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[191263_at] protein kinase C substrate

Description ZK1307.8 /REP_DB=WormBase Gene ID /WP=CE15547 /TR=Q23440 /GB=CAA87438.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=protein kinase C substrate
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 63      4grp_highest_8_8 > 08      4grp_high_8_8 > 52      6grp_highest_nonorm > 66      6grp_total > 63      4grp_total > 59      6grp_highest_sub > 03_04      6grp_high1_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 641
1 2 3 4 range
A 899.1
(-1.2)
P 		1342.6
(3)
P 		959.7
(-0.6)
P 		701.6
(-3)
P 		641
B 1136
(1.1)
P 		1230.8
(2)
P 		1330.1
(3)
P 		704.7
(-3)
P 		625
C 1025.9
(1.8)
P 		1036.7
(2)
P 		1100
(3)
P 		732.4
(-3)
P 		368
avr 1020.3
(0.8) 		1203.4
(3) 		1129.9
(2.1) 		712.9
(-3) 		491

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 991
1 2 3 4 5 6 range
A 919.8
(-3)
P 		1579.2
(1.4)
P 		1701.5
(2.2)
P 		1823.9
(3)
P 		1173.8
(-1.3)
P 		1349.2
(-0.2)
P 		904
B 1003.3
(-3)
P 		1227.6
(-1.3)
P 		1817.2
(3)
P 		1475.1
(0.5)
P 		1237.2
(-1.3)
P 		1411.7
(0)
P 		814
C 1251.1
(-3)
P 		1334.4
(-2.2)
P 		1910.5
(3)
P 		1705.6
(1.1)
P 		1263.2
(-2.9)
P 		1456.5
(-1.1)
P 		659
avr 1058.1
(-3) 		1380.4
(-0.4) 		1809.7
(3) 		1668.2
(1.9) 		1224.7
(-1.7) 		1405.8
(-0.2) 		752
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 281
1 2 3 4 range
A 327.4
(3)
P 		291.4
(2)
P 		250.9
(1)
P 		102.7
(-3)
P 		225
B 362.3
(3)
P 		304
(1.4)
P 		308.2
(1.5)
P 		150.2
(-3)
P 		212
C 339.6
(2)
P 		298.1
(1)
P 		383.4
(3)
P 		123.5
(-3)
P 		260
avr 343.1
(3) 		297.8
(1.8) 		314.2
(2.2) 		125.5
(-3) 		218

