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[191289_s_at] 7TM chemoreceptor, srh family

Description C32B5.2 /REP_DB=WormBase Gene ID /WP=CE08510 /GEN=srh-274 /TR=P91118 /GB=AAB37958.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster 6grp_total > 70      4grp_total > 10      6grp_cgc > 95      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 139
1 2 3 4 range
A 25.1
(-1.5)
A 		7.5
(-3)
A 		12.1
(-2.6)
A 		76.3
(3)
A 		69
B 22.9
(-3)
A 		37.5
(3)
A 		35.2
(2.1)
A 		31.6
(0.6)
A 		15
C 4.6
(-3)
A 		35
(-1.7)
A 		10.9
(-2.7)
A 		143.3
(3)
P 		139
avr 17.5
(-3) 		26.7
(-2.2) 		19.4
(-2.8) 		83.7
(3) 		66

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 11.1
(0.2)
A 		19.4
(3)
A 		18.9
(2.8)
A 		18.4
(2.7)
A 		1.7
(-3)
A 		7.4
(-1.1)
A 		18
B 18.9
(-0.6)
A 		9.3
(-2)
A 		9.9
(-1.9)
A 		43.5
(3)
A 		3
(-3)
A 		2.7
(-3)
A 		41
C 6.3
(-3)
A 		38.8
(2.2)
A 		21.5
(-0.6)
A 		43.8
(3)
A 		19
(-1)
A 		26.1
(0.2)
A 		38
avr 12.1
(-2.1) 		22.5
(0.2) 		16.8
(-1) 		35.2
(3) 		7.9
(-3) 		12.1
(-2.1) 		27
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 9.1
(1.7)
A 		1.6
(-3)
A 		3.2
(-2)
A 		11.2
(3)
A 		10
B 7.3
(-1.6)
A 		9.3
(3)
A 		8.2
(0.5)
A 		6.7
(-3)
A 		3
C 1.5
(-3)
A 		10.1
(-0.7)
A 		3.8
(-2.4)
A 		24.2
(3)
P 		23
avr 6
(-2.4) 		7
(-1.7) 		5.1
(-3) 		14
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 2
(-1.6)
A 		6.9
(3)
A 		4.1
(0.4)
A 		4.7
(0.9)
A 		0.5
(-3)
A 		1.8
(-1.8)
A 		6
B 6.6
(0.2)
A 		3.1
(-1.8)
A 		2.4
(-2.2)
A 		11.5
(3)
A 		1.2
(-2.9)
A 		1
(-3)
A 		11
C 2.3
(-3)
A 		11.8
(3)
A 		5.5
(-1)
A 		8.8
(1.1)
A 		6.1
(-0.6)
A 		8.7
(1)
A 		10
avr 3.6
(-1.9) 		7.3
(1.9) 		4
(-1.5) 		8.3
(3) 		2.6
(-3) 		3.8
(-1.7) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191289_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4869
Sequence Type Exemplar sequence
Representative Public ID C32B5.2 NCBI
Target Description C32B5.2 /REP_DB=WormBase Gene ID /WP=CE08510 /GEN=srh-274 /TR=P91118 /GB=AAB37958.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061473(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061473 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-274) (srh-274) mRNA, complete cds. 11/11 A 
GENEFINDER00000023033 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:1054102:1054584:-1 10/11 None
GENEFINDER00000042185 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:981288:984570:1 11/11 None
C32B5.2 ENSEMBL cdna:known chromosome:CEL140:II:983148:984570:1 gene:C32B5.2 11/11 A 
K02E7.8 ENSEMBL cdna:pseudogene chromosome:CEL140:II:1054102:1054693:-1 gene:K02E7.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:983146-984569(+) 100.0 100.0
chrII:1054100-1054690(-) 53.66 53.66
Public Domain and Genome References
Gene Title 7TM chemoreceptor, srh family
Gene Symbol srh-274
Ensembl C32B5.2
K02E7.8
Entrez Gene 183106 Entrez gene
SwissProt P91118 EMBL-EBI
WormBase CE08510 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_493874 Serpentine Receptor, class H family member (srh-274) [Caenorhabditis elegans] gb|AAB37958.1| Serpentine receptor, class h protein 274 [Caenorhabditis elegans] 1.0E-167
blast NP_493874 Serpentine Receptor, class H family member (srh-274) [Caenorhabditis elegans] gb|AAB37958.1| Serpentine receptor, class h protein 274 [Caenorhabditis elegans] 1.0E-166
blast NP_493873 C32B5.3 [Caenorhabditis elegans] gb|AAB37959.1| Hypothetical protein C32B5.3 [Caenorhabditis elegans] 1.0E-109
blast AAB52307 Serpentine receptor, class h protein 275 [Caenorhabditis elegans] ref|NP_504876.1| Serpentine Receptor, class H family member (srh-275) [Caenorhabditis elegans] 1.0E-101
blast AAB52307 Serpentine receptor, class h protein 275 [Caenorhabditis elegans] ref|NP_504876.1| Serpentine Receptor, class H family member (srh-275) [Caenorhabditis elegans] 1.0E-100
blast T32387 hypothetical protein K02E7.8 - Caenorhabditis elegans 2.0E-83
blast NP_493874 Serpentine Receptor, class H family member (srh-274) [Caenorhabditis elegans] gb|AAB37958.1| Serpentine receptor, class h protein 274 [Caenorhabditis elegans] 6.0E-75
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 3.2E-67
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_493874.1 7 15-37,50-72,92-114,134-156,190-212,240-262,277-296
C32B5.2 7 15-37,50-72,92-114,134-156,190-212,240-262,277-296
GENEFINDER00000023033 3 23-45,73-95,110-129
GENEFINDER00000042185 7 304-326,339-361,381-403,423-445,479-501,529-551,566-585
Sequence
Target Sequence 
>C. ELEGANS:191289_S_AT
acaggcgctgcaggaggtttttgagatgctccccgagctccccaaagagctccaaacctc
aaaaatcttcgttctagccacagatttccggtttatagtactacccgtcttttttatgtc
gaccctattattgctggaatccggaattttcacatttttgatctacaaaaatatgagcgc
ccaaagcaaggagctctccctatccccacaaactataaaaatgcaaaagcagttcctacg
agccctgaacatccaaacctgcatcccacttgccatactcttgatcccactgtgctattt
ggtgatttcccgggttttcctgatctacaaccaagttgctaacaatttttgttttataac
catcggagcccacggcctgttttccacgcttatcatgctctatatacatattccgtatcg
aaatttctgtgtggataagttgtgctcaaaggtacctggcttcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAGGCGCTGCAGGAGGTTTTTGAG 261 107 489 Antisense
ACCTCAAAAATCTTCGTTCTAGCCA 564 85 544 Antisense
TCTAGCCACAGATTTCCGGTTTATA 172 615 561 Antisense
GTCGACCCTATTATTGCTGGAATCC 332 473 606 Antisense
CCAAAGCAAGGAGCTCTCCCTATCC 473 275 669 Antisense
TGCAAAAGCAGTTCCTACGAGCCCT 397 575 710 Antisense
CTCTTGATCCCACTGTGCTATTTGG 487 229 766 Antisense
GTGCTATTTGGTGATTTCCCGGGTT 622 479 780 Antisense
TGTTTTATAACCATCGGAGCCCACG 276 559 838 Antisense
TTCCACGCTTATCATGCTCTATATA 582 699 870 Antisense
TGTGCTCAAAGGTACCTGGCTTCAA 577 553 929 Antisense
Sequence Source Affymetrix Proprietary Database