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[191284_at] Human PHP32 protein like

Description K11D2.2 /REP_DB=WormBase Gene ID /WP=CE12120 /TR=SW:O45686 /GB=CAB05556.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Human PHP32 protein like
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 21      4grp_highest_8_8 > 41      4grp_high_8_8 > 42      6grp_highest_nonorm > 05      6grp_total > 32      4grp_total > 53      6grp_highest_sub > De      6grp_cgc > 70      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6202
1 2 3 4 range
A 6996.1
(3)
P 		5075.5
(1.1)
P 		3385.9
(-0.5)
P 		794.2
(-3)
P 		6202
B 6071.2
(3)
P 		3622.5
(0.1)
P 		3437.6
(-0.1)
P 		958.8
(-3)
P 		5112
C 6267
(3)
P 		3881.4
(0.4)
P 		3153.1
(-0.4)
P 		829
(-3)
P 		5438
avr 6444.8
(3) 		4193.1
(0.6) 		3325.5
(-0.4) 		860.7
(-3) 		5584

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7601
1 2 3 4 5 6 range
A 6812.2
(-0)
P 		9490.7
(3)
P 		7857.7
(1.1)
P 		7480.6
(0.7)
P 		4922.5
(-2.2)
P 		4199
(-3)
P 		5292
B 6441.2
(1.2)
P 		6713.9
(1.5)
P 		8017.6
(3)
P 		5015.4
(-0.3)
P 		5765.9
(0.5)
P 		2630.5
(-3)
P 		5387
C 8865.3
(1.9)
P 		6058.3
(-0.3)
P 		10223.9
(3)
P 		5905.9
(-0.4)
P 		7724.4
(1)
P 		2623.3
(-3)
P 		7601
avr 7372.9
(1.6) 		7421
(1.6) 		8699.7
(3) 		6134
(0.2) 		6137.6
(0.2) 		3150.9
(-3) 		5549
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2431
1 2 3 4 range
A 2547.3
(3)
P 		1101.8
(-0.6)
P 		885.1
(-1.1)
P 		116.3
(-3)
P 		2431
B 1936
(3)
P 		894.7
(-0.6)
P 		796.6
(-0.9)
P 		204.3
(-3)
P 		1732
C 2074.6
(3)
P 		1115.9
(0)
P 		1099
(-0)
P 		139.8
(-3)
P 		1935
avr 2186
(3) 		1037.5
(-0.4) 		926.9
(-0.7) 		153.5
(-3) 		2033

