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[191294_at] cystathionine beta-synthase

Description ZC373.1 /REP_DB=WormBase Gene ID /WP=CE25661 /TR=Q23264 /GB=CAA88980.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=cystathionine beta-synthase
Cluster 6grp_highest_10_10 > 53      6grp_high1_10_10 > 35      4grp_highest_8_8 > 15      4grp_high_8_8 > 14      6grp_highest_nonorm > 31      6grp_total > 35      4grp_total > 09      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1951
1 2 3 4 range
A 1674.4
(-3)
P 		2172
(-1.2)
P 		2005.4
(-1.8)
P 		3314.7
(3)
P 		1640
B 2192.1
(-2.8)
P 		2135.8
(-3)
P 		2160.4
(-2.9)
P 		3625.2
(3)
P 		1489
C 1716.2
(-3)
P 		2188
(-1.4)
P 		2049.3
(-1.9)
P 		3493.3
(3)
P 		1777
avr 1860.9
(-3) 		2165.3
(-1.9) 		2071.7
(-2.2) 		3477.7
(3) 		1617

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1081
1 2 3 4 5 6 range
A 1254.3
(2.3)
P 		1367.4
(3)
P 		974
(0.7)
P 		632.6
(-1.3)
P 		352.9
(-3)
P 		803
(-0.3)
P 		1015
B 1309.6
(2.4)
P 		1422.8
(3)
P 		968.4
(0.5)
P 		662.6
(-1.2)
P 		347.6
(-3)
P 		782.7
(-0.6)
P 		1075
C 1343
(2.7)
P 		1392.9
(3)
P 		1152.9
(1.6)
P 		733.7
(-0.8)
P 		341.4
(-3)
P 		1033.6
(0.9)
P 		1052
avr 1302.3
(2.5) 		1394.4
(3) 		1031.8
(0.9) 		676.3
(-1.1) 		347.3
(-3) 		873.1
(0) 		1047
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 301
1 2 3 4 range
A 609.6
(3)
P 		471.5
(-3)
P 		524.2
(-0.7)
P 		485.2
(-2.4)
P 		138
B 699
(1.4)
P 		527.5
(-2.4)
P 		500.6
(-3)
P 		772.5
(3)
P 		272
C 568.1
(-3)
P 		629.1
(-0.5)
P 		714.2
(3)
P 		589
(-2.1)
P 		146
avr 625.6
(3) 		542.7
(-3) 		579.7
(-0.3) 		615.6
(2.3) 		83

