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[191227_s_at] Leucine aminopeptidase

Description ZK353.6 /REP_DB=WormBase Gene ID /WP=CE00390 /TR=SW:P34629 /GB=AAA28198.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Leucine aminopeptidase
Cluster 6grp_highest_10_10 > 38      6grp_high1_10_10 > 83      4grp_highest_8_8 > 34      4grp_high_8_8 > 49      6grp_highest_nonorm > 65      6grp_total > 73      4grp_total > 62      6grp_highest_sub > 04      6grp_cgc > 76      6grp_high1_sub > 04      6grp_cgc_sub > 03_04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 511
1 2 3 4 range
A 1343.7
(3)
P 		1292
(2.3)
P 		1158
(0.4)
P 		918.5
(-3)
P 		425
B 1429.9
(3)
P 		1082.7
(-3)
P 		1297.5
(0.7)
P 		1106.6
(-2.6)
P 		347
C 1268.3
(3)
P 		1077.4
(-3)
P 		1191
(0.6)
P 		1122.6
(-1.6)
P 		191
avr 1347.3
(3) 		1150.7
(-1) 		1215.5
(0.3) 		1049.2
(-3) 		298

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 570
1 2 3 4 5 6 range
A 794.3
(-3)
P 		979.2
(-0.7)
P 		1133.8
(1.2)
P 		1276.3
(3)
P 		1172.3
(1.7)
P 		1071.4
(0.4)
P 		482
B 897.4
(-3)
P 		915.4
(-2.8)
P 		1359
(3)
P 		1337.1
(2.7)
P 		1338.1
(2.7)
P 		1140.1
(0.2)
P 		462
C 827.5
(-3)
P 		896.5
(-2.2)
P 		1364.4
(3)
P 		1301
(2.3)
P 		1335.8
(2.7)
P 		1176.9
(0.9)
P 		537
avr 839.7
(-3) 		930.4
(-1.8) 		1285.7
(2.8) 		1304.8
(3) 		1282.1
(2.7) 		1129.5
(0.7) 		465
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 355
1 2 3 4 range
A 489.2
(3)
P 		280.5
(-0.5)
P 		302.7
(-0.2)
P 		134.4
(-3)
P 		355
B 456
(3)
P 		267.4
(-2.1)
P 		300.7
(-1.2)
P 		235.8
(-3)
P 		220
C 419.9
(3)
P 		309.8
(0.1)
P 		415.1
(2.9)
P 		189.3
(-3)
P 		231
avr 455
(3) 		285.9
(-0.8) 		339.5
(0.4) 		186.5
(-3) 		269

