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[191198_at] serine protease inhibitor

Description F35B12.4 /REP_DB=WormBase Gene ID /WP=CE05803 /TR=Q20008 /GB=CAA98461.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=serine protease inhibitor
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 86      4grp_highest_8_8 > 62      4grp_high_8_8 > 18      6grp_highest_nonorm > 82      6grp_total > 77      4grp_total > 29      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 468
1 2 3 4 range
A 1030
(3)
P 		826.2
(0.4)
P 		564
(-3)
P 		1019.3
(2.9)
P 		466
B 991.6
(3)
P 		739.2
(-1.4)
P 		645.9
(-3)
P 		865.7
(0.8)
P 		346
C 994
(3)
P 		850.4
(1)
P 		561.8
(-3)
P 		983.3
(2.9)
P 		432
avr 1005.2
(3) 		805.3
(0.1) 		590.6
(-3) 		956.1
(2.3) 		415

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 277
1 2 3 4 5 6 range
A 279.4
(-3)
P 		364.2
(-1.1)
P 		319
(-2.1)
P 		392.5
(-0.5)
P 		366.6
(-1.1)
P 		552.6
(3)
P 		273
B 276
(-3)
P 		357.6
(-0.6)
P 		401.6
(0.6)
P 		284.5
(-2.8)
P 		382.6
(0.1)
P 		484.1
(3)
P 		208
C 295.2
(-3)
P 		363.7
(-1)
P 		361.4
(-1)
P 		303.2
(-2.8)
P 		394.4
(-0.1)
P 		498.1
(3)
P 		203
avr 283.5
(-3) 		361.8
(-0.9) 		360.7
(-1) 		326.7
(-1.9) 		381.2
(-0.4) 		511.6
(3) 		228
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 228
1 2 3 4 range
A 375
(3)
P 		179.3
(-2.2)
P 		147.4
(-3)
P 		149.2
(-3)
P 		228
B 316.2
(3)
P 		182.6
(-1.8)
P 		149.7
(-3)
P 		184.5
(-1.7)
P 		167
C 329.1
(3)
P 		244.5
(-0.1)
P 		195.8
(-1.9)
P 		165.8
(-3)
P 		163
avr 340.1
(3) 		202.1
(-1.7) 		164.3
(-3) 		166.5
(-2.9) 		176

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 129
1 2 3 4 5 6 range
A 51.5
(-3)
P 		129.5
(2.6)
P 		69.5
(-1.7)
P 		99.5
(0.5)
P 		110.6
(1.3)
P 		134.5
(3)
P 		83
B 96
(-1.8)
P 		119
(-0.5)
P 		97.5
(-1.7)
P 		75.4
(-3)
P 		155.4
(1.6)
P 		180.5
(3)
P 		105
C 109.4
(-0.3)
P 		111
(-0.2)
P 		92
(-1.2)
P 		61
(-3)
P 		127.6
(0.8)
P 		166.8
(3)
P 		106
avr 85.6
(-2.5) 		119.8
(0) 		86.3
(-2.4) 		78.6
(-3) 		131.2
(0.8) 		160.6
(3) 		82
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191198_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13920
Sequence Type Exemplar sequence
Representative Public ID F35B12.4 NCBI
Target Description F35B12.4 /REP_DB=WormBase Gene ID /WP=CE05803 /TR=Q20008 /GB=CAA98461.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=serine protease inhibitor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073542(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073542 NCBI Caenorhabditis elegans F35B12.4 (F35B12.4) mRNA, complete cds. 11/11 None
SNAP00000002152 ENSEMBL cdna:SNAP chromosome:CEL140:V:11611828:11612657:-1 11/11 None
GENEFINDER00000002162 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11611828:11612657:-1 11/11 None
F35B12.4 ENSEMBL cdna:known chromosome:CEL140:V:11611828:11612717:-1 gene:F35B12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11611827-11612717(-) 100.0 100.0
Public Domain and Genome References
Gene Title serine protease inhibitor
Ensembl F35B12.4
Entrez Gene 185259 Entrez gene
SwissProt Q20008 EMBL-EBI
WormBase CE05803 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
4867 serine-type endopeptidase inhibitor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98461 Hypothetical protein F35B12.4 [Caenorhabditis elegans] ref|NP_505943.1| F35B12.4 [Caenorhabditis elegans] 1.0E-130
blast CAA98461 Hypothetical protein F35B12.4 [Caenorhabditis elegans] ref|NP_505943.1| F35B12.4 [Caenorhabditis elegans] 1.0E-118
blast CAE75329 Hypothetical protein CBG23307 [Caenorhabditis briggsae] 1.0E-103
blast CAE75327 Hypothetical protein CBG23305 [Caenorhabditis briggsae] 2.0E-37
Protein Domains
Database ID Description E-Value
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 1.7E-24
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 1.2E-10
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 1.7E-24
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 1.2E-10
Sequence
Target Sequence 
>C. ELEGANS:191198_AT
ttagattggctgtgattctctccctattgtctttcactgcggctcaatcgacttctgttg
actgttttgctgcacgggactctggaaatacttgcgctgagaacagttcttctagaatgt
tctattatcttccaagacttggaacgtgtcaaccattcatgtatcagggatgtggtggta
atagtaaccggttcagtagtgcacaagagtgtagatcagcttgtaccaatgctcaagctc
aaagagactcggagtcaagtgatgaaaccgttcaaatgaagtctgcttgctctgctacct
atgacactgaccacttgacaccagtgagatgctcagcaggagctaatacttgcccacagg
gtcataactgtattcaatcattctgttgtccaacatccaactatctttgcaacttgacat
atgactctggaaagtttgctgtcggaggagagaaaagtgacaaatacttctgggttccaa
aatacacaacttgcatgcgtttctcattct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTAGATTGGCTGTGATTCTCTCCCT 619 681 80 Antisense
GCTCAATCGACTTCTGTTGACTGTT 349 301 121 Antisense
TTGACTGTTTTGCTGCACGGGACTC 543 703 137 Antisense
GGGACTCTGGAAATACTTGCGCTGA 437 495 155 Antisense
GGAACGTGTCAACCATTCATGTATC 68 531 220 Antisense
GTAACCGGTTCAGTAGTGCACAAGA 546 461 263 Antisense
AAATGAAGTCTGCTTGCTCTGCTAC 693 119 353 Antisense
CTACCTATGACACTGACCACTTGAC 319 225 374 Antisense
GCAGGAGCTAATACTTGCCCACAGG 414 309 415 Antisense
TGTCCAACATCCAACTATCTTTGCA 543 557 466 Antisense
ACAACTTGCATGCGTTTCTCATTCT 648 111 565 Antisense
Sequence Source Affymetrix Proprietary Database