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 391
1 2 3 4 5 6 range
A 231.2
(-1)
P 		486.3
(3)
P 		212.1
(-1.3)
P 		160.4
(-2.1)
P 		106.5
(-3)
P 		195.5
(-1.6)
P 		380
B 455.7
(2.7)
P 		473.6
(3)
P 		235.2
(-1.3)
P 		175.5
(-2.4)
P 		141.2
(-3)
P 		291.9
(-0.3)
P 		332
C 497.8
(3)
P 		425
(1.9)
P 		293.4
(-0.2)
P 		147.6
(-2.4)
P 		110.5
(-3)
P 		346
(0.6)
P 		387
avr 394.9
(1.8) 		461.6
(3) 		246.9
(-0.8) 		161.2
(-2.3) 		119.4
(-3) 		277.8
(-0.2) 		342
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191294_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16740
Sequence Type Exemplar sequence
Representative Public ID ZC373.1 NCBI
Target Description ZC373.1 /REP_DB=WormBase Gene ID /WP=CE25661 /TR=Q23264 /GB=CAA88980.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=cystathionine beta-synthase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077285(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077285 NCBI Caenorhabditis elegans ZC373.1 (ZC373.1) mRNA, complete cds. 11/11 None
SNAP00000017661 ENSEMBL cdna:SNAP chromosome:CEL140:X:10054613:10057308:1 11/11 None
GENEFINDER00000017669 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:10054613:10057308:1 11/11 None
ZC373.1.2 ENSEMBL cdna:known chromosome:CEL140:X:10054206:10057445:1 gene:ZC373.1 11/11 None
ZC373.1.1 ENSEMBL cdna:known chromosome:CEL140:X:10054211:10057445:1 gene:ZC373.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:10054422-10057308(+) 100.0 100.0
Public Domain and Genome References
Gene Title cystathionine beta-synthase
Ensembl ZC373.1
Entrez Gene 181215 Entrez gene
SwissProt Q23264 EMBL-EBI
WormBase CE31670 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6520 amino acid metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16829 lyase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB63415 Hypothetical protein ZC373.1 [Caenorhabditis elegans] emb|CAA88980.3| Hypothetical protein ZC373.1 [Caenorhabditis elegans] ref|NP_509686.2| ZC373.1 [Caenorhabditis elegans] 0.0
blast CAE58747 Hypothetical protein CBG01937 [Caenorhabditis briggsae] 0.0
blast AAF35961 Hypothetical protein F54A3.4 [Caenorhabditis elegans] ref|NP_494215.1| F54A3.4 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001926 EMBL-EBI Pyridoxal-5-phosphate-dependent enzyme, beta subunit 6.2E-35
Pfam IPR001926 EMBL-EBI Pyridoxal-5-phosphate-dependent enzyme, beta subunit 1.8E-20
Pfam IPR001926 EMBL-EBI Pyridoxal-5-phosphate-dependent enzyme, beta subunit 5.9E-108
Pfam IPR001926 EMBL-EBI Pyridoxal-5-phosphate-dependent enzyme, beta subunit 6.2E-35
Pfam IPR001926 EMBL-EBI Pyridoxal-5-phosphate-dependent enzyme, beta subunit 1.8E-20
Pfam IPR001926 EMBL-EBI Pyridoxal-5-phosphate-dependent enzyme, beta subunit 5.9E-108
Sequence
Target Sequence 
>C. ELEGANS:191294_AT
gacagcccacattcacacattggtgttgctctccgtcttaaaagtgaaattcaagatgcg
gtagttcttgatcaatactgcaatccaggaaatccgttagctcattatgaagaaaccgct
gaggaaatcatttacgatatgggggataagcatattgatctcgtggttctcactgctggt
acaggaggaactgttactggaatttctcgcaaaatccacgagcgtattccaacagccaag
gtggtcggagtcgaccctcatggatctattctcgctggacctgccgagactgacatcgac
ttctatgaggttgaaggaattggatacgattttttgccaggaactttggacactagtgcc
attgactattgggcaaagtctcatgacaaggagtcgttcttgatggctcgcgagctcatc
agatctgagggtattttgtgcggaggatcttccggttgcgcggttcactacgcacttgaa
gaatgcaaatcgttgaatcttccagccgaggctaatgtcgttgttcttcttcccgatgga
attagaaattacatcactaaattcctcgacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAGCCCACATTCACACATTGGTG 385 367 1507 Antisense
TGTTGCTCTCCGTCTTAAAAGTGAA 493 559 1530 Antisense
GGTTCTCACTGCTGGTACAGGAGGA 448 505 1671 Antisense
ACGAGCGTATTCCAACAGCCAAGGT 264 93 1724 Antisense
AAGGTGGTCGGAGTCGACCCTCATG 159 159 1744 Antisense
TGCCGAGACTGACATCGACTTCTAT 561 585 1788 Antisense
GTTCTTGATGGCTCGCGAGCTCATC 103 445 1902 Antisense
TTGTGCGGAGGATCTTCCGGTTGCG 475 705 1942 Antisense
TTGCGCGGTTCACTACGCACTTGAA 653 699 1962 Antisense
GCCGAGGCTAATGTCGTTGTTCTTC 247 285 2011 Antisense
ATTACATCACTAAATTCCTCGACGA 175 13 2054 Antisense
Sequence Source Affymetrix Proprietary Database