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[191281_s_at] alpha tubulin

Description C44B11.3 /REP_DB=WormBase Gene ID /WP=CE24843 /GEN=mec-12 /TR=O01907 /GB=AAB54263.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=alpha tubulin
Cluster 6grp_highest_10_10 > 42      6grp_high1_10_10 > 04      4grp_highest_8_8 > 32      4grp_high_8_8 > 51      6grp_highest_nonorm > 31      6grp_total > 25      4grp_total > 52      6grp_highest_sub > 02      6grp_cgc > 33      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1695
1 2 3 4 range
A 1829.1
(3)
P 		1831.1
(3)
P 		1566.8
(1.9)
P 		381.6
(-3)
P 		1450
B 1913.4
(3)
P 		1893.1
(2.9)
P 		1561.2
(1.7)
P 		339
(-3)
P 		1574
C 1980.9
(3)
P 		1590.7
(1.6)
P 		1346.9
(0.8)
P 		286.1
(-3)
P 		1695
avr 1907.8
(3) 		1771.6
(2.5) 		1491.6
(1.4) 		335.6
(-3) 		1572

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1229
1 2 3 4 5 6 range
A 1240.8
(1.3)
P 		1569.3
(3)
P 		709.9
(-1.5)
P 		613.4
(-2)
P 		412.8
(-3)
P 		492.3
(-2.6)
P 		1157
B 1274.4
(2.5)
P 		1349.1
(3)
P 		874.8
(0.1)
P 		562.6
(-1.8)
P 		433.1
(-2.6)
P 		365.4
(-3)
P 		984
C 1487.4
(3)
P 		1316
(2.1)
P 		1116
(1.1)
P 		527.1
(-2)
P 		678
(-1.2)
P 		340.8
(-3)
P 		1147
avr 1334.2
(2.5) 		1411.5
(3) 		900.2
(-0) 		567.7
(-2) 		508
(-2.4) 		399.5
(-3) 		1012
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 618
1 2 3 4 range
A 666
(3)
P 		397.5
(0.4)
P 		409.6
(0.5)
P 		55.9
(-3)
P 		610
B 610.2
(3)
P 		467.6
(1.4)
P 		361.8
(0.2)
P 		72.2
(-3)
P 		538
C 655.7
(3)
P 		457.3
(1)
P 		469.4
(1.2)
P 		48.2
(-3)
P 		608
avr 644
(3) 		440.8
(0.9) 		413.6
(0.6) 		58.8
(-3) 		585

