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[191270_at] aldo/keto reductase family

Description C56G3.2 /REP_DB=WormBase Gene ID /WP=CE04284 /TR=Q18903 /GB=AAA80407.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=aldo-keto reductase family
Cluster 6grp_total > 85      4grp_total > 10      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 115
1 2 3 4 range
A 14
(-3)
A 		57.9
(1)
A 		41.5
(-0.5)
A 		80
(3)
A 		66
B 16.2
(-2.7)
A 		15.8
(-2.7)
A 		13.1
(-3)
A 		75.4
(3)
A 		62
C 27.9
(-1.7)
A 		3.7
(-3)
A 		5.1
(-2.9)
A 		118.5
(3)
A 		115
avr 19.4
(-3) 		25.8
(-2.5) 		19.9
(-3) 		91.3
(3) 		72

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 81
1 2 3 4 5 6 range
A 9.5
(-2.9)
A 		7.8
(-3)
A 		10.5
(-2.8)
A 		88.5
(3)
A 		57
(0.7)
A 		73.1
(1.9)
A 		81
B 22
(-1)
A 		24.9
(-0.5)
A 		9.4
(-3)
A 		46.8
(3)
A 		19.6
(-1.4)
A 		30.5
(0.4)
A 		37
C 15.7
(-2.5)
A 		29.1
(-1.1)
A 		11
(-3)
A 		15.8
(-2.5)
A 		47.6
(0.8)
A 		68.9
(3)
M 		58
avr 15.7
(-2.3) 		20.6
(-1.7) 		10.3
(-3) 		50.4
(2.1) 		41.4
(1) 		57.5
(3) 		47
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 5.1
(-3)
A 		12.6
(3)
A 		10.8
(1.6)
A 		11.7
(2.3)
A 		8
B 5.2
(-2)
A 		3.9
(-2.6)
A 		3
(-3)
A 		16.1
(3)
A 		13
C 9.2
(-0.4)
A 		1.1
(-3)
A 		1.8
(-2.8)
A 		20
(3)
A 		19
avr 6.5
(-2.3) 		5.9
(-2.6) 		5.2
(-3) 		15.9
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 1.7
(-3)
A 		2.8
(-2.7)
A 		2.3
(-2.8)
A 		22.4
(3)
A 		17.2
(1.5)
A 		17.8
(1.7)
A 		21
B 7.6
(0.1)
A 		8.3
(0.6)
A 		2.3
(-3)
A 		12.4
(3)
A 		8
(0.4)
A 		11.4
(2.4)
A 		10
C 5.8
(-2.1)
A 		8.9
(-1.2)
A 		2.8
(-3)
A 		3.2
(-2.9)
A 		15.4
(0.7)
A 		23.1
(3)
M 		20
avr 5
(-2) 		6.7
(-1.3) 		2.5
(-3) 		12.7
(1.1) 		13.5
(1.4) 		17.4
(3) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191270_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16852
Sequence Type Exemplar sequence
Representative Public ID C56G3.2 NCBI
Target Description C56G3.2 /REP_DB=WormBase Gene ID /WP=CE04284 /TR=Q18903 /GB=AAA80407.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=aldo-keto reductase family
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076846(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076846 NCBI Caenorhabditis elegans C56G3.2 (C56G3.2) mRNA, complete cds. 11/11 None
SNAP00000042072 ENSEMBL cdna:SNAP chromosome:CEL140:X:7365512:7366380:1 9/11 None
C56G3.2 ENSEMBL cdna:known chromosome:CEL140:X:7365512:7366243:1 gene:C56G3.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000042074 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7365510-7366268(+) 75.04 100.0
Public Domain and Genome References
Gene Title aldo/keto reductase family
Ensembl C56G3.2
Entrez Gene 183870 Entrez gene
SwissProt Q18903 EMBL-EBI
WormBase CE35443 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA80407 Hypothetical protein C56G3.2 [Caenorhabditis elegans] ref|NP_509247.2| C56G3.2 [Caenorhabditis elegans] 5.0E-62
blast AAA80407 Hypothetical protein C56G3.2 [Caenorhabditis elegans] ref|NP_509247.2| C56G3.2 [Caenorhabditis elegans] 2.0E-61
blast AAB37976 Hypothetical protein C07D8.6 [Caenorhabditis elegans] ref|NP_509242.1| C07D8.6 [Caenorhabditis elegans] pdb|1QWK|A Chain A, Structural Genomics Of Caenorhabditis Elegans: Hypothetical 35.2 Kda Protein (Aldose Reductase Family Member) 2.0E-57
blast CAE65877 Hypothetical protein CBG11027 [Caenorhabditis briggsae] 2.0E-54
blast AAB37976 Hypothetical protein C07D8.6 [Caenorhabditis elegans] ref|NP_509242.1| C07D8.6 [Caenorhabditis elegans] pdb|1QWK|A Chain A, Structural Genomics Of Caenorhabditis Elegans: Hypothetical 35.2 Kda Protein (Aldose Reductase Family Member) 7.0E-34
blast CAE69256 Hypothetical protein CBG15305 [Caenorhabditis briggsae] 6.0E-31
Protein Domains
Database ID Description E-Value
Pfam IPR001395 EMBL-EBI Aldo/keto reductase 4.2E-24
Pfam IPR001395 EMBL-EBI Aldo/keto reductase 5.4E-25
Pfam IPR001395 EMBL-EBI Aldo/keto reductase 2.7E-15
Sequence
Target Sequence 
>C. ELEGANS:191270_AT
taatatcattcgttactcgttcacaccgcttatcctcctcttcgctcggcactctgcaac
cgccagttctctcttagactcttcttccggcttgaccccagttgcggctccccaacccgc
tacactgggcggtcaacaggcttggactaatgaacttgctcctggtagactggagggagc
acttcgtgattctctcaagaaacttcatctagaatacgttgacttgtatcttgctcatat
gccaaccgcattcagtgatgacatgagccaaaaaatcgaatcttcagtagaagacatctg
gcgtcagtttgatgcagtctacaaagccggacttgccaaggctattgggcttctgctcca
tctgagttgcgtgatgaaaatgtgctggatctcgctaaaaaaataccacaagagccccgc
tcaaattcttcttcactacgcccttgagcgtggaatcgccatcattccaaagtcagtgaa
tgaaacccgtattaaggagaacttcgaattgtttgactttgaacccactcagaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAATATCATTCGTTACTCGTTCACA 550 635 27 Antisense
CTGGGCGGTCAACAGGCTTGGACTA 65 237 151 Antisense
AATGAACTTGCTCCTGGTAGACTGG 580 157 175 Antisense
TGGAGGGAGCACTTCGTGATTCTCT 99 551 197 Antisense
TATCTTGCTCATATGCCAACCGCAT 66 659 253 Antisense
TTGCCAAGGCTATTGGGCTTCTGCT 356 701 359 Antisense
CTTCTGCTCCATCTGAGTTGCGTGA 182 221 376 Antisense
ACAAGAGCCCCGCTCAAATTCTTCT 77 113 434 Antisense
CTTCACTACGCCCTTGAGCGTGGAA 35 223 457 Antisense
AATCGCCATCATTCCAAAGTCAGTG 272 169 480 Antisense
GTTTGACTTTGAACCCACTCAGAAT 553 451 537 Antisense
Sequence Source Affymetrix Proprietary Database