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[191185_at] 7TM chemoreceptor, srj family

Description F31F4.16 /REP_DB=WormBase Gene ID /WP=CE09836 /GEN=srj-5 /TR=O17124 /GB=AAG24099.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster 6grp_total > 99      4grp_total > 03      6grp_cgc > 19      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 105
1 2 3 4 range
A 4.8
(-2.9)
A 		4.5
(-3)
A 		6.7
(-2.6)
A 		40.4
(3)
A 		36
B 28.3
(-0.8)
A 		6.1
(-2.6)
A 		1.7
(-3)
A 		74.6
(3)
A 		73
C 10.7
(-3)
A 		11.3
(-3)
A 		13.7
(-2.8)
A 		106.7
(3)
A 		96
avr 14.6
(-2.3) 		7.3
(-3) 		7.4
(-3) 		73.9
(3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 6.5
(0.3)
A 		4.3
(-3)
A 		4.9
(-2.1)
A 		6.5
(0.3)
A 		8.3
(3)
A 		5
(-2)
A 		4
B 5.5
(-2.3)
A 		5.1
(-2.4)
A 		2.6
(-3)
A 		4.3
(-2.6)
A 		25.6
(2.3)
A 		28.7
(3)
A 		26
C 4.1
(-2.7)
A 		24.2
(3)
A 		3.6
(-2.8)
A 		3
(-3)
A 		3
(-3)
A 		3.7
(-2.8)
A 		21
avr 5.4
(-1.8) 		11.2
(2.1) 		3.7
(-3) 		4.6
(-2.4) 		12.3
(2.9) 		12.5
(3) 		9
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 1.8
(-2)
A 		1
(-3)
A 		1.8
(-2)
A 		5.9
(3)
A 		5
B 9
(0.3)
A 		1.5
(-2.6)
A 		0.4
(-3)
A 		15.9
(3)
A 		16
C 3.6
(-2.8)
A 		3.2
(-3)
A 		4.8
(-2.4)
A 		18
(3)
A 		15
avr 4.8
(-1.5) 		1.9
(-3) 		2.3
(-2.8) 		13.3
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 1.2
(-2.6)
A 		1.5
(-1.3)
A 		1.1
(-3)
A 		1.6
(-0.9)
A 		2.5
(3)
A 		1.2
(-2.6)
A 		1
B 1.9
(-2.2)
A 		1.7
(-2.3)
A 		0.6
(-3)
A 		1.1
(-2.7)
A 		10.4
(2.8)
A 		10.7
(3)
A 		10
C 1.5
(-2.2)
A 		7.4
(3)
A 		0.9
(-2.7)
A 		0.6
(-3)
A 		1
(-2.6)
A 		1.2
(-2.5)
A 		7
avr 1.5
(-2) 		3.5
(1.2) 		0.9
(-3) 		1.1
(-2.7) 		4.6
(3) 		4.4
(2.7) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191185_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15346
Sequence Type Exemplar sequence
Representative Public ID F31F4.16 NCBI
Target Description F31F4.16 /REP_DB=WormBase Gene ID /WP=CE09836 /GEN=srj-5 /TR=O17124 /GB=AAG24099.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070878(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070878 NCBI Caenorhabditis elegans Serpentine Receptor, class J family member (srj-5) (srj-5) mRNA, complete cds. 11/11 None
SNAP00000012360 ENSEMBL cdna:SNAP chromosome:CEL140:V:651044:652839:-1 11/11 None
GENEFINDER00000012378 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:650100:655615:-1 11/11 None
F31F4.16 ENSEMBL cdna:known chromosome:CEL140:V:651666:652869:-1 gene:F31F4.16 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:651665-652869(-) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, srj family
Gene Symbol srj-5
Ensembl F31F4.16
Entrez Gene 191932 Entrez gene
SwissProt O17124 EMBL-EBI
WormBase CE09836 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503279 Serpentine Receptor, class J family member (srj-5) [Caenorhabditis elegans] gb|AAG24099.1| Serpentine receptor, class j protein 5 [Caenorhabditis elegans] 0.0
blast NP_503280 Serpentine Receptor, class J family member (srj-6) [Caenorhabditis elegans] gb|AAG24098.3| Serpentine receptor, class j protein 6 [Caenorhabditis elegans] pir||T32305 hypothetical protein F31F4.8 - Caenorhabditis elegans 1.0E-172
blast NP_503280 Serpentine Receptor, class J family member (srj-6) [Caenorhabditis elegans] gb|AAG24098.3| Serpentine receptor, class j protein 6 [Caenorhabditis elegans] pir||T32305 hypothetical protein F31F4.8 - Caenorhabditis elegans 1.0E-167
blast CAE71085 Hypothetical protein CBG17935 [Caenorhabditis briggsae] 1.0E-148
blast CAE71085 Hypothetical protein CBG17935 [Caenorhabditis briggsae] 1.0E-142
blast NP_503279 Serpentine Receptor, class J family member (srj-5) [Caenorhabditis elegans] gb|AAG24099.1| Serpentine receptor, class j protein 5 [Caenorhabditis elegans] 1.0E-122
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.2E-51
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.2E-4
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.2E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503279.1 7 10-32,45-67,96-115,128-147,196-218,244-266,279-301
F31F4.16 7 10-32,45-67,96-115,128-147,196-218,244-266,279-301
SNAP00000012360 7 4-21,34-56,86-105,118-137,186-208,234-256,269-291
GENEFINDER00000012378 12 20-42,46-68,89-108,147-169,190-212,292-314,340-362,382-401,449-471,498-520,532-554,574-596
Sequence
Target Sequence 
>C. ELEGANS:191185_AT
cttgctatgattgttcttactgctggtcacggagcatcgtggacatggctctgtgagggc
tgcttgtcaccaaatgaagagataagagacatgatacggcctgcattcttagaaattcac
cacgtcaactcggataatatttctctgttgacagggcagtatagaaacgctagtgacttt
gttgtctacaaaagtttgtttggtatcatgtcacttacattattttcgagctactgtatg
tgtgtttatattattcttggctataaaataatgaagaaaatgaatgaaaacacaaatatg
agctcgattagcgcaacactgaatcggcaactctttaaagcgctggttgctcagacgtgc
atcccaatgttcgccagcttcctaccaactgtcatcgcctggtacgcaccgatgtttttg
atcaatgttacttggtggaacaactacatctgtaatgttgcattggctgcctttcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTGCTATGATTGTTCTTACTGCTG 386 217 403 Antisense
CTTACTGCTGGTCACGGAGCATCGT 427 221 418 Antisense
TGGACATGGCTCTGTGAGGGCTGCT 347 547 442 Antisense
TGAGGGCTGCTTGTCACCAAATGAA 585 567 456 Antisense
AGACATGATACGGCCTGCATTCTTA 303 71 489 Antisense
GAAATTCACCACGTCAACTCGGATA 403 361 514 Antisense
GTCACTTACATTATTTTCGAGCTAC 199 465 612 Antisense
ATGAGCTCGATTAGCGCAACACTGA 459 43 700 Antisense
GCAACACTGAATCGGCAACTCTTTA 50 319 715 Antisense
TAAAGCGCTGGTTGCTCAGACGTGC 238 639 738 Antisense
GTAATGTTGCATTGGCTGCCTTTCC 8 463 854 Antisense
Sequence Source Affymetrix Proprietary Database