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[191253_at] di-hydrofolate reductase

Description C36B1.7 /REP_DB=WormBase Gene ID /WP=CE05374 /TR=SW:Q93341 /GB=CAB02272.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=di-hydrofolate reductase
Cluster 6grp_highest_10_10 > 58      6grp_high1_10_10 > 85      4grp_highest_8_8 > 05      4grp_high_8_8 > 29      6grp_highest_nonorm > 74      6grp_total > 75      4grp_total > 26      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 252
1 2 3 4 range
A 428.1
(-3)
P 		597.3
(1.4)
P 		449.7
(-2.4)
P 		656.6
(3)
P 		229
B 411.3
(-3)
P 		558
(0.5)
P 		556.2
(0.4)
P 		663.7
(3)
P 		252
C 488.9
(-2.8)
P 		657.1
(3)
P 		482
(-3)
P 		605.1
(1.2)
P 		175
avr 442.8
(-3) 		604.1
(1.9) 		496
(-1.4) 		641.8
(3) 		199

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 529
1 2 3 4 5 6 range
A 537.4
(-2.6)
P 		514.3
(-3)
P 		885.3
(3)
P 		699.7
(-0)
P 		726.7
(0.4)
P 		768.4
(1.1)
P 		371
B 498.8
(-3)
P 		674.3
(-1)
P 		934.7
(1.9)
P 		798.7
(0.4)
P 		771.5
(0.1)
P 		1027.5
(3)
P 		529
C 680.2
(-1.6)
P 		667.1
(-1.9)
P 		617.7
(-3)
P 		768.9
(0.3)
P 		775.7
(0.5)
P 		889.3
(3)
P 		272
avr 572.1
(-3) 		618.6
(-2.1) 		812.6
(1.5) 		755.8
(0.4) 		758
(0.5) 		895.1
(3) 		323
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 155.9
(3)
P 		129.7
(0.4)
P 		117.5
(-0.9)
P 		96.1
(-3)
P 		60
B 131.2
(-1.9)
P 		137.8
(1.3)
P 		128.9
(-3)
P 		141.4
(3)
P 		13
C 161.9
(1.1)
P 		188.9
(3)
P 		168
(1.6)
P 		102
(-3)
P 		87
avr 149.7
(2.6) 		152.1
(3) 		138.1
(0.8) 		113.2
(-3) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 284
1 2 3 4 5 6 range
A 99.1
(-3)
P 		182.9
(1.2)
P 		192.8
(1.7)
P 		177.5
(0.9)
P 		219.3
(3)
P 		187.1
(1.4)
P 		120
B 173.6
(-3)
P 		224.4
(-1.5)
P 		227
(-1.5)
P 		211.5
(-1.9)
P 		313.4
(1)
P 		383.1
(3)
P 		210
C 252.1
(1.1)
P 		203.6
(-0.9)
P 		157.2
(-2.9)
P 		154.6
(-3)
P 		251
(1)
P 		297.7
(3)
P 		143
avr 174.9
(-3) 		203.6
(-1.5) 		192.3
(-2.1) 		181.2
(-2.7) 		261.2
(1.5) 		289.3
(3) 		114
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191253_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2038
Sequence Type Exemplar sequence
Representative Public ID C36B1.7 NCBI
Target Description C36B1.7 /REP_DB=WormBase Gene ID /WP=CE05374 /TR=SW:Q93341 /GB=CAB02272.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=di-hydrofolate reductase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059963(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059963 NCBI Caenorhabditis elegans C36B1.7 (C36B1.7) mRNA, complete cds. 11/11 None
GENEFINDER00000022511 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8744347:8745048:1 11/11 None
SNAP00000022504 ENSEMBL cdna:SNAP chromosome:CEL140:I:8744356:8749642:1 11/11 None
C36B1.7 ENSEMBL cdna:known chromosome:CEL140:I:8744344:8745048:1 gene:C36B1.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8744277-8744979(+) 100.0 100.0
Public Domain and Genome References
Gene Title di-hydrofolate reductase
Ensembl C36B1.7
Entrez Gene 172681 Entrez gene
SwissProt Q93341 EMBL-EBI
WormBase CE05374 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1590758_AT similar to Dihydrofolate reductase cfa
CANINE_2:CFA.5529.1.S1_AT similar to Dihydrofolate reductase cfa
CANINE_2:CFAAFFX.6880.1.S1_S_AT similar to Dihydrofolate reductase cfa
DROSOPHILA_2:1639972_AT Dihydrofolate reductase dm
DROSGENOME1:143423_AT Dihydrofolate reductase dm
CHICKEN:GGA.2883.1.S1_AT dihydrofolate reductase gga
CHICKEN:GGAAFFX.11934.1.S1_S_AT dihydrofolate reductase gga
HG-U133A:202534_X_AT dihydrofolate reductase hs
HG-U133_PLUS_2:202534_X_AT dihydrofolate reductase hs
HG-U133A_2:202534_X_AT dihydrofolate reductase hs
HG-U133_PLUS_2:202532_S_AT dihydrofolate reductase hs
HG-U133A:202532_S_AT dihydrofolate reductase hs
HG-FOCUS:202532_S_AT dihydrofolate reductase hs
HG-U133A_2:202532_S_AT dihydrofolate reductase hs
HU35KSUBB:RC_N52980_S_AT dihydrofolate reductase hs
HU35KSUBB:RC_AA129465_F_AT dihydrofolate reductase hs
HU35KSUBD:RC_AA423962_S_AT dihydrofolate reductase hs
HG-U95AV2:1610_S_AT dihydrofolate reductase hs
HC-G110:1610_S_AT dihydrofolate reductase hs
