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[191250_at] G-protein coupled receptor

Description F35G8.1 /REP_DB=WormBase Gene ID /WP=CE07176 /TR=Q20067 /GB=AAA98009.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster 6grp_high1_10_10 > 15      4grp_high_8_8 > 10      6grp_total > 05      4grp_total > 22      6grp_high1_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 206
1 2 3 4 range
A 240
(1.1)
P 		142.9
(-3)
P 		156.7
(-2.4)
P 		283.3
(3)
P 		140
B 301.8
(3)
P 		189.5
(-0.3)
P 		95.8
(-3)
P 		271.6
(2.1)
P 		206
C 264.9
(2.8)
P 		196.8
(-1.9)
P 		181.3
(-3)
M 		267.9
(3)
P 		87
avr 268.9
(2.8) 		176.4
(-1.5) 		144.6
(-3) 		274.3
(3) 		130

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 227
1 2 3 4 5 6 range
A 301.8
(3)
P 		247.7
(1.6)
P 		145.9
(-1.1)
P 		137.1
(-1.4)
P 		232.6
(1.2)
P 		74.8
(-3)
A 		227
B 281.8
(3)
P 		261
(2.2)
P 		143.4
(-2.1)
P 		189.8
(-0.4)
P 		176.5
(-0.9)
P 		118.7
(-3)
P 		163
C 237.2
(1.8)
P 		266.8
(3)
P 		185.4
(-0.4)
P 		169.2
(-1.1)
P 		177.5
(-0.8)
P 		124.7
(-3)
P 		142
avr 273.6
(3) 		258.5
(2.5) 		158.2
(-1.1) 		165.4
(-0.9) 		195.5
(0.2) 		106.1
(-3) 		168
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 87.4
(3)
P 		31
(-3)
P 		41
(-1.9)
P 		41.5
(-1.9)
P 		56
B 96.2
(3)
P 		46.8
(-1)
P 		22.2
(-3)
P 		57.9
(-0.1)
P 		74
C 87.7
(3)
P 		56.6
(-1.4)
P 		63.2
(-0.5)
M 		45.2
(-3)
P 		43
avr 90.4
(3) 		44.8
(-2.7) 		42.1
(-3) 		48.2
(-2.2) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 80
1 2 3 4 5 6 range
A 55.6
(0.2)
P 		88.1
(3)
P 		31.8
(-1.8)
P 		34.8
(-1.6)
P 		70.2
(1.5)
P 		18.2
(-3)
A 		70
B 98
(3)
P 		86.9
(1.9)
P 		34.8
(-3)
P 		50.3
(-1.5)
P 		71.7
(0.5)
P 		44.3
(-2.1)
P 		63
C 87.9
(3)
P 		81.4
(2.3)
P 		47.2
(-1.5)
P 		34
(-3)
P 		57.4
(-0.4)
P 		41.8
(-2.1)
P 		54
avr 80.5
(2.4) 		85.5
(3) 		37.9
(-2.6) 		39.7
(-2.4) 		66.4
(0.7) 		34.8
(-3) 		51
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191250_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16552
Sequence Type Exemplar sequence
Representative Public ID F35G8.1 NCBI
Target Description F35G8.1 /REP_DB=WormBase Gene ID /WP=CE07176 /TR=Q20067 /GB=AAA98009.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077169(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077169 NCBI Caenorhabditis elegans F35G8.1 (F35G8.1) mRNA, complete cds. 11/11 None
GENEFINDER00000038534 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9315594:9319110:-1 11/11 None
F35G8.1 ENSEMBL cdna:known chromosome:CEL140:X:9315366:9321089:-1 gene:F35G8.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000038532 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9315593-9321089(-) 100.0 100.0
Public Domain and Genome References
Gene Title G-protein coupled receptor
Ensembl F35G8.1
Entrez Gene 185321 Entrez gene
SwissProt Q20067 EMBL-EBI
WormBase CE37231 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
1584 rhodopsin-like receptor activity inferred from electronic annotation QuickGO AmiGO
4983 neuropeptide Y receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA98009 Hypothetical protein F35G8.1 [Caenorhabditis elegans] ref|NP_509570.2| F35G8.1 [Caenorhabditis elegans] 0.0
blast CAE69836 Hypothetical protein CBG16160 [Caenorhabditis briggsae] 0.0
blast AAK58856 putative neuropeptide receptor NPR1 [Girardia tigrina] 1.0E-36
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.3E-34
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.3E-34
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509570.2 7 50-72,84-106,121-143,162-184,215-237,268-290,310-332
F35G8.1 7 50-72,84-106,121-143,162-184,215-237,268-290,310-332
GENEFINDER00000038534 7 27-49,61-83,98-120,139-161,192-214,245-267,287-309
Sequence
Target Sequence 
>C. ELEGANS:191250_AT
ggaccatgccatttttgcctggaatcctgggaccacgacaaacaacactaccgacttttc
tacggactctcggtgctcgtgatccgctccgcaattccacttgttctcatttccctgtgc
cactggagaatcgcagttattttgaacacgcagacgaagaaattccaaacattacgtagt
gccagcacagtcacccaatcgactgacatccgccgtaaacaacgtcttcagactctctta
ttggcaatggtggtcatatttgccgtgtccagtctcccactggacctttcaaatgttctt
caagatttgatcgtagtgtaccaggttcgacctgtccccgacaacgttcgccattttatc
ttcttcttttgccattggaccgcaatggcaggaacacttctcaacccgttagtctacgcg
tactacaacgaaaacttccggcgtcagattcaaacatgctttggagaaatgcgtggacag
ggagagttcaagcgggggctatactctattgtctccggcagatactcctaccgagcagac
gac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACCATGCCATTTTTGCCTGGAAT 524 523 583 Antisense
CACTACCGACTTTTCTACGGACTCT 599 193 628 Antisense
TCATTTCCCTGTGCCACTGGAGAAT 373 615 689 Antisense
GACATCCGCCGTAAACAACGTCTTC 674 369 787 Antisense
TGGTGGTCATATTTGCCGTGTCCAG 143 553 830 Antisense
CGACAACGTTCGCCATTTTATCTTC 253 253 921 Antisense
TTATCTTCTTCTTTTGCCATTGGAC 217 681 938 Antisense
GACCGCAATGGCAGGAACACTTCTC 138 383 960 Antisense
TTCTCAACCCGTTAGTCTACGCGTA 370 681 980 Antisense
TATACTCTATTGTCTCCGGCAGATA 616 659 1082 Antisense
CAGATACTCCTACCGAGCAGACGAC 231 207 1101 Antisense
Sequence Source Affymetrix Proprietary Database