P 		1704.6
(-3)
P 		5918
avr 6627.8
(2.9) 		6602.1
(2.9) 		6701.7
(3) 		1926.5
(-3) 		4775

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4087
1 2 3 4 5 6 range
A 6235.5
(0.7)
P 		6297.7
(0.8)
P 		7335.4
(3)
P 		6352.4
(0.9)
P 		4813.3
(-2.3)
P 		4479.7
(-3)
P 		2856
B 6233.1
(0.3)
P 		5838.4
(-0.3)
P 		8022
(3)
P 		5592.4
(-0.6)
P 		5038.4
(-1.5)
P 		4006.1
(-3)
P 		4016
C 6087.8
(0.8)
P 		5512.9
(-0.2)
P 		7362
(3)
P 		4992.1
(-1.1)
P 		5709
(0.1)
P 		3935
(-3)
P 		3427
avr 6185.5
(0.6) 		5883
(0) 		7573.1
(3) 		5645.6
(-0.4) 		5186.9
(-1.2) 		4140.3
(-3) 		3433
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2160
1 2 3 4 range
A 2447.6
(3)
P 		1287.4
(-0.2)
P 		1686.4
(0.9)
P 		293
(-3)
P 		2155
B 2082.9
(3)
P 		1544.4
(1)
P 		1640.9
(1.4)
P 		441.8
(-3)
P 		1641
C 2194.5
(2.7)
P 		2191.4
(2.7)
P 		2290.9
(3)
P 		287.4
(-3)
P 		2004
avr 2241.7
(3) 		1674.4
(1.2) 		1872.7
(1.8) 		340.7
(-3) 		1901

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1253
1 2 3 4 5 6 range
A 1149.5
(-2.7)
P 		2239.8
(3)
P 		1597.5
(-0.4)
P 		1611.1
(-0.3)
P 		1452.4
(-1.1)
P 		1090.7
(-3)
P 		1149
B 2168.8
(3)
P 		1943.3
(1)
P 		1948
(1.1)
P 		1481.2
(-3)
P 		2047
(1.9)
P 		1493.8
(-2.9)
P 		688
C 2256.5
(3)
P 		1682.1
(0.2)
P 		1873.8
(1.2)
P 		1003.9
(-3)
P 		1847.4
(1)
P 		1317.3
(-1.5)
P 		1253
avr 1858.3
(2.1) 		1955.1
(3) 		1806.4
(1.6) 		1365.4
(-2.4) 		1782.3
(1.4) 		1300.6
(-3) 		655
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191305_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.224
Sequence Type Exemplar sequence
Representative Public ID T03F1.3 NCBI
Target Description T03F1.3 /REP_DB=WormBase Gene ID /WP=CE13100 /TR=SW:P91427 /GB=AAB42230.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=phosphoglycerate kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058844(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058844 NCBI Caenorhabditis elegans T03F1.3 (T03F1.3) mRNA, complete cds. 11/11 None
SNAP00000013118 ENSEMBL cdna:SNAP chromosome:CEL140:I:3867554:3868959:1 11/11 None
GENEFINDER00000013125 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3867554:3868959:1 11/11 None
T03F1.3.2 ENSEMBL cdna:known chromosome:CEL140:I:3867541:3869100:1 gene:T03F1.3 11/11 None
T03F1.3.1 ENSEMBL cdna:known chromosome:CEL140:I:3867543:3869115:1 gene:T03F1.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3867553-3868959(+) 100.0 100.0
Public Domain and Genome References
Gene Title phosphoglycerate kinase
Ensembl T03F1.3
Entrez Gene 171965 Entrez gene
SwissProt P91427 EMBL-EBI
WormBase CE13100 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1598895_AT similar to phosphoglycerate kinase 1 cfa
CANINE:1596036_AT similar to phosphoglycerate kinase 1 cfa
CANINE:1587792_AT similar to phosphoglycerate kinase 1 cfa
CANINE:1585281_S_AT similar to phosphoglycerate kinase 1 cfa
CANINE_2:CFA.1363.1.A1_X_AT similar to phosphoglycerate kinase 1 cfa
CANINE_2:CFAAFFX.16483.1.S1_AT similar to phosphoglycerate kinase 1 cfa
