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[191208_s_at] protein kinase

Description F55G1.8 /REP_DB=WormBase Gene ID /WP=CE07285 /GEN=plk-2 /TR=Q20845 /GB=AAB00642.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 4grp_high_8_8 > 31      6grp_total > 97      4grp_total > 24      6grp_cgc > 69      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 187
1 2 3 4 range
A 131.2
(-3)
A 		157.4
(-1.6)
P 		221
(1.8)
P 		242.5
(3)
P 		111
B 118.4
(-3)
P 		172.9
(-1.3)
P 		246.7
(1.1)
P 		305.5
(3)
P 		187
C 139.7
(-3)
P 		191.6
(-0.9)
P 		239.1
(1.1)
P 		285.8
(3)
P 		146
avr 129.8
(-3) 		174
(-1.2) 		235.6
(1.3) 		277.9
(3) 		148

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2966
1 2 3 4 5 6 range
A 87.6
(-3)
A 		127.8
(-2.9)
P 		201.4
(-2.7)
P 		669.1
(-1.7)
P 		2032.1
(1.4)
P 		2757.1
(3)
P 		2670
B 63
(-3)
A 		118.6
(-2.9)
P 		247.3
(-2.6)
P 		674
(-1.7)
P 		1840.3
(0.9)
P 		2817.2
(3)
P 		2754
C 53
(-3)
A 		100
(-2.9)
P 		280.8
(-2.5)
P 		785.5
(-1.5)
P 		1524.7
(-0)
P 		3018.6
(3)
P 		2966
avr 67.9
(-3) 		115.5
(-2.9) 		243.2
(-2.6) 		709.5
(-1.6) 		1799
(0.7) 		2864.3
(3) 		2796
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 49
1 2 3 4 range
A 47.8
(0.5)
A 		34.2
(-3)
P 		57.8
(3)
P 		35.5
(-2.7)
P 		24
B 37.8
(-3)
P 		42.7
(-1.9)
P 		57.2
(1.3)
P 		65.1
(3)
P 		27
C 46.2
(-3)
P 		55.1
(-1.6)
P 		83.3
(3)
P 		48.2
(-2.7)
P 		37
avr 43.9
(-3) 		44
(-3) 		66.1
(3) 		49.6
(-1.5) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1034
1 2 3 4 5 6 range
A 16.2
(-3)
A 		45.5
(-2.7)
P 		43.9
(-2.7)
P 		169.7
(-1.6)
P 		613.2
(2.5)
P 		671.3
(3)
P 		655
B 21.9
(-3)
A 		39.5
(-2.9)
P 		60
(-2.8)
P 		178.5
(-2.1)
P 		747.7
(1.2)
P 		1050.5
(3)
P 		1029
C 19.7
(-3)
A 		30.5
(-2.9)
P 		71.5
(-2.7)
P 		158
(-2.2)
P 		493.4
(-0.1)
P 		1010.5
(3)
P 		991
avr 19.3
(-3) 		38.5
(-2.9) 		58.5
(-2.7) 		168.7
(-2) 		618.1
(1) 		910.8
(3) 		892
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191208_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9141
Sequence Type Exemplar sequence
Representative Public ID F55G1.8 NCBI
Target Description F55G1.8 /REP_DB=WormBase Gene ID /WP=CE07285 /GEN=plk-2 /TR=Q20845 /GB=AAB00642.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068795(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068795 NCBI Caenorhabditis elegans POLO Kinase family member (plk-3) (plk-3) mRNA, complete cds. 11/11 None
SNAP00000005166 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7471047:7473193:-1 10/11 None
GENEFINDER00000005189 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7471047:7473193:-1 11/11 None
F55G1.8 ENSEMBL cdna:known chromosome:CEL140:IV:7470857:7473324:-1 gene:F55G1.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7471052-7473199(-) 100.0 100.0
Public Domain and Genome References
Gene Title protein kinase
Gene Symbol plk-3
Ensembl F55G1.8
Entrez Gene 266900 Entrez gene
SwissProt Q20845 EMBL-EBI
WormBase CE07285 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7049 cell cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB00642 Polo kinase protein 3 [Caenorhabditis elegans] ref|NP_501196.1| POLO Kinase family member (plk-3) [Caenorhabditis elegans] gb|AAC14425.1| polo-like kinase [Caenorhabditis elegans] sp|Q20845|PLK3_CAEEL Serine/threonine-protein kinase plk-3 (Polo-like kinase 3) 0.0
blast AAF36014 Polo kinase protein 2 [Caenorhabditis elegans] ref|NP_491036.1| POLO Kinase family member (plk-2) [Caenorhabditis elegans] gb|AAF28314.1| polo-like kinase 2 [Caenorhabditis elegans] sp|Q9N2L7|PLK2_CAEEL Serine/threonine-protein kinase plk-2 (Polo-like kinase-2) 1.0E-163
blast CAE60501 Hypothetical protein CBG04120 [Caenorhabditis briggsae] 1.0E-159
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-14
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-14
hanks 2.2.5 CaMK Group; CaMK II KIN1/SNF1/Nim1; SNF1 1.0E-71
Protein Domains
Database ID Description E-Value
Pfam IPR000959 EMBL-EBI POLO box duplicated region 1.7E-6
Pfam IPR000959 EMBL-EBI POLO box duplicated region 9.1E-20
Pfam IPR000719 EMBL-EBI Protein kinase 9.7E-84
Pfam IPR000959 EMBL-EBI POLO box duplicated region 9.1E-20
Sequence
Target Sequence 
>C. ELEGANS:191208_S_AT
aaaattcgtctggcatgctcttcaatgataacacacaaatgaaagtgaattcagccggaa
atcaactaactttcgttgacgagaataataccgaacaattttacaccatgaacgatcaag
taccgatgaacttgcagacgaagattaatatattttcaaatgtacaatcttacatgaaca
cccatttggaagctgacacccatttggaagctgaggatcaatgtgtcaataaagtgccac
cattacgcaactttgctcgacttccaacaatacaaaattggtttgaaacaaagtcggccg
ttatattccatctttcaaacgggacagttcagattaactttcttgatcatgtcaagatgg
tgctgtgtccgctgttgaagtctgtcacattcattgaggaaaacaaacgtgtttcaactt
tcacgttcgcaaacatattgacaaacagctgcccaaagaagtatctcagtcgtatcgaat
atgcccaagccaaaattaaattgcttcgtcctacaaacaaccaggagcatgtcgtctatg
tggactctccatgccgtcctacgacttcgaacacagctcacggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAATTCGTCTGGCATGCTCTTCAA 89 135 1244 Antisense
ACACCCATTTGGAAGCTGACACCCA 602 109 1421 Antisense
AAAGTGCCACCATTACGCAACTTTG 23 123 1474 Antisense
AACTTTGCTCGACTTCCAACAATAC 455 137 1492 Antisense
AAAGTCGGCCGTTATATTCCATCTT 124 123 1533 Antisense
TGTCAAGATGGTGCTGTGTCCGCTG 346 559 1593 Antisense
TGTCCGCTGTTGAAGTCTGTCACAT 346 557 1609 Antisense
CAGTCGTATCGAATATGCCCAAGCC 485 207 1710 Antisense
ATTGCTTCGTCCTACAAACAACCAG 582 5 1743 Antisense
GAGCATGTCGTCTATGTGGACTCTC 637 387 1768 Antisense
TACGACTTCGAACACAGCTCACGGT 277 647 1803 Antisense
Sequence Source Affymetrix Proprietary Database