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[191265_at] 7TM chemoreceptor, srh family

Description W02H5.6 /REP_DB=WormBase Gene ID /WP=CE28418 /GEN=srh-270 /TR=Q9N5D1 /GB=AAF60378.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster 6grp_total > 31      4grp_total > 23      6grp_cgc > 62      6grp_cgc_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 82.2
(3)
A 		11
(-3)
A 		16.6
(-2.5)
A 		75.3
(2.4)
A 		71
B 77.2
(3)
A 		14.7
(-3)
A 		14.9
(-3)
A 		45.9
(-0)
A 		63
C 87
(3)
A 		53.8
(-1.6)
A 		43.4
(-3)
A 		62.4
(-0.4)
A 		44
avr 82.1
(3) 		26.5
(-2.8) 		25
(-3) 		61.2
(0.8) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 83
1 2 3 4 5 6 range
A 20.9
(-2.3)
A 		81.1
(3)
A 		47.9
(0.1)
A 		61.4
(1.3)
A 		61.1
(1.3)
A 		12.3
(-3)
A 		69
B 63.9
(1.8)
A 		53.9
(0.9)
A 		66.9
(2)
A 		78.5
(3)
A 		8.4
(-3)
A 		27.9
(-1.3)
A 		70
C 56.5
(0.4)
A 		85
(2.5)
A 		91.4
(3)
A 		34.3
(-1.2)
A 		42.9
(-0.6)
A 		10.4
(-3)
A 		81
avr 47.1
(0.2) 		73.3
(3) 		68.7
(2.5) 		58.1
(1.4) 		37.5
(-0.8) 		16.9
(-3) 		56
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 28
1 2 3 4 range
A 29.9
(3)
A 		2.4
(-3)
A 		4.3
(-2.6)
A 		11
(-1.1)
A 		28
B 24.6
(3)
A 		3.6
(-3)
A 		3.5
(-3)
A 		9.8
(-1.2)
A 		21
C 28.8
(3)
A 		15.5
(-1.4)
A 		15.1
(-1.5)
A 		10.5
(-3)
A 		18
avr 27.8
(3) 		7.2
(-3) 		7.6
(-2.9) 		10.4
(-2.1) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 3.8
(-2.8)
A 		28.8
(3)
A 		10.4
(-1.3)
A 		15.6
(-0.1)
A 		18.4
(0.6)
A 		3
(-3)
A 		26
B 22.2
(3)
A 		17.9
(1.6)
A 		16.2
(1.1)
A 		20.8
(2.6)
A 		3.4
(-3)
A 		10.4
(-0.8)
A 		19
C 20.9
(1.7)
A 		25.9
(3)
A 		23.3
(2.3)
A 		6.9
(-2.1)
A 		13.9
(-0.2)
A 		3.5
(-3)
A 		22
avr 15.6
(0.2) 		24.2
(3) 		16.6
(0.5) 		14.4
(-0.2) 		11.9
(-1) 		5.6
(-3) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191265_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15822
Sequence Type Exemplar sequence
Representative Public ID W02H5.6 NCBI
Target Description W02H5.6 /REP_DB=WormBase Gene ID /WP=CE28418 /GEN=srh-270 /TR=Q9N5D1 /GB=AAF60378.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071317(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071317 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-271) (srh-271) mRNA, complete cds. 11/11 None
SNAP00000022924 ENSEMBL cdna:SNAP chromosome:CEL140:V:2618826:2620260:-1 11/11 None
W02H5.6 ENSEMBL cdna:known chromosome:CEL140:V:2618826:2620541:-1 gene:W02H5.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000022937 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2618825-2620542(-) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, srh family
Gene Symbol srh-271
Ensembl W02H5.6
Entrez Gene 189138 Entrez gene
SwissProt Q9N5D1 EMBL-EBI
WormBase CE28418 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_503718 Serpentine Receptor, class H family member (srh-271) [Caenorhabditis elegans] gb|AAF60378.2| Serpentine receptor, class h protein 271 [Caenorhabditis elegans] 1.0E-178
blast NP_503718 Serpentine Receptor, class H family member (srh-271) [Caenorhabditis elegans] gb|AAF60378.2| Serpentine receptor, class h protein 271 [Caenorhabditis elegans] 1.0E-138
blast AAK18947 Serpentine receptor, class h protein 272 [Caenorhabditis elegans] ref|NP_503776.2| Serpentine Receptor, class H family member (srh-272) [Caenorhabditis elegans] 1.0E-106
blast AAK18947 Serpentine receptor, class h protein 272 [Caenorhabditis elegans] ref|NP_503776.2| Serpentine Receptor, class H family member (srh-272) [Caenorhabditis elegans] 8.0E-76
blast AAB52307 Serpentine receptor, class h protein 275 [Caenorhabditis elegans] ref|NP_504876.1| Serpentine Receptor, class H family member (srh-275) [Caenorhabditis elegans] 1.0E-71
blast CAE58085 Hypothetical protein CBG01167 [Caenorhabditis briggsae] 6.0E-53
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.2E-119
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503718.1 7 15-34,54-76,91-113,134-153,190-212,242-264,274-293
W02H5.6 7 15-34,54-76,91-113,134-153,190-212,242-264,274-293
SNAP00000022924 5 26-48,69-88,125-147,177-199,209-228
Sequence
Target Sequence 
>C. ELEGANS:191265_AT
gtaagagtttcctatttcgctttcaactacattctggcattattattttttattcctcca
tatctgcgtataccggatcaagacctggcgttacagcaaatttacaaggagctcccatca
aatctcccaaattggattacaaaagcagacgtatttgttctagcaaccgacataacctat
tcattattatcagtgttcctcgttgtgatattcattatatcagagatcttgttcttcata
ttctacgtcaaaattcaaatgaaatgcttggctagaacgttgaaactgtccaagaccact
ttgaagatgcaacagaagtttctggatgccattcattttcagatgtacaccccattgata
attctggtaatcccattagcatacatcggatacgctatatattttgatttctacaaccaa
gctgcaaacaacatttctttcataattatcacttttcatgggcttgcctcgactattatt
atgctactgatacacaaaccgtatcgggaagtttgtgtgaacatgttctgcttcaaaatt
ctgaacaaaactactccagccggacatacga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTAAGAGTTTCCTATTTCGCTTTCA 563 461 487 Antisense
TCGCTTTCAACTACATTCTGGCATT 211 601 503 Antisense
TCCTCCATATCTGCGTATACCGGAT 461 593 540 Antisense
ATACCGGATCAAGACCTGGCGTTAC 391 1 556 Antisense
ACAAGGAGCTCCCATCAAATCTCCC 519 113 590 Antisense
GACGTATTTGTTCTAGCAACCGACA 680 377 634 Antisense
CAGTGTTCCTCGTTGTGATATTCAT 172 209 677 Antisense
GATCTTGTTCTTCATATTCTACGTC 255 421 711 Antisense
CATTTTCAGATGTACACCCCATTGA 516 215 820 Antisense
GGGCTTGCCTCGACTATTATTATGC 581 493 946 Antisense
AAAACTACTCCAGCCGGACATACGA 313 129 1033 Antisense
Sequence Source Affymetrix Proprietary Database