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[191230_at] 7TM chemoreceptor, srh family

Description F09G2.7 /REP_DB=WormBase Gene ID /WP=CE09302 /GEN=srh-83 /TR=O17400 /GB=AAB71319.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster 6grp_total > 85      4grp_total > 17      6grp_cgc > 77      6grp_cgc_sub > 04_05_06      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 131
1 2 3 4 range
A 3.9
(-3)
A 		11
(-2.7)
A 		37.6
(-1.5)
A 		134.7
(3)
A 		131
B 51.3
(-0.2)
A 		69.6
(3)
A 		52.2
(-0.1)
A 		35.8
(-3)
A 		34
C 7.3
(-3)
A 		37.4
(-1.4)
A 		29.5
(-1.8)
A 		121.3
(3)
P 		114
avr 20.8
(-3) 		39.3
(-1.5) 		39.8
(-1.5) 		97.3
(3) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 63.9
(3)
A 		6.7
(-3)
A 		49.7
(1.5)
A 		21.7
(-1.4)
A 		46.5
(1.2)
A 		53.2
(1.9)
A 		57
B 14.5
(-2.4)
A 		47.4
(1.1)
A 		8.5
(-3)
A 		66
(3)
P 		34.1
(-0.3)
A 		55.5
(1.9)
A 		58
C 46
(-0.9)
A 		46.6
(-0.8)
A 		30.8
(-3)
A 		74.6
(3)
A 		33.3
(-2.7)
A 		53.8
(0.2)
A 		44
avr 41.5
(-0.1) 		33.6
(-2) 		29.7
(-3) 		54.1
(3) 		38
(-1) 		54.2
(3) 		25
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 19
1 2 3 4 range
A 1.4
(-3)
A 		2.4
(-2.7)
A 		9.8
(-0.2)
A 		19.7
(3)
A 		18
B 16.4
(2.5)
A 		17.2
(3)
A 		12.1
(-0.2)
A 		7.6
(-3)
A 		10
C 2.4
(-3)
A 		10.8
(-0.2)
A 		10.3
(-0.4)
A 		20.4
(3)
P 		18
avr 6.7
(-3) 		10.1
(-0.8) 		10.7
(-0.4) 		15.9
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 11.8
(1.9)
A 		2.4
(-3)
A 		10.8
(1.3)
A 		5.5
(-1.4)
A 		14
(3)
A 		12.9
(2.4)
A 		12
B 5.1
(-2)
A 		15.8
(1.4)
A 		2.1
(-3)
A 		17.5
(2)
P 		13.9
(0.8)
A 		20.7
(3)
A 		19
C 17.1
(2.5)
A 		14.2
(0.8)
A 		7.8
(-3)
A 		15
(1.2)
A 		10.8
(-1.2)
A 		18
(3)
A 		10
avr 11.3
(-0.4) 		10.8
(-0.7) 		6.9
(-3) 		12.7
(0.4) 		12.9
(0.5) 		17.2
(3) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191230_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11385
Sequence Type Exemplar sequence
Representative Public ID F09G2.7 NCBI
Target Description F09G2.7 /REP_DB=WormBase Gene ID /WP=CE09302 /GEN=srh-83 /TR=O17400 /GB=AAB71319.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072418(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072418 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-83) (srh-83) mRNA, complete cds. 11/11 None
F09G2.7 ENSEMBL cdna:known chromosome:CEL140:V:7167102:7168487:1 gene:F09G2.7 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000025951 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:7167102-7168488(+) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, srh family
Gene Symbol srh-83
Ensembl F09G2.7
Entrez Gene 191875 Entrez gene
SwissProt O17400 EMBL-EBI
WormBase CE09302 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_504819 Serpentine Receptor, class H family member (srh-83) [Caenorhabditis elegans] gb|AAB71319.1| Serpentine receptor, class h protein 83 [Caenorhabditis elegans] 0.0
blast AAN73869 Serpentine receptor, class h protein 88 [Caenorhabditis elegans] ref|NP_503807.1| Serpentine Receptor, class H family member (srh-88) [Caenorhabditis elegans] 1.0E-127
blast AAG24157 Serpentine receptor, class h protein 87 [Caenorhabditis elegans] ref|NP_503800.1| Serpentine Receptor, class H family member (srh-87) [Caenorhabditis elegans] 1.0E-125
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504819.1 7 30-52,69-91,101-123,144-163,209-231,251-273,288-310
F09G2.7 7 30-52,69-91,101-123,144-163,209-231,251-273,288-310
Sequence
Target Sequence 
>C. ELEGANS:191230_AT
ttggttcgttatctatcgggtctaatatattctatgtctttactattcttcataccagaa
gatcaaaattccgcactactccaagttctaaccaaaataccatgcccgtctcaagaattt
ttcacggcttccgatgtttttgtattgtgtatcgatgagaactacgtcacgttcctggca
ctattcacagggattggtgtcttgacagagatagctcaaattgtatttttcttagcgtgt
tgtgtatattatttatttgtctcggtacgatcattcgcttctaaaaagacacgacaaatg
caagttaaatattttgcaagtataattttacaaatatcaattccaatgggattcatgatg
cctaccgttctctacatattcctttctttatcatacaagttttataatcaagctctaact
aacttatcaatacttcatgcatcacttcacgacttgttatccacatttatggtactttta
attcatgctccataccggcatttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGTTCGTTATCTATCGGGTCTAA 246 693 448 Antisense
ATGTCTTTACTATTCTTCATACCAG 148 49 481 Antisense
AAGATCAAAATTCCGCACTACTCCA 461 155 506 Antisense
ATACCATGCCCGTCTCAAGAATTTT 311 23 544 Antisense
CACGGCTTCCGATGTTTTTGTATTG 42 159 570 Antisense
TGAGAACTACGTCACGTTCCTGGCA 167 567 603 Antisense
TTGTCTCGGTACGATCATTCGCTTC 116 701 704 Antisense
AATGGGATTCATGATGCCTACCGTT 480 163 792 Antisense
TTCTCTACATATTCCTTTCTTTATC 307 693 815 Antisense
TGCATCACTTCACGACTTGTTATCC 334 579 885 Antisense
AATTCATGCTCCATACCGGCATTTT 40 177 927 Antisense
Sequence Source Affymetrix Proprietary Database