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 392
1 2 3 4 5 6 range
A 169.6
(-3)
P 		561.7
(3)
P 		370.6
(0.1)
P 		462.6
(1.5)
P 		354.2
(-0.2)
P 		328.5
(-0.6)
P 		392
B 349.1
(-3)
P 		408.6
(-1)
P 		441.3
(0.1)
P 		390.7
(-1.6)
P 		502.7
(2.2)
P 		526.4
(3)
P 		177
C 463.7
(2)
P 		407.2
(-0.3)
P 		486.3
(2.9)
P 		343
(-3)
P 		408.8
(-0.3)
P 		487.6
(3)
P 		145
avr 327.5
(-3) 		459.2
(3) 		432.7
(1.8) 		398.8
(0.2) 		421.9
(1.3) 		447.5
(2.5) 		132
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191263_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3486
Sequence Type Exemplar sequence
Representative Public ID ZK1307.8 NCBI
Target Description ZK1307.8 /REP_DB=WormBase Gene ID /WP=CE15547 /TR=Q23440 /GB=CAA87438.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=protein kinase C substrate
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063672(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063672 NCBI Caenorhabditis elegans ZK1307.8 (ZK1307.8) mRNA, complete cds. 11/11 None
SNAP00000021420 ENSEMBL cdna:SNAP chromosome:CEL140:II:9625078:9626325:1 11/11 None
GENEFINDER00000021433 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9625192:9626325:1 11/11 None
ZK1307.8.1 ENSEMBL cdna:known chromosome:CEL140:II:9624524:9626535:1 gene:ZK1307.8 11/11 None
ZK1307.8.2 ENSEMBL cdna:known chromosome:CEL140:II:9624524:9626861:1 gene:ZK1307.8 11/11 None
ZK1307.8.3 ENSEMBL cdna:known chromosome:CEL140:II:9624548:9626527:1 gene:ZK1307.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9624546-9626322(+) 100.0 100.0
Public Domain and Genome References
Gene Title protein kinase C substrate
Ensembl ZK1307.8
Entrez Gene 174515 Entrez gene
SwissProt Q23440 EMBL-EBI
WormBase CE15547 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:247990_AT calmodulin-binding protein at
CANINE_2:CFA.17034.1.S1_AT similar to Glucosidase II beta subunit precursor (Protein kinase C substrate, 60.1 kDa protein, heavy chain) (PKCSH) (80K-H protein) cfa
CANINE_2:CFA.21566.1.S1_AT similar to Glucosidase II beta subunit precursor (Protein kinase C substrate, 60.1 kDa protein, heavy chain) (PKCSH) (80K-H protein) cfa
CANINE_2:CFA.21566.1.S1_S_AT similar to Glucosidase II beta subunit precursor (Protein kinase C substrate, 60.1 kDa protein, heavy chain) (PKCSH) (80K-H protein) cfa
CANINE_2:CFAAFFX.26607.1.S1_S_AT similar to Glucosidase II beta subunit precursor (Protein kinase C substrate, 60.1 kDa protein, heavy chain) (PKCSH) (80K-H protein) cfa
DROSOPHILA_2:1625519_AT dm
DROSGENOME1:141204_AT dm
HG-U133_PLUS_2:200707_AT protein kinase C substrate 80K-H hs
HG-U133A:200707_AT protein kinase C substrate 80K-H hs
HG-FOCUS:200707_AT protein kinase C substrate 80K-H hs
HG-U133A_2:200707_AT protein kinase C substrate 80K-H hs
HG-U133_PLUS_2:214080_X_AT protein kinase C substrate 80K-H hs
HG-U133A:214080_X_AT protein kinase C substrate 80K-H hs
HG-U133A_2:214080_X_AT protein kinase C substrate 80K-H hs
HUGENEFL:U50327_S_AT protein kinase C substrate 80K-H hs
U133_X3P:G4506076_3P_X_AT protein kinase C substrate 80K-H hs
U133_X3P:G4506076_3P_A_AT protein kinase C substrate 80K-H hs
U133_X3P:HS.1432.1.S1_3P_A_AT protein kinase C substrate 80K-H hs
U133_X3P:214080_3P_X_AT protein kinase C substrate 80K-H hs
U133_X3P:200707_3P_AT protein kinase C substrate 80K-H hs
HG-U95AV2:39711_AT protein kinase C substrate 80K-H hs
HG-U95C:63705_F_AT protein kinase C substrate 80K-H hs
MU11KSUBA:U92794_S_AT protein kinase C substrate 80K-H mm
MU11KSUBB:MSA.9765.0_S_AT protein kinase C substrate 80K-H mm
MU11KSUBB:MSA.18394.0_S_AT protein kinase C substrate 80K-H mm
MU11KSUBB:MSA.6022.0_S_AT protein kinase C substrate 80K-H mm
MU11KSUBB:MSA.23422.0_S_AT protein kinase C substrate 80K-H mm
MG-U74AV2:100684_AT protein kinase C substrate 80K-H mm
MOE430A:1416339_A_AT protein kinase C substrate 80K-H mm
MOUSE430A_2:1416339_A_AT protein kinase C substrate 80K-H mm
MOUSE430_2:1416339_A_AT protein kinase C substrate 80K-H mm
RAE230A:1388371_AT Protein kinase C substrate 80K-H (predicted) rn
RAT230_2:1388371_AT Protein kinase C substrate 80K-H (predicted) rn
RG-U34B:RC_AI008784_AT Protein kinase C substrate 80K-H (predicted) rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA87438 Hypothetical protein ZK1307.8 [Caenorhabditis elegans] emb|CAA87420.1| Hypothetical protein ZK1307.8 [Caenorhabditis elegans] ref|NP_496073.1| ZK1307.8 [Caenorhabditis elegans] 0.0
blast CAE57913 Hypothetical protein CBG00964 [Caenorhabditis briggsae] 0.0
blast CAE57913 Hypothetical protein CBG00964 [Caenorhabditis briggsae] 1.0E-160
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 2.6E-5
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 2.6E-5
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 3.4E-5
Sequence
Target Sequence 
>C. ELEGANS:191263_AT
ggatattgcccacccagatgaggatgacgagaagatgccagactatccaccagaagttca
acaattaactgaaaaagcacgtgaagcacgtcgactattcgatgaagttaatacgaaagt
tcaagaccttgacgcaaagattcgtgaagctgaagactttgctaactcagattatggcga
ggacagcgcgtgggctgcacttaaagacaaatgttttgatagaaatgtccaacagtacac
atatcaattctgtccatttggacaaaatactcaaaaagatacgggcgcctacagtggaac
ttcacttggaagtttcaaggaatggagtggcccagaaggaaataagtactccaagatgca
ttttggagatggacaacagtgttggaatggaccaaagaggagcactgatatcaccattga
atgcggagaagaaaatgagcttgttgaagtcacggagccagcaaaatgcgagtatctctt
caccttccgaactccattggcatgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATATTGCCCACCCAGATGAGGAT 581 509 993 Antisense
ATGCCAGACTATCCACCAGAAGTTC 212 41 1027 Antisense
ACGTGAAGCACGTCGACTATTCGAT 68 95 1071 Antisense
GACTTTGCTAACTCAGATTATGGCG 82 371 1147 Antisense
TTATGGCGAGGACAGCGCGTGGGCT 251 679 1164 Antisense
AGCGCGTGGGCTGCACTTAAAGACA 368 81 1177 Antisense
AGATACGGGCGCCTACAGTGGAACT 272 65 1269 Antisense
GAGGAGCACTGATATCACCATTGAA 182 405 1389 Antisense
AGCTTGTTGAAGTCACGGAGCCAGC 678 63 1430 Antisense
AAAATGCGAGTATCTCTTCACCTTC 269 133 1455 Antisense
ACCTTCCGAACTCCATTGGCATGTG 320 87 1474 Antisense
Sequence Source Affymetrix Proprietary Database