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[191221_at] glutathione S-transferase

Description R07B1.4 /REP_DB=WormBase Gene ID /WP=CE01630 /TR=SW:Q09607 /GB=CAA88541.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=glutathione transferase
Cluster 6grp_highest_10_10 > 24      6grp_high1_10_10 > 31      4grp_highest_8_8 > 24      4grp_high_8_8 > 52      6grp_highest_nonorm > 25      6grp_total > 31      4grp_total > 60      6grp_cgc > 81      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3619
1 2 3 4 range
A 3447.3
(1.1)
P 		4583.5
(3)
P 		4114.8
(2.2)
P 		987.2
(-3)
P 		3596
B 3808.6
(2.6)
P 		3984.7
(3)
P 		3840
(2.7)
P 		969.7
(-3)
P 		3015
C 3747.5
(2.5)
P 		3941.9
(2.8)
P 		4026.9
(3)
P 		964.1
(-3)
P 		3063
avr 3667.8
(2.1) 		4170
(3) 		3993.9
(2.7) 		973.7
(-3) 		3196

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3997
1 2 3 4 5 6 range
A 4178.4
(0.2)
P 		4339.2
(0.4)
P 		6029.3
(3)
P 		3611.2
(-0.7)
P 		2865.6
(-1.8)
P 		2097
(-3)
P 		3932
B 4108.4
(-0.3)
P 		4760.6
(0.8)
P 		6093.6
(3)
P 		3757.3
(-0.9)
P 		3370.8
(-1.5)
P 		2485.1
(-3)
P 		3609
C 3221.1
(-1)
P 		5025.6
(3)
P 		4475.4
(1.8)
P 		4279.1
(1.3)
P 		2340.8
(-3)
P 		2777.4
(-2)
P 		2685
avr 3836
(-0.3) 		4708.5
(1.4) 		5532.8
(3) 		3882.5
(-0.2) 		2859.1
(-2.2) 		2453.2
(-3) 		3080
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1259
1 2 3 4 range
A 1255.1
(3)
P 		995
(1.6)
P 		1075.7
(2)
P 		144.5
(-3)
P 		1111
B 1214.5
(3)
P 		984.1
(1.6)
P 		889.8
(1.1)
P 		206.7
(-3)
P 		1008
C 1240.5
(2.2)
P 		1133.3
(1.7)
P 		1403.5
(3)
P 		162.6
(-3)
P 		1241
avr 1236.7
(3) 		1037.5
(1.9) 		1123
(2.4) 		171.3
(-3) 		1065

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1074
1 2 3 4 5 6 range
A 770.2
(-1.5)
P 		1543.2
(3)
P 		1313.1
(1.7)
P 		915.9
(-0.6)
P 		864.7
(-0.9)
P 		510.6
(-3)
P 		1033
B 1429.5
(1.6)
P 		1584.6
(3)
P 		1479.7
(2)
P 		995.2
(-2.4)
P 		1369.5
(1)
P 		926.7
(-3)
P 		658
C 1193.9
(0.4)
P 		1533.5
(3)
P 		1139.1
(-0)
P 		860.5
(-2.2)
P 		757.4
(-3)
P 		929.8
(-1.7)
P 		776
avr 1131.2
(-0.3) 		1553.8
(3) 		1310.6
(1.1) 		923.9
(-1.9) 		997.2
(-1.4) 		789
(-3) 		765
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191221_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17052
Sequence Type Exemplar sequence
Representative Public ID R07B1.4 NCBI
Target Description R07B1.4 /REP_DB=WormBase Gene ID /WP=CE01630 /TR=SW:Q09607 /GB=CAA88541.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=glutathione transferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077251(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077251 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-36) (gst-36) mRNA, complete cds. 10/11 None
SNAP00000020690 ENSEMBL cdna:SNAP chromosome:CEL140:X:9861696:9862886:-1 11/11 None
GENEFINDER00000020712 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9861696:9862886:-1 11/11 None
R07B1.4 ENSEMBL cdna:known chromosome:CEL140:X:9861607:9862886:-1 gene:R07B1.4 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9861695-9862886(-) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-36
Ensembl R07B1.4
Entrez Gene 187645 Entrez gene
SwissProt Q09607 EMBL-EBI
WormBase CE30562 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast T23994 hypothetical protein R07B1.4 - Caenorhabditis elegans 1.0E-120
blast CAA88541 Hypothetical protein R07B1.4 [Caenorhabditis elegans] ref|NP_509652.2| Glutathione S-Transferase family member (gst-36) [Caenorhabditis elegans] sp|Q09607|GST36_CAEEL Probable glutathione S-transferase gst-36 (GST class-sigma) 1.0E-110
blast T23994 hypothetical protein R07B1.4 - Caenorhabditis elegans 1.0E-105
blast CAA88541 Hypothetical protein R07B1.4 [Caenorhabditis elegans] ref|NP_509652.2| Glutathione S-Transferase family member (gst-36) [Caenorhabditis elegans] sp|Q09607|GST36_CAEEL Probable glutathione S-transferase gst-36 (GST class-sigma) 1.0E-105
blast CAE58723 Hypothetical protein CBG01908 [Caenorhabditis briggsae] 1.0E-96
Protein Domains
Database ID Description E-Value
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 1.8E-9
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 4.8E-18
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 1.8E-9
Sequence
Target Sequence 
>C. ELEGANS:191221_AT
gctcttccaccttgctgatgagaaatttgatgatgagcgctttggaatggagcagtgggg
agttttgaaatccgagatgccgctcggacaggttcctgttcttgaaattgatggtgtcaa
aattagccaaactacagcaatcgctcgttatctcggacatcagttccgtaagttgctaaa
gaaaatgaacatgttcgaaaatcatattgccacgttatcagaccgcgcgggaaccaatgc
tgtggactgtgctcgcttggatatgattgctgaagttattcaagaattcatgagctcttc
cggaatgggaaagttttcccgagtcttgctcggaatgattcaggctaacaaagaacaatt
cttcaaggagaatgttctcccagatgttgagaaatatgctccaattgtcgagaagttcct
cttggaaaatggaaataatggcctgctccttggagatcgcgaaacctgggttgatgtttt
tgcggctgaaagtttctccaagctcattgattacggaagcccagatgctcttgatgccta
ccctcacattttggctcttatcaaccgtgtattcaaccatccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCTTCCACCTTGCTGATGAGAAA 264 301 72 Antisense
CGCTCGGACAGGTTCCTGTTCTTGA 416 255 152 Antisense
TACAGCAATCGCTCGTTATCTCGGA 631 641 204 Antisense
TCATATTGCCACGTTATCAGACCGC 356 619 273 Antisense
GCGCGGGAACCAATGCTGTGGACTG 674 285 296 Antisense
TGGACTGTGCTCGCTTGGATATGAT 23 549 314 Antisense
AAATAATGGCCTGCTCCTTGGAGAT 487 109 504 Antisense
GGCTGAAAGTTTCTCCAAGCTCATT 105 541 555 Antisense
GATTACGGAAGCCCAGATGCTCTTG 428 429 580 Antisense
CACATTTTGGCTCTTATCAACCGTG 432 195 616 Antisense
ATCAACCGTGTATTCAACCATCCAA 195 27 631 Antisense
Sequence Source Affymetrix Proprietary Database