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2497
1 2 3 4 5 6 range
A 1255.8
(-2.4)
P 		3375.4
(3)
P 		1711.2
(-1.2)
P 		1897.3
(-0.8)
P 		1485.4
(-1.8)
P 		1022.3
(-3)
P 		2353
B 2241.2
(2.6)
P 		2234.8
(2.5)
P 		1946.9
(1.3)
P 		1328.4
(-1.5)
P 		2342.6
(3)
P 		980.9
(-3)
P 		1362
C 3286
(3)
P 		1848.6
(-0.6)
P 		2602.2
(1.3)
P 		1187.7
(-2.2)
P 		2499.5
(1)
P 		878.2
(-3)
P 		2408
avr 2261
(2.1) 		2486.3
(3) 		2086.8
(1.4) 		1471.1
(-1) 		2109.2
(1.5) 		960.5
(-3) 		1526
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191284_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2203
Sequence Type Exemplar sequence
Representative Public ID K11D2.2 NCBI
Target Description K11D2.2 /REP_DB=WormBase Gene ID /WP=CE12120 /TR=SW:O45686 /GB=CAB05556.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Human PHP32 protein like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060772(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060772 NCBI Caenorhabditis elegans AcylSphingosine AmidoHydrolase family member (asah-1) (asah-1) mRNA, complete cds. 11/11 None
SNAP00000019736 ENSEMBL cdna:SNAP chromosome:CEL140:I:12513246:12515632:1 11/11 None
GENEFINDER00000019740 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:12513246:12515632:1 11/11 None
K11D2.2 ENSEMBL cdna:known chromosome:CEL140:I:12513223:12515697:1 gene:K11D2.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12513176-12515563(+) 100.0 100.0
Public Domain and Genome References
Gene Title Human PHP32 protein like
Gene Symbol asah-1
Ensembl K11D2.2
Entrez Gene 173120 Entrez gene
SwissProt O45686 EMBL-EBI
WormBase CE12120 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604008_AT similar to N-acylsphingosine amidohydrolase (acid ceramidase) 1 preproprotein isoform a cfa
CANINE:1604273_AT similar to N-acylsphingosine amidohydrolase (acid ceramidase) 1 preproprotein isoform a cfa
CANINE:1594997_S_AT similar to N-acylsphingosine amidohydrolase (acid ceramidase) 1 preproprotein isoform a cfa
CANINE:1594996_AT similar to N-acylsphingosine amidohydrolase (acid ceramidase) 1 preproprotein isoform a cfa
CANINE_2:CFA.1326.1.S1_AT similar to N-acylsphingosine amidohydrolase (acid ceramidase) 1 preproprotein isoform a cfa
CANINE_2:CFAAFFX.11652.1.S1_S_AT similar to N-acylsphingosine amidohydrolase (acid ceramidase) 1 preproprotein isoform a cfa
CHICKEN:GGA.17521.1.S1_S_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 gga
HG-U133_PLUS_2:213702_X_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HG-U133A_2:213702_X_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HG-U133A:213702_X_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HG-U95AV2:461_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HC-G110:461_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HG-U133_PLUS_2:210980_S_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HG-U133A:210980_S_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HG-FOCUS:210980_S_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HG-U133A_2:210980_S_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HG-U133_PLUS_2:210979_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HG-U133A:210979_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HG-U133A_2:210979_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HU35KSUBB:RC_R60795_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HUGENEFL:U70063_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
U133_X3P:G3860239_3P_X_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
U133_X3P:HS2.75811.3.S1_3P_S_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
U133_X3P:HS.75811.3.S1_3P_A_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
U133_X3P:HS.75811.2.S1_3P_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HG-U133_PLUS_2:1555419_A_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HG-U133A_2:213902_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HG-U133_PLUS_2:213902_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HG-U133A:213902_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HG-U95AV2:36938_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HG-U95C:54993_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
HU35KSUBC:RC_N48707_AT N-acylsphingosine amidohydrolase (acid ceramidase) 1 hs
MG-U74AV2:94282_AT N-acylsphingosine amidohydrolase 1 mm
MG-U74BV2:164648_R_AT N-acylsphingosine amidohydrolase 1 mm
MOE430A:1416735_AT N-acylsphingosine amidohydrolase 1 mm
MOUSE430A_2:1416735_AT N-acylsphingosine amidohydrolase 1 mm
MOUSE430_2:1416735_AT N-acylsphingosine amidohydrolase 1 mm
MU19KSUBB:TC27266_AT Acid ceramidase (Asah1) mm
MU19KSUBB:TC29647_AT Acid ceramidase (Asah1) mm
MU19KSUBB:TC32345_S_AT Acid ceramidase (Asah1) mm
MU19KSUBB:TC32346_AT Acid ceramidase (Asah1) mm
MU19KSUBC:TC41828_AT Acid ceramidase (Asah1) mm
RG-U34A:RC_AA800062_AT N-acylsphingosine amidohydrolase 1 rn
RAE230A:1374770_AT N-acylsphingosine amidohydrolase 1 rn
RAT230_2:1374770_AT N-acylsphingosine amidohydrolase 1 rn
RAE230A:1369077_AT N-acylsphingosine amidohydrolase 1 rn
RAT230_2:1369077_AT N-acylsphingosine amidohydrolase 1 rn
Protein Similarities
Method ID Description E-Value
blast CAB05556 Hypothetical protein K11D2.2 [Caenorhabditis elegans] ref|NP_493173.1| AcylSphingosine AmidoHydrolase family member (asah-1) [Caenorhabditis elegans] sp|O45686|YEC2_CAEEL Hypothetical protein K11D2.2 in chromosome I precursor 0.0
blast CAE69316 Hypothetical protein CBG15381 [Caenorhabditis briggsae] 0.0
blast CAE69317 Hypothetical protein CBG15382 [Caenorhabditis briggsae] 1.0E-164
Protein Domains
Database ID Description E-Value
Pfam IPR003199 EMBL-EBI Choloylglycine hydrolase 4.1E-17
Pfam IPR003199 EMBL-EBI Choloylglycine hydrolase 3.1E-6
Sequence
Target Sequence 
>C. ELEGANS:191284_AT
gtcgaacaactttgccggatacattggaatctacaacggtctcaagccaaacgccttctc
cttgactgctgacgatcgattccagcttgtcggaggatactacggtattctcaagtgggt
gttcggattggaggccgacggaaaatggatgtcctggttggcaagagagaccctcgagac
taagaccacctacctcgacgccaaggagcacttgatgaacactccaatgctctcaccagt
ctacttcattctcggaggatccaagaaggacgagggatgcatcatcgctagaagtctcga
caagaccgcacttctcaccgagatggccacatccccacacggatggtacctgttggagac
caactacgatcagggaaccgaggacctctacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCGAACAACTTTGCCGGATACATT 551 473 603 Antisense
CTACAACGGTCTCAAGCCAAACGCC 571 223 633 Antisense
CCTTGACTGCTGACGATCGATTCCA 710 269 662 Antisense
GATCGATTCCAGCTTGTCGGAGGAT 494 417 676 Antisense
CTCGAGACTAAGACCACCTACCTCG 43 213 775 Antisense
TTGATGAACACTCCAATGCTCTCAC 40 707 814 Antisense
CAGTCTACTTCATTCTCGGAGGATC 521 207 839 Antisense
GGATGCATCATCGCTAGAAGTCTCG 403 513 877 Antisense
GAAGTCTCGACAAGACCGCACTTCT 695 335 893 Antisense
CCCACACGGATGGTACCTGTTGGAG 82 257 936 Antisense
GATCAGGGAACCGAGGACCTCTACC 459 419 970 Antisense
Sequence Source Affymetrix Proprietary Database