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 397
1 2 3 4 5 6 range
A 146.4
(-3)
P 		348.2
(2.8)
P 		246.9
(-0.1)
P 		323.7
(2.1)
P 		353.8
(3)
P 		260.9
(0.3)
P 		207
B 312.2
(-2.8)
P 		304.7
(-3)
P 		330
(-2.4)
P 		354.1
(-1.8)
P 		543.6
(3)
P 		425.1
(0)
P 		239
C 306.7
(-1.4)
P 		273.5
(-2.6)
P 		347.3
(0)
P 		261.6
(-3)
P 		432.2
(3)
P 		394
(1.7)
P 		171
avr 255.1
(-3) 		308.8
(-1.3) 		308.1
(-1.3) 		313.1
(-1.1) 		443.2
(3) 		360
(0.3) 		188
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191227_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6056
Sequence Type Exemplar sequence
Representative Public ID ZK353.6 NCBI
Target Description ZK353.6 /REP_DB=WormBase Gene ID /WP=CE00390 /TR=SW:P34629 /GB=AAA28198.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Leucine aminopeptidase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066453(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066453 NCBI Caenorhabditis elegans Leucine AminoPeptidase family member (lap-1) (lap-1) mRNA, complete cds. 11/11 None
SNAP00000044095 ENSEMBL cdna:SNAP chromosome:CEL140:III:8399213:8401824:-1 11/11 None
GENEFINDER00000044107 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8399213:8401824:-1 11/11 None
ZK353.6.1 ENSEMBL cdna:known chromosome:CEL140:III:8399119:8401843:-1 gene:ZK353.6 11/11 None
ZK353.6.2 ENSEMBL cdna:known chromosome:CEL140:III:8399119:8401845:-1 gene:ZK353.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8399208-8401820(-) 100.0 100.0
Public Domain and Genome References
Gene Title Leucine aminopeptidase
Gene Symbol lap-1
Ensembl ZK353.6
Entrez Gene 176185 Entrez gene
SwissProt P34629 EMBL-EBI
WormBase CE00390 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1636125_A_AT granny smith dm
DROSGENOME1:153792_AT granny smith dm
CHICKEN:GGA.8283.1.S1_AT similar to aminopeptidase-like 1 gga
HG-U133_PLUS_2:218822_S_AT aminopeptidase-like 1 hs
HG-U133A:218822_S_AT aminopeptidase-like 1 hs
HG-U133A_2:218822_S_AT aminopeptidase-like 1 hs
HG-U133A_2:89476_R_AT aminopeptidase-like 1 hs
HG-U133_PLUS_2:89476_R_AT aminopeptidase-like 1 hs
HG-U133A:89476_R_AT aminopeptidase-like 1 hs
HG-U95E:89476_R_AT aminopeptidase-like 1 hs
HU35KSUBC:RC_AA477424_F_AT aminopeptidase-like 1 hs
HU35KSUBD:RC_AA290862_F_AT aminopeptidase-like 1 hs
U133_X3P:G13375916_3P_S_AT aminopeptidase-like 1 hs
U133_X3P:G13375916_3P_A_AT aminopeptidase-like 1 hs
U133_X3P:HS.71746.0.S2_3P_AT aminopeptidase-like 1 hs
HG-U133A:218821_AT aminopeptidase-like 1 hs
HG-U133A_2:218821_AT aminopeptidase-like 1 hs
HG-U133_PLUS_2:218821_AT aminopeptidase-like 1 hs
HG-U95B:46662_AT aminopeptidase-like 1 hs
HG-U95D:69524_AT aminopeptidase-like 1 hs
HG-U95E:89473_S_AT aminopeptidase-like 1 hs
U133_X3P:HS.135078.0.A1_3P_AT Aminopeptidase-like 1 hs
HG-U133_PLUS_2:235033_AT Aminopeptidase-like 1 hs
HG-U133B:235033_AT Aminopeptidase-like 1 hs
HG-U95E:76841_AT Aminopeptidase-like 1 hs
HG-U95C:61771_AT Aminopeptidase-like 1 hs
HG-U95E:77823_AT Aminopeptidase-like 1 hs
MU11KSUBA:AA032720_S_AT aminopeptidase-like 1 mm
MG-U74AV2:160732_AT aminopeptidase-like 1 mm
MOE430A:1434507_AT aminopeptidase-like 1 mm
MOUSE430A_2:1434507_AT aminopeptidase-like 1 mm
MOUSE430_2:1434507_AT aminopeptidase-like 1 mm
MU19KSUBB:TC27588_AT Aminopeptidase-like 1, mRNA (cDNA clone IMAGE:3598171) mm
RG-U34B:RC_AA955976_AT aminopeptidase-like 1 (predicted) rn
RAE230A:1373570_AT aminopeptidase-like 1 (predicted) rn
RAT230_2:1373570_AT aminopeptidase-like 1 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4177 aminopeptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA28198 Leucine aminopeptidase protein 1 [Caenorhabditis elegans] ref|NP_498854.1| Leucine AminoPeptidase family member (lap-1) [Caenorhabditis elegans] sp|P34629|AMPL_CAEEL Leucine aminopeptidase 0.0
blast CAE71265 Hypothetical protein CBG18147 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000819 EMBL-EBI Peptidase M17, cytosol aminopeptidase, C-terminal 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:191227_S_AT
agcttactctgctcttgtcaaacacggtttttcacaaactctccatgcttgtctctgtat
tgttgagaataatgtcagcccaattgcaaacaagcctgatgatattatcaaaatgttgag
tggaaagaccgtcgaaatcaacaatactgacgcagaaggtcgtttgattcttgctgacgg
agttttctatgcaaaagagactctcaaagcaacaactatattcgatatggctactctgac
cggtgcacaagcgtggctttcaggacgtcttcacggagctgctatgacaaatgatgaaca
gttggagaacgaaatcatcaaagctggaaaggcttcaggagacttggttgccccaatgct
ttttgccccggatcttttctttggtgacttgaaatcttctattgctgacatgaaaaattc
gaatctcggaaaaatggatggaccaccatctgcagtcgctggcctctttattggtgctca
cattggttttggcgaaggactacgttggcttcatcttgatatcgctgctcccgctgaagt
tggagatcgtggta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCTTACTCTGCTCTTGTCAAACAC 337 79 864 Antisense
TTTCACAAACTCTCCATGCTTGTCT 672 671 893 Antisense
AAGACCGTCGAAATCAACAATACTG 341 151 988 Antisense
GAAGGTCGTTTGATTCTTGCTGACG 175 333 1018 Antisense
TTCTTGCTGACGGAGTTTTCTATGC 260 689 1031 Antisense
TTCGATATGGCTACTCTGACCGGTG 704 691 1084 Antisense
CAAGCGTGGCTTTCAGGACGTCTTC 495 185 1111 Antisense
ACGTCTTCACGGAGCTGCTATGACA 96 95 1128 Antisense
TCAGGAGACTTGGTTGCCCCAATGC 423 621 1198 Antisense
CCGGATCTTTTCTTTGGTGACTTGA 506 263 1231 Antisense
CCCGCTGAAGTTGGAGATCGTGGTA 573 259 1393 Antisense
Sequence Source Affymetrix Proprietary Database