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[191225_at]

Description T05A1.1 /REP_DB=WormBase Gene ID /WP=CE03621 /TR=Q22188 /GB=CAA92481.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster 6grp_highest_10_10 > 61      6grp_high1_10_10 > 27      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 70      6grp_total > 27      4grp_total > 13      6grp_highest_sub > De      6grp_cgc > 05      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 487
1 2 3 4 range
A 444.2
(2.2)
P 		190.9
(-2.3)
P 		153.5
(-3)
P 		491.7
(3)
P 		338
B 407.8
(-0.1)
P 		205.2
(-2.7)
P 		184.7
(-3)
P 		639.8
(3)
P 		455
C 408.4
(-0.4)
P 		300.2
(-2)
P 		228.9
(-3)
P 		640.9
(3)
P 		412
avr 420.1
(0.5) 		232.1
(-2.4) 		189
(-3) 		590.8
(3) 		402

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 370
1 2 3 4 5 6 range
A 531.4
(3)
P 		307
(-1.2)
P 		296
(-1.4)
P 		208.3
(-3)
P 		266.7
(-1.9)
P 		238
(-2.4)
P 		323
B 501.3
(3)
P 		386.2
(0.2)
P 		250.5
(-3)
P 		261.3
(-2.7)
P 		308.5
(-1.6)
P 		334.5
(-1)
P 		251
C 389.9
(2.6)
P 		405.8
(3)
P 		247.3
(-0.9)
P 		161.1
(-3)
P 		201.8
(-2)
P 		286.3
(0.1)
P 		245
avr 474.2
(3) 		366.3
(0.5) 		264.6
(-1.8) 		210.2
(-3) 		259
(-1.9) 		286.3
(-1.3) 		264
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 122
1 2 3 4 range
A 161.7
(3)
P 		41.4
(-2.9)
P 		40.1
(-3)
P 		72
(-1.4)
P 		122
B 130.1
(2.6)
P 		50.7
(-2.5)
P 		42.8
(-3)
P 		136.3
(3)
P 		94
C 135.2
(3)
P 		86.3
(-2.3)
P 		79.8
(-3)
P 		108.1
(0.1)
P 		55
avr 142.3
(3) 		59.5
(-2.6) 		54.2
(-3) 		105.5
(0.5) 		88

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 142
1 2 3 4 5 6 range
A 98
(1.8)
P 		109.2
(3)
P 		64.5
(-1.8)
P 		52.8
(-3)
P 		80.5
(-0.1)
P 		57.9
(-2.5)
P 		56
B 174.4
(3)
P 		128.6
(0.6)
P 		60.8
(-3)
P 		69.2
(-2.6)
P 		125.3
(0.4)
P 		124.7
(0.4)
P 		114
C 144.5
(3)
P 		123.8
(1.9)
P 		62.9
(-1.4)
P 		32.4
(-3)
P 		65.3
(-1.2)
P 		95.8
(0.4)
P 		112
avr 139
(3) 		120.5
(1.7) 		62.7
(-2.2) 		51.5
(-3) 		90.4
(-0.3) 		92.8
(-0.2) 		88
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191225_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9707
Sequence Type Exemplar sequence
Representative Public ID T05A1.1 NCBI
Target Description T05A1.1 /REP_DB=WormBase Gene ID /WP=CE03621 /TR=Q22188 /GB=CAA92481.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069300(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069300 NCBI Caenorhabditis elegans Neuropeptide Receptor family member (npr-2) (npr-2) mRNA, complete cds. 11/11 None
GENEFINDER00000033950 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9568486:9571926:-1 9/11 None
T05A1.1a ENSEMBL cdna:known chromosome:CEL140:IV:9568810:9580045:-1 gene:T05A1.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_182289 8/11 Cross Hyb Matching Probes None
SNAP00000033945 4/11 Cross Hyb Matching Probes None
T05A1.1b.1 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177790 2 1 NM_069300 191225_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9568943-9571932(-) 100.0 100.0
Public Domain and Genome References
Ensembl T05A1.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD59155 Hypothetical protein T05A1.1a [Caenorhabditis elegans] emb|CAD59147.1| Hypothetical protein T05A1.1a [Caenorhabditis elegans] ref|NP_501701.2| Neuropeptide Receptor family member (npr-2) [Caenorhabditis elegans] 0.0
blast CAA92481 Hypothetical protein T05A1.1b [Caenorhabditis elegans] ref|NP_872089.1| Neuropeptide Receptor family member (npr-2) [Caenorhabditis elegans] 0.0
blast CAE72782 Hypothetical protein CBG20037 [Caenorhabditis briggsae] 1.0E-112
blast CAE72782 Hypothetical protein CBG20037 [Caenorhabditis briggsae] 1.0E-111
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 5.2E-56
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 3.5E-55
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501701.2 7 71-93,106-128,143-165,186-205,234-256,319-341,361-383
T05A1.1a 7 71-93,106-128,143-165,186-205,234-256,319-341,361-383
GENEFINDER00000033950 7 28-50,63-85,100-122,143-162,191-213,283-305,325-347
Sequence
Target Sequence 
>C. ELEGANS:191225_AT
gagagtactgttctgagcactggccattagcagaagtacgaaagggatatacttttttgg
tgttaatcactcaatttttgtttccttttgctacgatggctttttgctattataatattt
tttcaagactcagacaacgagtggaaacgaaactgaagaaattatcagaaagatctcaac
ttttggagaatagtacaacatgtggaaccactaaccacattgttagcattaacgcggtaa
gtaagggtcacattgtatatcaaggtaacaatacatttctagaaattacatttttaaatg
tgttttttgtgctaatgtacggaagcagttcaaaatggtctggaaaacaaacaaagatta
gctgttcttgctcaacaaagaaggacaactactattttatcgtgcatgaggtggaagtaa
atccccgaaaccagctgacatcaagcaaacttcattcattcgaatgccgaatagtggtgc
gccaagtcaatcttcctacctgtgattttgtttcaaattataccgtttttcattgcattt
ttcaaaagaattgctttcaacatcacgtgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAGTACTGTTCTGAGCACTGGCC 637 395 530 Antisense
GAGCACTGGCCATTAGCAGAAGTAC 57 389 544 Antisense
TAATCACTCAATTTTTGTTTCCTTT 94 635 593 Antisense
GTTTCCTTTTGCTACGATGGCTTTT 59 451 609 Antisense
GACAACTACTATTTTATCGTGCATG 524 365 913 Antisense
TCCCCGAAACCAGCTGACATCAAGC 537 595 951 Antisense
TCGAATGCCGAATAGTGGTGCGCCA 90 601 989 Antisense
TAGTGGTGCGCCAAGTCAATCTTCC 168 655 1001 Antisense
TCTTCCTACCTGTGATTTTGTTTCA 521 615 1020 Antisense
ATTATACCGTTTTTCATTGCATTTT 530 13 1046 Antisense
AGAATTGCTTTCAACATCACGTGTT 652 75 1076 Antisense
Sequence Source Affymetrix Proprietary Database