HUGENEFL:J00139_S_AT dihydrofolate reductase hs
HG-U133A:202533_S_AT dihydrofolate reductase hs
HG-U133_PLUS_2:202533_S_AT dihydrofolate reductase hs
HG-U133A_2:202533_S_AT dihydrofolate reductase hs
U133_X3P:G8924154_3P_S_AT dihydrofolate reductase hs
U133_X3P:G7262376_3P_S_AT dihydrofolate reductase hs
U133_X3P:G13097773_3P_A_AT dihydrofolate reductase hs
U133_X3P:G12652874_3P_X_AT dihydrofolate reductase hs
U133_X3P:G12652874_3P_S_AT dihydrofolate reductase hs
U133_X3P:G12652874_3P_A_AT dihydrofolate reductase hs
U133_X3P:48808_3P_AT dihydrofolate reductase hs
U133_X3P:4878663C_3P_S_AT dihydrofolate reductase hs
U133_X3P:202534_3P_X_AT dihydrofolate reductase hs
U133_X3P:202532_3P_S_AT dihydrofolate reductase hs
HG-U95AV2:37913_AT dihydrofolate reductase hs
HG-U133A_2:48808_AT dihydrofolate reductase hs
HG-U133_PLUS_2:48808_AT dihydrofolate reductase hs
HG-U133A:48808_AT dihydrofolate reductase hs
HG-U95C:48808_AT dihydrofolate reductase hs
HG-U95C:64237_R_AT dihydrofolate reductase hs
HG-U133_PLUS_2:233263_AT Dihydrofolate reductase hs
HG-U133B:233263_AT Dihydrofolate reductase hs
U133_X3P:HS.296708.0.S1_3P_AT Dihydrofolate reductase hs
MU11KSUBA:L26316_S_AT dihydrofolate reductase mm
MG-U74AV2:104547_AT dihydrofolate reductase mm
MG-U74BV2:105728_AT dihydrofolate reductase mm
MG-U74BV2:105527_AT dihydrofolate reductase mm
MOE430A:1419172_AT dihydrofolate reductase mm
MOUSE430A_2:1419172_AT dihydrofolate reductase mm
MOUSE430_2:1419172_AT dihydrofolate reductase mm
MOE430B:1430750_AT dihydrofolate reductase mm
MOUSE430_2:1430750_AT dihydrofolate reductase mm
RT-U34:RC_AA900413_AT dihydrofolate reductase rn
RG-U34A:RC_AA900413_AT dihydrofolate reductase rn
RAE230A:1370075_AT dihydrofolate reductase rn
RAT230_2:1370075_AT dihydrofolate reductase rn
YEAST_2:1779549_AT Dihydrofolate reductase, part of the dTTP biosynthetic pathway, involved in folate metabolism, possibly required for mitochondrial function Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6545 glycine biosynthesis inferred from electronic annotation QuickGO AmiGO
9165 nucleotide biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4146 dihydrofolate reductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02273 Hypothetical protein C36B1.8a [Caenorhabditis elegans] ref|NP_492365.3| C36B1.8a [Caenorhabditis elegans] 0.0
blast CAD56562 Hypothetical protein C36B1.8b [Caenorhabditis elegans] ref|NP_871882.2| C36B1.8b [Caenorhabditis elegans] 0.0
blast CAE66963 Hypothetical protein CBG12357 [Caenorhabditis briggsae] 1.0E-175
blast CAB02272 Hypothetical protein C36B1.7 [Caenorhabditis elegans] ref|NP_492364.1| C36B1.7 [Caenorhabditis elegans] sp|Q93341|DYR_CAEEL Putative dihydrofolate reductase 1.0E-104
blast CAE66962 Hypothetical protein CBG12356 [Caenorhabditis briggsae] 4.0E-85
blast EAL28532 GA13324-PA [Drosophila pseudoobscura] 5.0E-34
Protein Domains
Database ID Description E-Value
Pfam IPR001796 EMBL-EBI Dihydrofolate reductase 4.7E-72
Pfam IPR001796 EMBL-EBI Dihydrofolate reductase 8.1E-70
Sequence
Target Sequence 
>C. ELEGANS:191253_AT
tggcgtgttgccatggcgaatcaagaaagacatgcagtatttcgcgtctgttaccaaaaa
tgtttctgatcagtcgaagagaaatgcagttctcatgggacgaaaatgctgggaaagcat
tccagtaactcgccgcccgttggcaggacggctaaacattgttttgagccgacaacttcc
tgcgcaaaagtccgatgactacattgttgtaaactcgctcgaagcggctatgaaactact
ttcggagccaccatttgtggattcgatcgaaacaatctggaatatcggtggagctgaaat
ctatgatcttgcgttgcgagaaaacttggttgatgaaattcacttgacacgtattttcaa
aaacttcgaagccgatgttcatctcaagtcactcgatttca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGCGTGTTGCCATGGCGAATCAAG 246 545 69 Antisense
CAGTATTTCGCGTCTGTTACCAAAA 466 205 103 Antisense
GGGAAAGCATTCCAGTAACTCGCCG 34 495 179 Antisense
CGCCCGTTGGCAGGACGGCTAAACA 159 257 202 Antisense
ATTGTTTTGAGCCGACAACTTCCTG 490 3 226 Antisense
CAACTTCCTGCGCAAAAGTCCGATG 364 187 241 Antisense
GTAAACTCGCTCGAAGCGGCTATGA 193 461 277 Antisense
CTACTTTCGGAGCCACCATTTGTGG 370 225 304 Antisense
GAAATCTATGATCTTGCGTTGCGAG 347 363 364 Antisense
AACTTCGAAGCCGATGTTCATCTCA 380 139 430 Antisense
GTTCATCTCAAGTCACTCGATTTCA 195 445 445 Antisense
Sequence Source Affymetrix Proprietary Database