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[191293_at] 7tm receptor

Description T04A8.1 /REP_DB=WormBase Gene ID /WP=CE01064 /GEN=srg-10 /TR=SW:P46568 /GB=CAA84725.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=7tm receptor
Cluster 6grp_high1_10_10 > 70      6grp_total > 03      4grp_total > 32      6grp_cgc > 40      6grp_high1_sub > 04      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 37.2
(-3)
A 		39.9
(-2.6)
A 		79.9
(3)
P 		58.4
(-0)
A 		43
B 29
(-3)
A 		32.6
(-2.4)
A 		67.8
(3)
M 		59.4
(1.7)
P 		39
C 52
(-2.9)
A 		54
(-2.3)
A 		51.7
(-3)
P 		70.6
(3)
P 		19
avr 39.4
(-3) 		42.2
(-2.4) 		66.5
(3) 		62.8
(2.2) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 97
1 2 3 4 5 6 range
A 54.5
(3)
P 		54.1
(2.9)
P 		29.9
(-2.8)
A 		28.9
(-3)
A 		47.1
(1.3)
P 		50
(1.9)
P 		26
B 71.6
(2)
P 		81.7
(3)
P 		44.4
(-0.7)
A 		67.4
(1.6)
M 		21.7
(-3)
P 		43.2
(-0.9)
A 		60
C 62.5
(0.3)
P 		67.2
(0.6)
P 		32.4
(-1.5)
P 		105.5
(3)
P 		56
(-0.1)
A 		8.3
(-3)
A 		97
avr 62.9
(2.2) 		67.7
(3) 		35.6
(-2.7) 		67.3
(2.9) 		41.6
(-1.6) 		33.8
(-3) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 13.5
(-0.6)
A 		8.7
(-3)
A 		20.9
(3)
P 		8.6
(-3)
A 		12
B 9.3
(-2)
A 		8
(-3)
A 		15.7
(3)
M 		12.7
(0.7)
P 		8
C 17.2
(2.2)
A 		15.5
(0.5)
A 		18
(3)
P 		11.9
(-3)
P 		6
avr 13.3
(-0.9) 		10.7
(-3) 		18.2
(3) 		11.1
(-2.7) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 10.1
(-1.3)
P 		19.2
(3)
P 		6.5
(-3)
A 		7.3
(-2.6)
A 		14.2
(0.6)
P 		12.2
(-0.3)
P 		13
B 24.9
(2.3)
P 		27.2
(3)
P 		10.8
(-2.3)
A 		17.8
(-0.1)
M 		8.8
(-3)
P 		16.1
(-0.6)
A 		18
C 23.2
(3)
P 		20.5
(2.2)
P 		8.2
(-1.4)
P 		21.2
(2.4)
P 		18.1
(1.5)
A 		2.8
(-3)
A 		20
avr 19.4
(1.7) 		22.3
(3) 		8.5
(-3) 		15.4
(0) 		13.7
(-0.7) 		10.4
(-2.2) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191293_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7991
Sequence Type Exemplar sequence
Representative Public ID T04A8.1 NCBI
Target Description T04A8.1 /REP_DB=WormBase Gene ID /WP=CE01064 /GEN=srg-10 /TR=SW:P46568 /GB=CAA84725.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=7tm receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065553(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065553 NCBI Caenorhabditis elegans Serpentine Receptor, class G (gamma) family member (srg-10) (srg-10) mRNA, complete cds. 11/11 None
SNAP00000041089 ENSEMBL cdna:SNAP chromosome:CEL140:III:4677727:4679626:1 11/11 None
GENEFINDER00000041109 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4677729:4679785:1 9/11 None
T04A8.1 ENSEMBL cdna:known chromosome:CEL140:III:4677729:4679626:1 gene:T04A8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4677728-4679626(+) 100.0 100.0
Public Domain and Genome References
Gene Title 7tm receptor
Gene Symbol srg-10
Ensembl T04A8.1
Entrez Gene 191836 Entrez gene
SwissProt P46568 EMBL-EBI
WormBase CE01064 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7165 signal transduction inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4888 transmembrane receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA84725 Hypothetical protein T04A8.1 [Caenorhabditis elegans] ref|NP_497954.1| Serpentine Receptor, class G (gamma) family member (srg-10) [Caenorhabditis elegans] sp|P46568|SRG10_CAEEL Serpentine receptor class gamma-10 (Protein srg-10) 0.0
blast 2123261AK chemosensory receptor 0.0
blast CAA84725 Hypothetical protein T04A8.1 [Caenorhabditis elegans] ref|NP_497954.1| Serpentine Receptor, class G (gamma) family member (srg-10) [Caenorhabditis elegans] sp|P46568|SRG10_CAEEL Serpentine receptor class gamma-10 (Protein srg-10) 1.0E-155
blast 2123261AK chemosensory receptor 1.0E-152
blast CAE71164 Hypothetical protein CBG18020 [Caenorhabditis briggsae] 1.0E-134
Protein Domains
Database ID Description E-Value
Pfam IPR000609 EMBL-EBI Integral membrane protein Srg, nematode type 1.0E-126
Pfam IPR000609 EMBL-EBI Integral membrane protein Srg, nematode type 1.0E-126
Pfam IPR000609 EMBL-EBI Integral membrane protein Srg, nematode type 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497954.1 8 40-57,67-89,91-113,128-146,159-181,201-223,243-265,275-297
T04A8.1 8 40-57,67-89,91-113,128-146,159-181,201-223,243-265,275-297
SNAP00000041089 8 41-58,68-90,92-114,129-147,160-182,202-224,244-266,276-298
GENEFINDER00000041109 8 40-57,67-89,91-113,128-146,159-181,201-223,243-265,280-302
Sequence
Target Sequence 
>C. ELEGANS:191293_AT
gatgacctgtgttattttcctaatgaagcatttgaagctatggaaacaaatattgaagcc
tgtcttaattattacattcatcctcccgcttggtgtgatctggaaaattttgttatcccg
tgtgtacatcaacccaaacggagctggattttcggtgaactacaaagattatttcccatg
ggcgaatatttcaattcttcatctgttccacttcacactttgctttgtactagttatcat
attctttgtcgcaacaattcttggtctgacaatgctgaaacaaagaattaagagtgctga
aagatcattgacaattgttacgatgattatggcagtgcagacagtgactttcgcatctat
tcagatctactttgtctttttcgcggcctacactccaaagatccgaagtgtccttctaca
aattgtgtcatttgtttttgattctctttatgtattcagtccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATGACCTGTGTTATTTTCCTAATG 556 409 426 Antisense
CTTAATTATTACATTCATCCTCCCG 439 221 489 Antisense
CCTCCCGCTTGGTGTGATCTGGAAA 511 267 507 Antisense
TGTTATCCCGTGTGTACATCAACCC 11 561 536 Antisense
AACGGAGCTGGATTTTCGGTGAACT 631 143 562 Antisense
ATTTCAATTCTTCATCTGTTCCACT 588 15 613 Antisense
TCCACTTCACACTTTGCTTTGTACT 130 589 632 Antisense
GTGCAGACAGTGACTTTCGCATCTA 384 481 760 Antisense
CAGATCTACTTTGTCTTTTTCGCGG 678 205 787 Antisense
CGGCCTACACTCCAAAGATCCGAAG 664 241 809 Antisense
GATTCTCTTTATGTATTCAGTCCAA 595 431 865 Antisense
Sequence Source Affymetrix Proprietary Database