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[191261_at] G-protein coupled receptor

Description T23B3.4 /REP_DB=WormBase Gene ID /WP=CE14014 /TR=P91487 /GB=AAB42331.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster 6grp_high1_10_10 > 15      6grp_total > 25      4grp_total > 13      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 252
1 2 3 4 range
A 228
(2.7)
P 		106.7
(-3)
M 		118.7
(-2.4)
A 		235.5
(3)
P 		129
B 195.1
(-2.1)
P 		171
(-3)
A 		188.7
(-2.3)
P 		331.1
(3)
P 		160
C 215.5
(-0.5)
P 		147.5
(-2.1)
A 		110.1
(-3)
A 		358.6
(3)
P 		249
avr 212.9
(-0.4) 		141.7
(-2.9) 		139.2
(-3) 		308.4
(3) 		169

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 131
1 2 3 4 5 6 range
A 208.4
(2.8)
P 		212.4
(3)
P 		118
(-1.3)
A 		139.5
(-0.3)
P 		81
(-3)
A 		130.2
(-0.8)
A 		131
B 157.3
(2.6)
M 		161.5
(3)
P 		91.1
(-3)
P 		108.8
(-1.5)
A 		130.3
(0.3)
P 		147.5
(1.8)
A 		70
C 174.7
(0.9)
P 		211.5
(3)
P 		178.8
(1.1)
P 		180.7
(1.2)
A 		106.6
(-3)
A 		117.9
(-2.4)
P 		105
avr 180.1
(2) 		195.1
(3) 		129.3
(-1.4) 		143
(-0.5) 		106
(-3) 		131.9
(-1.3) 		89
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 83
(3)
P 		23.2
(-3)
M 		31
(-2.2)
A 		34.5
(-1.9)
P 		60
B 62.2
(1.2)
P 		42.2
(-3)
A 		43.7
(-2.7)
P 		70.6
(3)
P 		28
C 71.3
(3)
P 		42.4
(-2.3)
A 		38.4
(-3)
A 		60.5
(1)
P 		33
avr 72.2
(3) 		35.9
(-3) 		37.7
(-2.7) 		55.2
(0.2) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 38.4
(-1.4)
P 		75.5
(3)
P 		25.7
(-2.8)
A 		35.4
(-1.7)
P 		24.4
(-3)
A 		31.7
(-2.1)
A 		51
B 54.7
(2.9)
M 		53.7
(2.8)
P 		22.1
(-3)
P 		28.8
(-1.8)
A 		52.9
(2.6)
P 		55
(3)
A 		33
C 64.8
(3)
P 		64.5
(2.9)
P 		45.5
(-0.8)
P 		36.3
(-2.6)
A 		34.5
(-3)
A 		39.5
(-2)
P 		30
avr 52.6
(0.9) 		64.6
(3) 		31.1
(-3) 		33.5
(-2.6) 		37.3
(-1.9) 		42.1
(-1) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191261_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2317
Sequence Type Exemplar sequence
Representative Public ID T23B3.4 NCBI
Target Description T23B3.4 /REP_DB=WormBase Gene ID /WP=CE14014 /TR=P91487 /GB=AAB42331.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059517(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059517 NCBI Caenorhabditis elegans T23B3.4 (T23B3.4) mRNA, complete cds. 11/11 None
SNAP00000020980 ENSEMBL cdna:SNAP chromosome:CEL140:I:6695630:6698412:-1 11/11 None
GENEFINDER00000020992 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6695630:6699796:-1 11/11 None
T23B3.4 ENSEMBL cdna:known chromosome:CEL140:I:6695630:6698412:-1 gene:T23B3.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6695634-6698341(-) 100.0 100.0
Public Domain and Genome References
Gene Title G-protein coupled receptor
Ensembl T23B3.4
Entrez Gene 188774 Entrez gene
SwissProt P91487 EMBL-EBI
WormBase CE31369 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
1584 rhodopsin-like receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB42331 Hypothetical protein T23B3.4 [Caenorhabditis elegans] ref|NP_491918.2| T23B3.4 [Caenorhabditis elegans] 0.0
blast CAE67259 Hypothetical protein CBG12702 [Caenorhabditis briggsae] 0.0
blast CAE67258 Hypothetical protein CBG12701 [Caenorhabditis briggsae] 3.0E-82
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 9.3E-59
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 4.2E-55
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_491918.2 6 25-47,62-84,105-127,147-169,282-304,319-341
T23B3.4 6 25-47,62-84,105-127,147-169,282-304,319-341
SNAP00000020980 6 25-47,62-84,105-127,147-169,282-304,319-341
GENEFINDER00000020992 7 15-39,51-73,100-122,143-165,185-207,320-342,357-379
Sequence
Target Sequence 
>C. ELEGANS:191261_AT
tgattgaaaaaactctcccgtctcgtgcttcattttcggattggtttgttagtgcagtgc
aacgagttccaagtatgaaagttgtttcaaaaacttttcaattcaaagagaaaaactctc
tgtcaattcctcaaacaagcggattgtctgttcgtcccagtagatcatcgttttcatcct
ttttctcaacaccacgtggatcgtttgatgttacgatgcttttaaggagcacaaatcaag
aaaagatcctgatcgccaagaagaaagtgacccgaatgttgataacacttgtgattgttt
ttgcattttgttgggtgccatcttacctgtattggctactactcagaatggctgaactag
cggcgactgatttgtggaatcctggactcaactcatcattgacaatcatgacatacatca
gctctttggcaaatccaatcacctactgtttcatgaataaatcatttcgctcctcggtat
tagcttactgtcgtccaaaaccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGATTGAAAAAACTCTCCCGTCTCG 677 563 617 Antisense
CCGTCTCGTGCTTCATTTTCGGATT 335 265 634 Antisense
AAAACTCTCTGTCAATTCCTCAAAC 308 131 728 Antisense
ATTGTCTGTTCGTCCCAGTAGATCA 103 3 759 Antisense
AGATCATCGTTTTCATCCTTTTTCT 99 67 778 Antisense
TCCTTTTTCTCAACACCACGTGGAT 489 591 793 Antisense
GGGTGCCATCTTACCTGTATTGGCT 356 497 929 Antisense
GTGGAATCCTGGACTCAACTCATCA 556 491 990 Antisense
GCAAATCCAATCACCTACTGTTTCA 92 317 1045 Antisense
TTTCGCTCCTCGGTATTAGCTTACT 286 671 1081 Antisense
TTAGCTTACTGTCGTCCAAAACCAA 364 681 1096 Antisense
Sequence Source Affymetrix Proprietary Database