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[191212_s_at]

Description K04F10.4A /REP_DB=WormBase Gene ID /WP=CE11728 /GEN=bli-4 /TR=O44763 /GB=AAB96753.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=endoprotease
Cluster 6grp_highest_10_10 > 39      6grp_high1_10_10 > 70      4grp_highest_8_8 > 16      4grp_high_8_8 > 52      6grp_highest_nonorm > 74      6grp_total > 90      4grp_total > 59      6grp_highest_sub > 04      6grp_cgc > 96      6grp_high1_sub > 04      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 319
1 2 3 4 range
A 530.8
(0.1)
P 		646.2
(3)
P 		565.3
(1)
P 		408.7
(-3)
P 		238
B 573.8
(0)
P 		596.7
(0.9)
P 		647.4
(3)
P 		500.1
(-3)
P 		147
C 589.2
(1.5)
P 		667.7
(3)
P 		667.1
(3)
P 		349.1
(-3)
P 		319
avr 564.6
(1) 		636.9
(3) 		626.6
(2.7) 		419.3
(-3) 		218

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 335
1 2 3 4 5 6 range
A 710
(0.3)
P 		791.6
(2.6)
P 		589.4
(-3)
P 		764.3
(1.8)
P 		777.8
(2.2)
P 		806
(3)
P 		217
B 716.2
(-2)
P 		751.5
(-0.9)
P 		773.6
(-0.2)
P 		876.9
(3)
P 		684.6
(-3)
P 		736.4
(-1.4)
P 		192
C 888.5
(2.2)
P 		729.8
(-1.4)
P 		660.3
(-3)
P 		924.8
(3)
P 		747.8
(-1)
P 		754.4
(-0.9)
P 		265
avr 771.6
(0.2) 		757.6
(-0.2) 		674.4
(-3) 		855.3
(3) 		736.7
(-0.9) 		765.6
(0) 		181
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 174
1 2 3 4 range
A 193.3
(3)
P 		140.3
(0.6)
P 		147.8
(1)
P 		59.8
(-3)
P 		134
B 183
(3)
P 		147.4
(0.2)
P 		150
(0.4)
P 		106.6
(-3)
P 		76
C 195.1
(1.7)
P 		192
(1.6)
P 		232.5
(3)
P 		58.9
(-3)
P 		174
avr 190.5
(3) 		159.9
(1.4) 		176.8
(2.3) 		75.1
(-3) 		115