CANINE_2:CFAAFFX.16483.1.S1_S_AT similar to phosphoglycerate kinase 1 cfa
CANINE_2:CFAAFFX.26428.1.S1_S_AT similar to phosphoglycerate kinase 1 cfa
DROSOPHILA_2:1639766_AT Phosphoglycerate kinase dm
DROSGENOME1:143301_AT Phosphoglycerate kinase dm
CHICKEN:GGA.4503.1.S1_A_AT phosphoglycerate kinase 1 gga
HG-U95AV2:37677_AT phosphoglycerate kinase 1 hs
HG-U133_PLUS_2:200738_S_AT phosphoglycerate kinase 1 hs
HG-U133A:200738_S_AT phosphoglycerate kinase 1 hs
HG-FOCUS:200738_S_AT phosphoglycerate kinase 1 hs
HG-U133A_2:200738_S_AT phosphoglycerate kinase 1 hs
HG-U133_PLUS_2:200737_AT phosphoglycerate kinase 1 hs
HG-U133A:200737_AT phosphoglycerate kinase 1 hs
HG-U133A_2:200737_AT phosphoglycerate kinase 1 hs
HG-U133_PLUS_2:227068_AT phosphoglycerate kinase 1 hs
HG-U133B:227068_AT phosphoglycerate kinase 1 hs
HUGENEFL:V00572_AT phosphoglycerate kinase 1 hs
U133_X3P:G4505762_3P_A_AT phosphoglycerate kinase 1 hs
U133_X3P:HS.78771.1.S1_3P_AT phosphoglycerate kinase 1 hs
U133_X3P:HS.169313.0.S1_3P_S_AT phosphoglycerate kinase 1 hs
HG-U133A_2:217356_S_AT phosphoglycerate kinase 1 hs
HG-U133_PLUS_2:217356_S_AT phosphoglycerate kinase 1 hs
HG-U133A:217356_S_AT phosphoglycerate kinase 1 hs
HG-U95AV2:31488_S_AT phosphoglycerate kinase 1 hs
HG-U95E:91445_AT phosphoglycerate kinase 1 hs
HG-U133_PLUS_2:228483_S_AT Phosphoglycerate kinase 1 hs
HG-U133B:228483_S_AT Phosphoglycerate kinase 1 hs
HG-U133_PLUS_2:221617_AT Phosphoglycerate kinase 1 hs
HG-U133A_2:221617_AT Phosphoglycerate kinase 1 hs
HG-FOCUS:221617_AT Phosphoglycerate kinase 1 hs
HG-U133A:221617_AT Phosphoglycerate kinase 1 hs
HUGENEFL:S81916_AT Phosphoglycerate kinase 1 hs
U133_X3P:HS2.288933.1.S1_3P_AT Phosphoglycerate kinase 1 hs
U133_X3P:HS.171723.1.A1_3P_AT Phosphoglycerate kinase 1 hs
U133_X3P:HS.171723.0.A1_3P_AT Phosphoglycerate kinase 1 hs
U133_X3P:HS.169313.0.S1_3P_AT Phosphoglycerate kinase 1 hs
HG-U133_PLUS_2:1558365_AT Phosphoglycerate kinase 1 hs
HG-U133A_2:221616_S_AT Phosphoglycerate kinase 1 hs
HG-U133_PLUS_2:221616_S_AT Phosphoglycerate kinase 1 hs
HG-U133A:221616_S_AT Phosphoglycerate kinase 1 hs
HG-U133A_2:217383_AT Phosphoglycerate kinase 1 hs
HG-U133_PLUS_2:217383_AT Phosphoglycerate kinase 1 hs
HG-U133A:217383_AT Phosphoglycerate kinase 1 hs
HU35KSUBA:D82614_AT Phosphoglycerate kinase 1 hs
MG-U74AV2:93346_AT phosphoglycerate kinase 1 mm
MOE430A:1417864_AT phosphoglycerate kinase 1 mm
MOUSE430_2:1417864_AT phosphoglycerate kinase 1 mm
MOUSE430A_2:1417864_AT phosphoglycerate kinase 1 mm
MU11KSUBA:M15668_S_AT phosphoglycerate kinase 1 mm
MU11KSUBB:MSA.16316.0_F_AT phosphoglycerate kinase 1 mm
MU11KSUBB:MSA.37855.0_S_AT phosphoglycerate kinase 1 mm
MU11KSUBB:MSA.30602.0_F_AT phosphoglycerate kinase 1 mm
MG-U74BV2:164545_F_AT phosphoglycerate kinase 1 mm
MOE430A:1438640_X_AT phosphoglycerate kinase 1 mm
MOUSE430A_2:1438640_X_AT phosphoglycerate kinase 1 mm
MOUSE430_2:1438640_X_AT phosphoglycerate kinase 1 mm
MOE430A:1439435_X_AT phosphoglycerate kinase 1 mm
MOUSE430A_2:1439435_X_AT phosphoglycerate kinase 1 mm
