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[191240_at] MAP kinase phosphatase like

Description F28C6.8 /REP_DB=WormBase Gene ID /WP=CE09746 /TR=Q93622 /GB=CAA92677.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=MAP kinase phosphatase like
Cluster 6grp_highest_10_10 > 46      6grp_high1_10_10 > 64      4grp_highest_8_8 > 08      4grp_high_8_8 > 44      6grp_highest_nonorm > 42      6grp_total > 54      4grp_total > 51      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 660
1 2 3 4 range
A 802.9
(0.6)
P 		1061.8
(3)
P 		741.3
(-0)
P 		424.4
(-3)
P 		637
B 508.1
(-2.6)
P 		1083.9
(3)
P 		807.8
(0.3)
P 		461.5
(-3)
P 		622
C 867.9
(1.3)
P 		839
(1)
P 		1017.6
(3)
P 		488.3
(-3)
P 		529
avr 726.3
(-0) 		994.9
(3) 		855.6
(1.4) 		458.1
(-3) 		537

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 830
1 2 3 4 5 6 range
A 771.7
(-1.6)
P 		911
(-0.5)
P 		1343.9
(3)
P 		909.8
(-0.5)
P 		599.4
(-3)
P 		860.6
(-0.9)
P 		745
B 768.8
(-1.8)
P 		939.5
(-0.5)
P 		1429.2
(3)
P 		875.1
(-1)
P 		598.8
(-3)
P 		914.4
(-0.7)
P 		830
C 721.3
(-2.7)
P 		809.6
(-1.7)
P 		1232.4
(3)
P 		729.2
(-2.6)
P 		697.4
(-3)
P 		1028.1
(0.7)
P 		535
avr 753.9
(-2) 		886.7
(-0.8) 		1335.2
(3) 		838
(-1.2) 		631.9
(-3) 		934.4
(-0.4) 		703
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 293
1 2 3 4 range
A 292.3
(3)
P 		230.5
(1.4)
P 		193.8
(0.4)
P 		62.1
(-3)
P 		230
B 162
(-0.7)
P 		267.7
(3)
P 		187.2
(0.1)
P 		98.4
(-3)
P 		169
C 287.3
(1.5)
P 		241.2
(0.5)
P 		354.7
(3)
P 		82.3
(-3)
P 		272
avr 247.2
(3) 		246.5
(3) 		245.2
(2.9) 		80.9
(-3) 		166

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 205
1 2 3 4 5 6 range
A 142.3
(-3)
P 		324
(3)
P 		292.7
(2)
P 		230.8
(-0.1)
P 		180.9
(-1.7)
P 		209.5
(-0.8)
P 		182
B 267.5
(-1.1)
P 		312.7
(1.2)
P 		347.1
(3)
P 		231.8
(-3)
P 		243.3
(-2.4)
P 		341
(2.7)
P 		115
C 267.4
(0.7)
P 		247
(0)
P 		313.7
(2.1)
P 		146.6
(-3)
P 		225.7
(-0.6)
P 		344.2
(3)
P 		198
avr 225.7
(-1.8) 		294.6
(1.8) 		317.8
(3) 		203.1
(-3) 		216.6
(-2.3) 		298.2
(2) 		115
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191240_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2941
Sequence Type Exemplar sequence
Representative Public ID F28C6.8 NCBI
Target Description F28C6.8 /REP_DB=WormBase Gene ID /WP=CE09746 /TR=Q93622 /GB=CAA92677.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=MAP kinase phosphatase like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063425(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063425 NCBI Caenorhabditis elegans F28C6.8 (F28C6.8) mRNA, complete cds. 11/11 None
SNAP00000038096 ENSEMBL cdna:SNAP chromosome:CEL140:II:8606771:8607565:1 11/11 None
GENEFINDER00000038101 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8606771:8607565:1 11/11 None
F28C6.8 ENSEMBL cdna:known chromosome:CEL140:II:8606771:8607673:1 gene:F28C6.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8606884-8607562(+) 100.0 100.0
Public Domain and Genome References
Gene Title MAP kinase phosphatase like
Ensembl F28C6.8
Entrez Gene 174381 Entrez gene
SwissProt Q93622 EMBL-EBI
WormBase CE09746 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6470 protein amino acid dephosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4721 phosphoprotein phosphatase activity inferred from electronic annotation QuickGO AmiGO
8138 protein tyrosine/serine/threonine phosphatase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAE57567 Hypothetical protein CBG00545 [Caenorhabditis briggsae] 4.0E-94
blast CAA92677 Hypothetical protein F28C6.8 [Caenorhabditis elegans] ref|NP_495826.1| F28C6.8 [Caenorhabditis elegans] 3.0E-78
blast CAA92677 Hypothetical protein F28C6.8 [Caenorhabditis elegans] ref|NP_495826.1| F28C6.8 [Caenorhabditis elegans] 7.0E-78
blast CAE57567 Hypothetical protein CBG00545 [Caenorhabditis briggsae] 8.0E-72
blast XP_692228 PREDICTED: similar to PTPMT1 protein [Danio rerio] 1.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR000340 EMBL-EBI Dual specificity protein phosphatase 1.3E-46
Pfam IPR000340 EMBL-EBI Dual specificity protein phosphatase 1.3E-46
Sequence
Target Sequence 
>C. ELEGANS:191240_AT
ggtggagtcgtatgttgtactgaagaattcgagttaaaagctgcgatgaatgcaatgaga
gaagttgattggaagaatgaaggtgtcgagttttttgctgtgccgatgaaagattttacg
ggaactgcgccaagagcagagatcaacgaagcggtggaatttattgagagtgtcgcctcg
aaaggaaaaactgtttatgtgcactgcaaagctggaagaactcgaagtgccacagtagca
acttgctatttgatgaaatctcgaaattggatgtccaacgttgcatgggaatttctgaag
gacaaacgacaccaagtacttcttcgaaatgctcactggagaactgtcaacgagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTGGAGTCGTATGTTGTACTGAAG 300 499 61 Antisense
AGGTGTCGAGTTTTTTGCTGTGCCG 643 55 141 Antisense
GATTTTACGGGAACTGCGCCAAGAG 221 429 172 Antisense
TATTGAGAGTGTCGCCTCGAAAGGA 709 663 222 Antisense
GAAAAACTGTTTATGTGCACTGCAA 550 355 245 Antisense
TGCACTGCAAAGCTGGAAGAACTCG 709 575 260 Antisense
GAAGTGCCACAGTAGCAACTTGCTA 269 335 284 Antisense
ATTGGATGTCCAACGTTGCATGGGA 700 3 326 Antisense
GACAAACGACACCAAGTACTTCTTC 355 365 361 Antisense
GTACTTCTTCGAAATGCTCACTGGA 701 459 376 Antisense
GCTCACTGGAGAACTGTCAACGAGT 348 301 391 Antisense
Sequence Source Affymetrix Proprietary Database