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 201
1 2 3 4 5 6 range
A 130.9
(-2.9)
P 		281.5
(3)
P 		128.4
(-3)
P 		193.9
(-0.4)
P 		234.7
(1.2)
P 		196.2
(-0.3)
P 		153
B 249.2
(1.1)
P 		250.2
(1.1)
P 		187.9
(-3)
P 		232.3
(-0)
P 		278.1
(3)
P 		274.6
(2.8)
P 		90
C 329.3
(3)
P 		222.7
(-1)
P 		168.1
(-3)
P 		186
(-2.3)
P 		242
(-0.2)
P 		252.5
(0.1)
P 		161
avr 236.5
(2) 		251.5
(3) 		161.5
(-3) 		204.1
(-0.2) 		251.6
(3) 		241.1
(2.3) 		90
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191212_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1765
Sequence Type Exemplar sequence
Representative Public ID K04F10.4A NCBI
Target Description K04F10.4A /REP_DB=WormBase Gene ID /WP=CE11728 /GEN=bli-4 /TR=O44763 /GB=AAB96753.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=endoprotease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026370(11), NM_001026369(11), NM_001026372(11), NM_001026371(11), NM_001026374(11), NM_001026373(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026370 NCBI Caenorhabditis elegans K04F10.4b (endoprotease) mRNA, complete cds. 11/11 None
NM_001026369 NCBI Caenorhabditis elegans K04F10.4a (endoprotease) mRNA, complete cds. 11/11 None
NM_001026372 NCBI Caenorhabditis elegans K04F10.4d (endoprotease) mRNA, complete cds. 11/11 None
NM_001026371 NCBI Caenorhabditis elegans K04F10.4c (endoprotease) mRNA, complete cds. 11/11 None
NM_001026374 NCBI Caenorhabditis elegans K04F10.4f (endoprotease) mRNA, complete cds. 11/11 None
NM_001026373 NCBI Caenorhabditis elegans K04F10.4e (endoprotease) mRNA, complete cds. 11/11 None
SNAP00000027815 ENSEMBL cdna:SNAP chromosome:CEL140:I:6350572:6353721:1 11/11 None
GENEFINDER00000027829 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6350572:6353721:1 11/11 None
K04F10.4b ENSEMBL cdna:known chromosome:CEL140:I:6349479:6361740:1 gene:K04F10.4 11/11 A 
K04F10.4a.1 ENSEMBL cdna:known chromosome:CEL140:I:6350366:6356621:1 gene:K04F10.4 11/11 A 
K04F10.4a.2 ENSEMBL cdna:known chromosome:CEL140:I:6350366:6356599:1 gene:K04F10.4 11/11 A 
K04F10.4f ENSEMBL cdna:known chromosome:CEL140:I:6350572:6354300:1 gene:K04F10.4 11/11 A 
K04F10.4e ENSEMBL cdna:known chromosome:CEL140:I:6350572:6353982:1 gene:K04F10.4 11/11 A 
K04F10.4d ENSEMBL cdna:known chromosome:CEL140:I:6350572:6364813:1 gene:K04F10.4 11/11 A 
K04F10.4c ENSEMBL cdna:known chromosome:CEL140:I:6350572:6363567:1 gene:K04F10.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000016565 1/11 Cross Hyb Matching Probes None
GENEFINDER00000016575 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6350500-6356397(+) 100.0 100.0
Public Domain and Genome References
Ensembl K04F10.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB96756 Blistered cuticle protein 4, isoform b [Caenorhabditis elegans] ref|NP_001021541.1| K04F10.4b [Caenorhabditis elegans] pir||F87803 protein bli-4B [imported] - Caenorhabditis elegans 0.0
blast CAE67165 Hypothetical protein CBG12593 [Caenorhabditis briggsae] 0.0
blast AAB96754 Blistered cuticle protein 4, isoform d [Caenorhabditis elegans] ref|NP_001021543.1| K04F10.4d [Caenorhabditis elegans] pir||D87803 protein bli-4D [imported] - Caenorhabditis elegans 0.0
blast AAB96753 Blistered cuticle protein 4, isoform a [Caenorhabditis elegans] ref|NP_001021540.1| K04F10.4a [Caenorhabditis elegans] 0.0
blast AAO12410 Blistered cuticle protein 4, isoform f [Caenorhabditis elegans] ref|NP_001021545.1| K04F10.4f [Caenorhabditis elegans] 0.0
blast P51559 Endoprotease bli-4 precursor (Blisterase) (Blistered cuticle protein 4) 0.0
blast AAB96757 Blistered cuticle protein 4, isoform c [Caenorhabditis elegans] ref|NP_001021542.1| K04F10.4c [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002884 EMBL-EBI Peptidase S8B, proprotein convertase, P domain 2.9E-70
Pfam IPR000209 EMBL-EBI Peptidase S8 and S53, subtilisin, kexin, sedolisin 2.2E-113
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021543.1 1 873-892
K04F10.4d 1 873-892
Sequence
Target Sequence 
>C. ELEGANS:191212_S_AT
agacagtcccagagcagcacatttgcacatatgaatacagacttgctaatccaaatcctc
ggccaattgtgggtcgtttccaacttaatttcactttagatgtgaatggatgcgagtcag
gaacccctgttttatatttggaacacgttcaagtgcatgctactgttcgatatctgaaac
gtggcgatctgaagcttacgctcttctcaccatccggcacccgatcggttcttcttccac
cacgaccacaagatttcaatgctaacggattccacaagtggcctttcctttcagtccaac
aatggggagaagatccacgtggaacatggcttctcatggtggaatcagtcaccactaatc
cagctgctactggtacgttccacgattggactcttcttctttatggaactgctgatccag
ctcaatcaggtgatcctgtctactcggctactcctgcaacatcccaaggagttctctcac
g

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGACAGTCCCAGAGCAGCACATTTG 278 71 1481 Antisense
CTCGGCCAATTGTGGGTCGTTTCCA 22 3 1538 Antisense
GAATGGATGCGAGTCAGGAACCCCT 211 331 1584 Antisense
CGATCTGAAGCTTACGCTCTTCTCA 144 249 1665 Antisense
TTCCACCACGACCACAAGATTTCAA 560 699 1715 Antisense
GATTCCACAAGTGGCCTTTCCTTTC 464 425 1748 Antisense
TCAGTCACCACTAATCCAGCTGCTA 479 623 1825 Antisense
CCAGCTGCTACTGGTACGTTCCACG 312 273 1840 Antisense
CACGATTGGACTCTTCTTCTTTATG 601 197 1861 Antisense
ATCAGGTGATCCTGTCTACTCGGCT 458 27 1905 Antisense
CAACATCCCAAGGAGTTCTCTCACG 397 189 1937 Antisense
Sequence Source Affymetrix Proprietary Database