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 452
1 2 3 4 5 6 range
A 228.7
(-1.5)
P 		558.1
(3)
P 		154.6
(-2.5)
P 		155.6
(-2.5)
P 		124.6
(-2.9)
P 		119.9
(-3)
P 		438
B 443.4
(2.9)
P 		449.1
(3)
P 		212.4
(-1.5)
P 		149
(-2.8)
P 		176
(-2.2)
P 		136.2
(-3)
P 		313
C 551.3
(3)
P 		401.6
(1)
P 		284.1
(-0.6)
P 		106
(-3)
P 		219.4
(-1.5)
P 		114.1
(-2.9)
P 		445
avr 407.8
(1.9) 		469.6
(3) 		217
(-1.4) 		136.9
(-2.8) 		173.3
(-2.1) 		123.4
(-3) 		346
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191281_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7584
Sequence Type Exemplar sequence
Representative Public ID C44B11.3 NCBI
Target Description C44B11.3 /REP_DB=WormBase Gene ID /WP=CE24843 /GEN=mec-12 /TR=O01907 /GB=AAB54263.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=alpha tubulin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065262(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065262 NCBI Caenorhabditis elegans MEChanosensory abnormality family member (mec-12) (mec-12) mRNA, complete cds. 11/11 None
SNAP00000030518 ENSEMBL cdna:SNAP chromosome:CEL140:III:3131723:3135301:-1 11/11 None
GENEFINDER00000030524 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3131723:3139307:-1 11/11 None
C44B11.3.2 ENSEMBL cdna:known chromosome:CEL140:III:3131566:3139677:-1 gene:C44B11.3 11/11 None
C44B11.3.1 ENSEMBL cdna:known chromosome:CEL140:III:3131566:3139211:-1 gene:C44B11.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3131722-3137740(-) 99.85 99.85
Public Domain and Genome References
Gene Title alpha tubulin
Gene Symbol mec-12
Ensembl C44B11.3
Entrez Gene 175419 Entrez gene
SwissProt P91910 EMBL-EBI
WormBase CE24843 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:245270_AT tubulin alpha-6 chain (TUA6) at
HU35KSUBA:RC_AA435720_I_AT similar to alpha tubulin hs
HUGENEFL:K03460_AT similar to alpha tubulin hs
U133_X3P:G306450_3P_X_AT similar to alpha tubulin hs
MU11KSUBB:MSA.37742.0_F_AT tubulin, alpha 7 mm
MG-U74AV2:93924_F_AT tubulin, alpha 7 mm
MOE430A:1416311_S_AT tubulin, alpha 7 mm
MOUSE430A_2:1416311_S_AT tubulin, alpha 7 mm
MOUSE430_2:1416311_S_AT tubulin, alpha 7 mm
MOE430A:1448296_X_AT tubulin, alpha 7 mm
MOUSE430A_2:1448296_X_AT tubulin, alpha 7 mm
MOUSE430_2:1448296_X_AT tubulin, alpha 7 mm
MOE430A:1438611_AT Tubulin, alpha 7 (Tuba7), mRNA mm
MOUSE430A_2:1438611_AT Tubulin, alpha 7 (Tuba7), mRNA mm
MOUSE430_2:1438611_AT Tubulin, alpha 7 (Tuba7), mRNA mm
MU19KSUBA:TC16386_AT Tubulin, alpha 7 (Tuba7), mRNA mm
MU19KSUBB:TC28251_F_AT Tubulin, alpha 7 (Tuba7), mRNA mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7018 microtubule-based movement inferred from electronic annotation QuickGO AmiGO
46785 microtubule polymerization inferred from electronic annotation QuickGO AmiGO
7017 microtubule-based process inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5874 microtubule inferred from electronic annotation QuickGO AmiGO
45298 tubulin inferred from electronic annotation QuickGO AmiGO
5874 microtubule inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3924 GTPase activity inferred from electronic annotation QuickGO AmiGO
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
5200 structural constituent of cytoskeleton inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_497663 MEChanosensory abnormality family member (mec-12) [Caenorhabditis elegans] gb|AAB54263.2| Mechanosensory abnormality protein 12 [Caenorhabditis elegans] gb|AAB48241.1| alpha-tubulin MEC-12 [Caenorhabditis elegans] dbj|BAA32600.1| Alpha tubulin (tba-3) [Caenorhabditis elegans] 0.0
blast BAA22203.1 alpha-3 tubulin [Caenorhabditis elegans] 0.0
blast P50719 Tubulin alpha chain gb|AAA29167.1| alpha tubulin 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 4.3E-109
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 1.6E-77
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 5.3E-94
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 1.6E-77
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 4.3E-109
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 1.6E-77
Sequence
Target Sequence 
>C. ELEGANS:191281_S_AT
attcgacggagccctcaacgtcgacttgaccgagttccagacaaacttggttccctatcc
acgtattcacttcccgctagccacattttctccggttatctcagcggaaaaagcctatca
cgagcagctatcagtggctgaaatcactaatatgtgcttcgagccacataaccagatggt
caagtgtgatccgagacacggaaagtatatggcagtttgtctgcttttccgtggagatgt
agtgccaaaggatgtgaatgctgcaattgcaactattaagactaagagaagcattcaatt
tgtagattggtgcccaactggatttaaggtcggaatcaactaccaaccaccaacggtagt
cccaggaggtgacctggcaaaggtaccccgtgccgtgtgcatgctctccaacaccaccgc
aatcgctgaagcctgggctcgtctggaccataaattcgacctaatgtatgcgaagagagc
attcgtccactggtatgttgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTCGACGGAGCCCTCAACGTCGAC 410 7 741 Antisense
CAACGTCGACTTGACCGAGTTCCAG 289 185 756 Antisense
TCCAGACAAACTTGGTTCCCTATCC 1 399 776 Antisense
ACATTTTCTCCGGTTATCTCAGCGG 24 105 823 Antisense
AAGCCTATCACGAGCAGCTATCAGT 83 149 851 Antisense
GTGCTTCGAGCCACATAACCAGATG 525 477 894 Antisense
ATATGGCAGTTTGTCTGCTTTTCCG 57 23 947 Antisense
CAACGGTAGTCCCAGGAGGTGACCT 200 187 1091 Antisense
GAGGTGACCTGGCAAAGGTACCCCG 153 383 1106 Antisense
GGGCTCGTCTGGACCATAAATTCGA 383 493 1175 Antisense
GAGCATTCGTCCACTGGTATGTTGG 457 389 1217 Antisense
Sequence Source Affymetrix Proprietary Database