MOUSE430_2:1439435_X_AT phosphoglycerate kinase 1 mm
MOE430A:1417864_AT similar to Pgk1 protein mm
MOUSE430_2:1417864_AT similar to Pgk1 protein mm
MOUSE430A_2:1417864_AT similar to Pgk1 protein mm
MU11KSUBA:M15668_S_AT similar to Pgk1 protein mm
MU11KSUBB:MSA.16316.0_F_AT similar to Pgk1 protein mm
MU11KSUBB:MSA.37855.0_S_AT similar to Pgk1 protein mm
MU11KSUBB:MSA.30602.0_F_AT similar to Pgk1 protein mm
MG-U74BV2:164545_F_AT similar to Pgk1 protein mm
MOE430A:1438640_X_AT similar to Pgk1 protein mm
MOUSE430A_2:1438640_X_AT similar to Pgk1 protein mm
MOUSE430_2:1438640_X_AT similar to Pgk1 protein mm
MOE430A:1439435_X_AT similar to Pgk1 protein mm
MOUSE430A_2:1439435_X_AT similar to Pgk1 protein mm
MOUSE430_2:1439435_X_AT similar to Pgk1 protein mm
MU19KSUBC:TC35842_AT PREDICTED: similar to Pgk1 protein [Mus musculus], mRNA sequence mm
RG-U34A:M31788_AT phosphoglycerate kinase 1 rn
RG-U34A:M31788_G_AT phosphoglycerate kinase 1 rn
RG-U34A:RC_AA892797_AT phosphoglycerate kinase 1 rn
RAE230A:1388318_AT phosphoglycerate kinase 1 rn
RAT230_2:1388318_AT phosphoglycerate kinase 1 rn
RAE230A:1368906_AT phosphoglycerate kinase 1 rn
RAT230_2:1368906_AT phosphoglycerate kinase 1 rn
RAE230A:1387361_S_AT phosphoglycerate kinase 1 rn
RAT230_2:1387361_S_AT phosphoglycerate kinase 1 rn
YEAST_2:1772010_AT 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6096 glycolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4618 phosphoglycerate kinase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_491245 T03F1.3 [Caenorhabditis elegans] gb|AAB42230.1| Hypothetical protein T03F1.3 [Caenorhabditis elegans] sp|P91427|PGK_CAEEL Probable phosphoglycerate kinase 0.0
blast CAE66637 Hypothetical protein CBG11974 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001576 EMBL-EBI Phosphoglycerate kinase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:191305_AT
gaggaatggcctataccttcttgaaagttgcccagggagtcaagatcggaaactctttgt
acgacgaagaaggagcgaaaattgtgaacgagcttcttgaggctgccaaggcaaagggag
ttcaaatccatctcccagtggatttcgtcattgctgacaagttcgccgaggacgcaacct
cgaagaccgtcaccgccgaggaaggtgttccagatggccacatgggacttgatgttgggc
cggaatcttctaagatcttcgccgctgccattcaacgcgccaaaacgattgtctggaacg
gtccagcaggagtattcgagtttgataaattcgccacaggcaccaagtcattgatggatg
aagttgtcaaggccaccgctgccggagccatcacaatcattggaggaggtgacactgcga
ccgccgccaagaagtataacacggaagataaggtgtctcacgtctcaactggaggtgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGGAATGGCCTATACCTTCTTGAA 394 405 722 Antisense
TCCATCTCCCAGTGGATTTCGTCAT 352 591 848 Antisense
CGTCATTGCTGACAAGTTCGCCGAG 553 245 867 Antisense
AGACCGTCACCGCCGAGGAAGGTGT 8 71 905 Antisense
GGTGTTCCAGATGGCCACATGGGAC 242 499 925 Antisense
GGAATCTTCTAAGATCTTCGCCGCT 162 533 963 Antisense
TCGCCACAGGCACCAAGTCATTGAT 290 599 1052 Antisense
GGATGAAGTTGTCAAGGCCACCGCT 105 515 1077 Antisense
CTGCCGGAGCCATCACAATCATTGG 396 237 1100 Antisense
TGCGACCGCCGCCAAGAAGTATAAC 71 583 1137 Antisense
GTCTCACGTCTCAACTGGAGGTGGA 551 469 1176 Antisense
Sequence Source Affymetrix Proprietary Database