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[191271_s_at]

Description C32F10.1 /REP_DB=WormBase Gene ID /WP=CE08539 /TR=O01686 /GB=AAC24270.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=oxysterol-binding protein
Cluster 6grp_highest_10_10 > 65      6grp_high1_10_10 > 56      4grp_highest_8_8 > 38      4grp_high_8_8 > 03      6grp_highest_nonorm > 84      6grp_total > 56      4grp_total > 06      6grp_cgc > 37      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 613
1 2 3 4 range
A 705.8
(0.3)
P 		406.8
(-3)
P 		443.7
(-2.6)
P 		954.3
(3)
P 		548
B 709.2
(0.3)
P 		418.7
(-2.9)
P 		407.9
(-3)
P 		957.5
(3)
P 		550
C 771.6
(1.1)
P 		357.2
(-3)
P 		534.8
(-1.3)
P 		969.7
(3)
P 		613
avr 728.9
(0.5) 		394.2
(-3) 		462.1
(-2.3) 		960.5
(3) 		566

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 315
1 2 3 4 5 6 range
A 639.7
(0.4)
P 		702.7
(1.6)
P 		502.1
(-2.2)
P 		601
(-0.3)
P 		462
(-3)
P 		776.7
(3)
P 		315
B 550.8
(-1.9)
P 		546.6
(-2)
P 		695.8
(1.6)
P 		617.7
(-0.3)
P 		503.9
(-3)
P 		752.6
(3)
P 		249
C 614.3
(-0.3)
P 		579.1
(-1.1)
P 		639.3
(0.2)
P 		592
(-0.8)
P 		496
(-3)
P 		761.3
(3)
P 		265
avr 601.6
(-0.5) 		609.5
(-0.3) 		612.4
(-0.3) 		603.6
(-0.5) 		487.3
(-3) 		763.5
(3) 		276
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 169
1 2 3 4 range
A 257
(3)
P 		88.3
(-3)
P 		116
(-2)
P 		139.7
(-1.2)
P 		169
B 226.2
(3)
P 		103.4
(-2.6)
P 		94.5
(-3)
P 		204
(2)
P 		132
C 255.4
(3)
P 		102.7
(-3)
P 		186.4
(0.3)
P 		163.5
(-0.6)
P 		153
avr 246.2
(3) 		98.1
(-3) 		132.3
(-1.6) 		169.1
(-0.1) 		148

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 171
1 2 3 4 5 6 range
A 117.9
(-2.6)
P 		249.9
(3)
P 		109.4
(-3)
P 		152.4
(-1.2)
P 		139.4
(-1.7)
P 		189.1
(0.4)
P 		141
B 191.6
(-1.6)
P 		181.9
(-2.1)
P 		169
(-2.7)
P 		163.6
(-3)
P 		204.7
(-0.9)
P 		280.6
(3)
P 		117
C 227.7
(1.8)
P 		176.7
(-0.5)
P 		162.7
(-1.1)
P 		119
(-3)
P 		160.5
(-1.2)
P 		254.8
(3)
P 		136
avr 179.1
(-0.9) 		202.8
(0.6) 		147
(-2.9) 		145
(-3) 		168.2
(-1.6) 		241.5
(3) 		97
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191271_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2516
Sequence Type Exemplar sequence
Representative Public ID C32F10.1 NCBI
Target Description C32F10.1 /REP_DB=WormBase Gene ID /WP=CE08539 /TR=O01686 /GB=AAC24270.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=oxysterol-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059290(11), NM_182017(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059290 NCBI Caenorhabditis elegans Oxysterol Binding protein (OSBP) Related family member (obr-4) (obr-4) mRNA, complete cds. 11/11 None
NM_182017 NCBI Caenorhabditis elegans Oxysterol Binding protein (OSBP) Related family member (obr-4) (obr-4) mRNA, complete cds. 11/11 None
SNAP00000028947 ENSEMBL cdna:SNAP chromosome:CEL140:I:5841621:5842447:1 11/11 None
GENEFINDER00000028970 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5841660:5842447:1 11/11 None
C32F10.1a ENSEMBL cdna:known chromosome:CEL140:I:5837525:5842776:1 gene:C32F10.1 11/11 None
C32F10.1b ENSEMBL cdna:known chromosome:CEL140:I:5840237:5842447:1 gene:C32F10.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5837535-5842447(+) 100.0 100.0
Public Domain and Genome References
Ensembl C32F10.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC24270 Oxysterol binding protein (osbp) related protein 4, isoform a [Caenorhabditis elegans] ref|NP_491691.1| Oxysterol Binding protein (OSBP) Related family member (obr-4) [Caenorhabditis elegans] 0.0
blast CAE60450 Hypothetical protein CBG04058 [Caenorhabditis briggsae] 0.0
blast AAN84853 Oxysterol binding protein (osbp) related protein 4, isoform b [Caenorhabditis elegans] ref|NP_871817.1| Oxysterol Binding protein (OSBP) Related family member (obr-4) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000648 EMBL-EBI Oxysterol-binding protein 6.4E-42
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.1E-18
Pfam IPR000648 EMBL-EBI Oxysterol-binding protein 6.4E-42
Pfam IPR000648 EMBL-EBI Oxysterol-binding protein 8.4E-28
Pfam IPR000648 EMBL-EBI Oxysterol-binding protein 1.9E-17
Sequence
Target Sequence 
>C. ELEGANS:191271_S_AT
cttccttggtctctcaattggtgttgcaagcattggagaaggtattctaacacttcataa
ctatggtgatgagcaatacacaatcacattcccatctggatacggacgatccattatgag
tactccatggttcgaatttggtggaaaagtgaaggttgaatgtgaaaagactggatatcg
tgctgatattgagttcctcaccaaaccgttctttggtggtaaaccgcatcgaattcaagg
atcaatttgtcgtgatggtgccaagaaaccggtattgacgcttcgtggagaatggaatgg
aacaatgtatgcaaaaacacaaaatggagaagaatatgtatttgttgatgtcaaagcaaa
acctgaagttaagaaagaatgtgttcctgttatgcaacaagggaaacgggagagcagaag
attgtggagacatgtgacagcagctttgctcagaaatcgtattcaaactgccactacatc
gaaaagattcatcgagcagagacaacggaaagaagcgaaggaacgcctcgaaagtggtga
aaagtggacgccgcttcttttcgacacaactgacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTCCTTGGTCTCTCAATTGGTGTT 664 213 1416 Antisense
AGCAATACACAATCACATTCCCATC 6 77 1487 Antisense
CATTCCCATCTGGATACGGACGATC 146 217 1502 Antisense
GACGATCCATTATGAGTACTCCATG 113 379 1520 Antisense
TATTGAGTTCCTCACCAAACCGTTC 502 605 1602 Antisense
GAAACCGGTATTGACGCTTCGTGGA 645 357 1680 Antisense
GGAGACATGTGACAGCAGCTTTGCT 632 519 1841 Antisense
GCAGCTTTGCTCAGAAATCGTATTC 15 315 1855 Antisense
TATTCAAACTGCCACTACATCGAAA 523 663 1875 Antisense
TGGTGAAAAGTGGACGCCGCTTCTT 631 551 1950 Antisense
CGCTTCTTTTCGACACAACTGACAA 390 255 1967 Antisense
Sequence Source Affymetrix Proprietary Database