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[191220_at]

Description C07A9.3 /REP_DB=WormBase Gene ID /WP=CE23524 /TR=SW:P34314 /GB=CAA82347.2 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 25      4grp_highest_8_8 > 45      4grp_high_8_8 > 09      6grp_highest_nonorm > 51      6grp_total > 01      4grp_total > 23      6grp_highest_sub > 01_02      6grp_cgc > 04      6grp_high1_sub > 01_02      6grp_cgc_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 331
1 2 3 4 range
A 776.7
(1.5)
P 		582.7
(-3)
P 		613.1
(-2.3)
P 		839.6
(3)
P 		257
B 763.7
(2.9)
P 		563
(-3)
P 		600.4
(-1.9)
P 		768
(3)
P 		205
C 893.8
(3)
P 		614.8
(-3)
P 		628.6
(-2.7)
P 		679.9
(-1.6)
P 		279
avr 811.4
(3) 		586.8
(-3) 		614
(-2.3) 		762.5
(1.7) 		225

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 438
1 2 3 4 5 6 range
A 602.2
(-0.1)
P 		768.7
(3)
P 		444.7
(-3)
P 		477.5
(-2.4)
P 		670
(1.2)
P 		673.2
(1.2)
P 		324
B 669.3
(0.5)
P 		832.4
(3)
P 		468.1
(-2.6)
P 		441.5
(-3)
P 		610.2
(-0.4)
P 		519.7
(-1.8)
P 		391
C 803.9
(3)
P 		784.9
(2.7)
P 		552.6
(-0.7)
P 		394.1
(-3)
P 		553.6
(-0.7)
P 		444.8
(-2.3)
P 		410
avr 691.8
(1.3) 		795.3
(3) 		488.5
(-2.1) 		437.7
(-3) 		611.3
(-0.1) 		545.9
(-1.2) 		358
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 181
1 2 3 4 range
A 282.8
(3)
P 		126.5
(-2.9)
P 		160.3
(-1.6)
P 		122.9
(-3)
P 		160
B 243.5
(3)
P 		139.1
(-3)
P 		139.1
(-3)
P 		163.7
(-1.6)
P 		104
C 295.9
(3)
P 		176.8
(-0.9)
P 		219.1
(0.5)
P 		114.6
(-3)
P 		181
avr 274.1
(3) 		147.5
(-2.4) 		172.8
(-1.3) 		133.7
(-3) 		140

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 219
1 2 3 4 5 6 range
A 111
(-2.5)
P 		273.4
(3)
P 		96.8
(-3)
P 		121.1
(-2.2)
P 		202.2
(0.6)
P 		163.9
(-0.7)
P 		177
B 232.9
(1.4)
P 		277.1
(3)
P 		113.7
(-3)
P 		116.9
(-2.9)
P 		247.9
(1.9)
P 		193.8
(-0.1)
P 		163
C 298
(3)
P 		239.5
(1.4)
P 		140.6
(-1.3)
P 		79.3
(-3)
P 		179.1
(-0.3)
P 		148.9
(-1.1)
P 		219
avr 214
(1.1) 		263.3
(3) 		117
(-2.6) 		105.8
(-3) 		209.7
(1) 		168.9
(-0.6) 		158
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191220_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6869
Sequence Type Exemplar sequence
Representative Public ID C07A9.3 NCBI
Target Description C07A9.3 /REP_DB=WormBase Gene ID /WP=CE23524 /TR=SW:P34314 /GB=CAA82347.2 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066744(11), NM_181913(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066744 NCBI Caenorhabditis elegans Tousled-Like Kinase family member (tlk-1) (tlk-1) mRNA, complete cds. 11/11 None
NM_181913 NCBI Caenorhabditis elegans Tousled-Like Kinase family member (tlk-1) (tlk-1) mRNA, complete cds. 9/11 None
GENEFINDER00000039342 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9699415:9709294:1 11/11 None
SNAP00000039331 ENSEMBL cdna:SNAP chromosome:CEL140:III:9704578:9709294:1 11/11 None
C07A9.3a ENSEMBL cdna:known chromosome:CEL140:III:9699777:9710192:1 gene:C07A9.3 11/11 A 
C07A9.3b ENSEMBL cdna:known chromosome:CEL140:III:9699890:9710164:1 gene:C07A9.3 9/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9697292-9709290(+) 97.24 100.0
Public Domain and Genome References
Ensembl C07A9.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA82347 Hypothetical protein C07A9.3a [Caenorhabditis elegans] ref|NP_499145.2| Tousled-Like Kinase family member (tlk-1) [Caenorhabditis elegans] gb|AAR18092.1| Tousled-like kinase [Caenorhabditis elegans] 0.0
blast H88562 protein C07A9.3 [imported] - Caenorhabditis elegans 0.0
blast CAD45580 Hypothetical protein C07A9.3b [Caenorhabditis elegans] ref|NP_871642.2| Tousled-Like Kinase family member (tlk-1) [Caenorhabditis elegans] sp|P34314|TLK1_CAEEL Serine/threonine-protein kinase tousled-like 1 (Tousled-like kinase 1) 0.0
Protein Families
Method ID Description E-Value
hanks 2.1.12 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; CMK2 1.0E-61
hanks 2.1.12 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; CMK2 9.0E-62
hanks 2.1.12 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; CMK2 8.0E-62
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 2.4E-58
Pfam IPR000719 EMBL-EBI Protein kinase 2.4E-58
Pfam IPR000719 EMBL-EBI Protein kinase 2.4E-58
Sequence
Target Sequence 
>C. ELEGANS:191220_AT
gtttctgctcttgtctatctgaatgaaaagagcactcccatcattcattatgatctaaag
cctgcgaatattcttctcgagtcaggaaacaccagcggtgcaatcaagatcaccgacttt
gggctgtcgaaaatcatggagggagagagtgacgatcatgacttgggaatcgagctcacc
tcacagttcgctggaacttattggtatcttccacctgagaccttcatcgttccaccacca
aagatcacttgtaaagtcgatgtctggagtattggagtcatcttctatcaatgtatctat
ggaaagaagccgttcggaaatgatctgactcaacaaaagatcctcgaatacaatacgatt
attaatgcaagagaagtgtcattcccaagcaagcctcaggtgagctccgctgctcaagat
ttcataagaagatgcctgcagtacaggaaagaagacagagcagatgttttcgagctggcg
aagcatgagcttttcagaccaagaggagcgataagagcctccgttgccggatccgtaagt
tctccaagcattccacgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTTCTGCTCTTGTCTATCTGAATG 186 451 3115 Antisense
AAAGAGCACTCCCATCATTCATTAT 434 123 3141 Antisense
TGACTTGGGAATCGAGCTCACCTCA 296 571 3273 Antisense
TATTGGTATCTTCCACCTGAGACCT 264 665 3313 Antisense
GTTCCACCACCAAAGATCACTTGTA 319 441 3343 Antisense
GGAGTATTGGAGTCATCTTCTATCA 282 517 3380 Antisense
CATTCCCAAGCAAGCCTCAGGTGAG 197 215 3494 Antisense
GTGAGCTCCGCTGCTCAAGATTTCA 275 483 3514 Antisense
AGGAGCGATAAGAGCCTCCGTTGCC 54 57 3618 Antisense
TCCGTTGCCGGATCCGTAAGTTCTC 244 593 3634 Antisense
CGTAAGTTCTCCAAGCATTCCACGC 248 247 3648 Antisense
Sequence Source Affymetrix Proprietary Database