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[191190_at] G-protein coupled receptor

Description F53B7.2 /REP_DB=WormBase Gene ID /WP=CE05921 /TR=Q20715 /GB=CAA96651.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster 6grp_highest_10_10 > 43      6grp_high1_10_10 > 24      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 90      6grp_total > 02      4grp_total > 13      6grp_highest_sub > 02      6grp_high1_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 325
1 2 3 4 range
A 228.3
(-0.3)
P 		87.9
(-3)
P 		86
(-3)
P 		408.1
(3)
P 		322
B 238.1
(1.4)
P 		103.1
(-2.4)
P 		83
(-3)
P 		296
(3)
P 		213
C 258.6
(1.2)
P 		115.3
(-2.4)
P 		90.9
(-3)
P 		332.3
(3)
P 		241
avr 241.7
(0.6) 		102.1
(-2.6) 		86.6
(-3) 		345.5
(3) 		259

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 190
1 2 3 4 5 6 range
A 146.4
(2)
P 		158.5
(2.6)
P 		167.2
(3)
P 		116.6
(0.6)
P 		146.9
(2)
P 		41.2
(-3)
P 		126
B 161.9
(-0)
P 		230.7
(3)
P 		101.4
(-2.7)
P 		105
(-2.6)
P 		111.8
(-2.3)
P 		95.2
(-3)
P 		136
C 119.4
(-1)
P 		213.2
(3)
P 		73.2
(-3)
P 		87.2
(-2.4)
P 		87.7
(-2.4)
P 		88.4
(-2.3)
P 		140
avr 142.6
(0.2) 		200.8
(3) 		113.9
(-1.1) 		102.9
(-1.7) 		115.5
(-1.1) 		74.9
(-3) 		126
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 67
1 2 3 4 range
A 83.1
(3)
P 		19.1
(-3)
P 		22.5
(-2.7)
P 		59.7
(0.8)
P 		64
B 75.9
(3)
P 		25.5
(-2.3)
P 		19.2
(-3)
P 		63.1
(1.6)
P 		57
C 85.6
(3)
P 		33.1
(-2.8)
P 		31.7
(-3)
P 		56
(-0.3)
P 		54
avr 81.5
(3) 		25.9
(-2.9) 		24.5
(-3) 		59.6
(0.7) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 67
1 2 3 4 5 6 range
A 27
(-0.8)
P 		56.4
(3)
P 		36.4
(0.4)
P 		29.6
(-0.5)
P 		44.3
(1.4)
P 		10
(-3)
P 		46
B 56.3
(0.6)
P 		76.8
(3)
P 		24.6
(-3)
P 		27.8
(-2.6)
P 		45.4
(-0.6)
P 		35.5
(-1.7)
P 		52
C 44.2
(0.4)
P 		65
(3)
P 		18.6
(-2.9)
P 		17.5
(-3)
P 		28.4
(-1.6)
P 		29.6
(-1.5)
P 		48
avr 42.5
(-0.4) 		66.1
(3) 		26.5
(-2.8) 		25
(-3) 		39.4
(-0.9) 		25
(-3) 		41
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191190_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13409
Sequence Type Exemplar sequence
Representative Public ID F53B7.2 NCBI
Target Description F53B7.2 /REP_DB=WormBase Gene ID /WP=CE05921 /TR=Q20715 /GB=CAA96651.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073401(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073401 NCBI Caenorhabditis elegans F53B7.2 (F53B7.2) mRNA, complete cds. 11/11 None
SNAP00000012644 ENSEMBL cdna:SNAP chromosome:CEL140:V:10994686:10997404:1 9/11 None
GENEFINDER00000012650 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10994686:10997404:1 11/11 None
F53B7.2 ENSEMBL cdna:known chromosome:CEL140:V:10994524:10997404:1 gene:F53B7.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10994523-10997404(+) 98.02 100.0
Public Domain and Genome References
Gene Title G-protein coupled receptor
Ensembl F53B7.2
Entrez Gene 186155 Entrez gene
SwissProt Q20715 EMBL-EBI
WormBase CE05921 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
1584 rhodopsin-like receptor activity inferred from electronic annotation QuickGO AmiGO
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA96651 Hypothetical protein F53B7.2 [Caenorhabditis elegans] ref|NP_505802.1| F53B7.2 [Caenorhabditis elegans] 0.0
blast CAE75139 Hypothetical protein CBG23068 [Caenorhabditis briggsae] 0.0
blast CAE63966 Hypothetical protein CBG08555 [Caenorhabditis briggsae] 8.0E-26
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.9E-6
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505802.1 6 85-107,119-141,161-183,204-226,261-283,350-372
F53B7.2 6 85-107,119-141,161-183,204-226,261-283,350-372
SNAP00000012644 6 31-53,65-87,107-129,150-172,207-229,269-291
GENEFINDER00000012650 6 31-53,65-87,107-129,150-172,207-229,305-327
Sequence
Target Sequence 
>C. ELEGANS:191190_AT
tgctgtaattgtcttgcattctgcaacaacatctacgaaaacgtgcaacatgtaaaaaaa
catgcatcggattctcaatccctgaatcaaacttcatttgttccagagatcgaagaacaa
tcacaagattatgatggtgaatggagcatttttaatgaatggaactttgacctttctgtt
gaaatatcaaatcttctaatcagtttgaattcatccagttctatgtttgtatatctgatt
ttttcatcaaagtacagatcaattatcaaacactggctgggattagagaagagaaaaaga
acaaatggagttgccttaacaacagtgatggccgctcagaaagcattggagctttcgatt
cttccagatgaagttgaagcccgtcgtcatagaaaagaaaaatcacattttgtaaaaaat
aagaaacaaatgaacaaatcagctcaattatttctcacaacatcagaaattgatcttcga
aaagcaagaacgattcgaaaagaacaagaagacgaagaagaagaagaggaagagattcga
gaaattcaaagcgatgagccaagtgcagccaatgatcaaaaccgttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTGTAATTGTCTTGCATTCTGCA 557 581 1102 Antisense
TGCATTCTGCAACAACATCTACGAA 328 579 1116 Antisense
CATGCATCGGATTCTCAATCCCTGA 261 211 1162 Antisense
GGAACTTTGACCTTTCTGTTGAAAT 532 529 1262 Antisense
GAATTCATCCAGTTCTATGTTTGTA 324 325 1308 Antisense
TAACAACAGTGATGGCCGCTCAGAA 427 639 1418 Antisense
GGAGCTTTCGATTCTTCCAGATGAA 451 521 1449 Antisense
TGAAGTTGAAGCCCGTCGTCATAGA 261 575 1470 Antisense
AGCTCAATTATTTCTCACAACATCA 506 79 1542 Antisense
AAGCGATGAGCCAAGTGCAGCCAAT 198 149 1650 Antisense
GTGCAGCCAATGATCAAAACCGTTC 163 481 1664 Antisense
Sequence Source Affymetrix Proprietary Database