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[191181_at] G-protein coupled receptor

Description ZK455.3 /REP_DB=WormBase Gene ID /WP=CE03814 /TR=Q23497 /GB=CAA91489.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster 6grp_total > 44      4grp_total > 06      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 80.1
(0.5)
A 		56.3
(-3)
A 		75.2
(-0.2)
A 		96.9
(3)
A 		41
B 86.1
(3)
A 		59.7
(-3)
A 		69.5
(-0.8)
A 		60.5
(-2.8)
A 		26
C 54.4
(-0.4)
A 		51.6
(-0.7)
A 		31.4
(-3)
A 		85.2
(3)
A 		54
avr 73.5
(1.2) 		55.9
(-3) 		58.7
(-2.3) 		80.9
(3) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 64
1 2 3 4 5 6 range
A 10.1
(-3)
A 		53.2
(1.6)
A 		66.4
(3)
A 		38.1
(-0)
A 		38.7
(0)
A 		33.6
(-0.5)
A 		56
B 62.1
(3)
A 		54.1
(1.9)
A 		41.6
(0.2)
A 		17.6
(-3)
A 		29
(-1.5)
A 		49.4
(1.3)
A 		45
C 38.4
(-1.3)
A 		65.6
(2)
A 		73.9
(3)
A 		23.9
(-3)
A 		43.3
(-0.7)
A 		42.1
(-0.8)
A 		50
avr 36.9
(-1.2) 		57.6
(2.5) 		60.6
(3) 		26.5
(-3) 		37
(-1.2) 		41.7
(-0.3) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 29.2
(3)
A 		12.2
(-3)
A 		19.7
(-0.4)
A 		14.2
(-2.3)
A 		17
B 27.4
(3)
A 		14.7
(-2.3)
A 		16.1
(-1.7)
A 		12.9
(-3)
A 		15
C 18
(3)
A 		14.8
(0.3)
A 		11
(-3)
A 		14.4
(-0.1)
A 		7
avr 24.9
(3) 		13.9
(-2.9) 		15.6
(-2) 		13.8
(-3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 1.9
(-3)
A 		18.9
(3)
A 		14.5
(1.4)
A 		9.7
(-0.2)
A 		11.7
(0.5)
A 		8.2
(-0.8)
A 		17
B 21.6
(3)
A 		18
(1.7)
A 		10.1
(-1.1)
A 		4.7
(-3)
A 		11.8
(-0.5)
A 		18.4
(1.9)
A 		17
C 14.2
(0.7)
A 		20
(3)
A 		18.8
(2.5)
A 		4.8
(-3)
A 		14
(0.6)
A 		14.1
(0.7)
A 		15
avr 12.6
(-0) 		19
(3) 		14.5
(0.9) 		6.4
(-3) 		12.5
(-0.1) 		13.6
(0.4) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191181_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16983
Sequence Type Exemplar sequence
Representative Public ID ZK455.3 NCBI
Target Description ZK455.3 /REP_DB=WormBase Gene ID /WP=CE03814 /TR=Q23497 /GB=CAA91489.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=G-protein coupled receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077495(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077495 NCBI Caenorhabditis elegans ZK455.3 (ZK455.3) mRNA, complete cds. 11/11 None
SNAP00000004975 ENSEMBL cdna:SNAP chromosome:CEL140:X:11329648:11331792:1 11/11 None
GENEFINDER00000004984 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:11329648:11331996:1 11/11 None
ZK455.3 ENSEMBL cdna:known chromosome:CEL140:X:11328515:11331996:1 gene:ZK455.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:11328514-11331996(+) 100.0 100.0
Public Domain and Genome References
Gene Title G-protein coupled receptor
Ensembl ZK455.3
Entrez Gene 191320 Entrez gene
SwissProt Q23497 EMBL-EBI
WormBase CE03814 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.8464.1.S1_AT similar to galanin receptor 2 cfa
DROSOPHILA_2:1629186_A_AT Allatostatin Receptor dm
DROSGENOME1:144333_AT Allatostatin Receptor dm
CHICKEN:GGAAFFX.1222.1.S1_AT similar to Galanin receptor type 2 (GAL2-R) (GALR2) gga
CHICKEN:GGAAFFX.1222.1.S1_S_AT similar to Galanin receptor type 2 (GAL2-R) (GALR2) gga
CHICKEN:GGAAFFX.1222.2.S1_AT similar to Galanin receptor type 2 (GAL2-R) (GALR2) gga
CHICKEN:GGAAFFX.1242.1.S1_AT similar to Galanin receptor type 2 (GAL2-R) (GALR2) gga
HG-U133_PLUS_2:211226_AT galanin receptor 2 hs
HG-U133A:211226_AT galanin receptor 2 hs
HG-FOCUS:211226_AT galanin receptor 2 hs
HG-U133A_2:211226_AT galanin receptor 2 hs
U133_X3P:G4165080_3P_AT galanin receptor 2 hs
HG-U95AV2:31905_AT galanin receptor 2 hs
MG-U74AV2:99430_AT galanin receptor 2 mm
MOUSE430_2:1422942_AT galanin receptor 2 mm
MOE430A:1422942_AT galanin receptor 2 mm
MOUSE430A_2:1422942_AT galanin receptor 2 mm
RN-U34:U94322_S_AT galanin receptor 2 rn
RG-U34A:U94322_S_AT galanin receptor 2 rn
RG-U34C:RC_AI071642_AT galanin receptor 2 rn
RAE230A:1368758_A_AT galanin receptor 2 rn
RAT230_2:1368758_A_AT galanin receptor 2 rn
RAE230B:1384667_X_AT galanin receptor 2 rn
RAT230_2:1384667_X_AT galanin receptor 2 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
1584 rhodopsin-like receptor activity inferred from electronic annotation QuickGO AmiGO
4966 galanin receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91489 Hypothetical protein ZK455.3 [Caenorhabditis elegans] ref|NP_509896.1| ZK455.3 [Caenorhabditis elegans] 0.0
blast CAE70656 Hypothetical protein CBG17363 [Caenorhabditis briggsae] 1.0E-170
blast CAE70656 Hypothetical protein CBG17363 [Caenorhabditis briggsae] 1.0E-169
blast CAB77263 G-protein coupled receptor [Lymnaea stagnalis] 4.0E-61
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 2.2E-62
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 2.2E-62
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509896.1 7 44-66,79-101,116-138,159-178,214-236,284-306,321-343
ZK455.3 7 44-66,79-101,116-138,159-178,214-236,284-306,321-343
SNAP00000004975 7 30-52,65-87,102-124,145-164,200-222,270-292,307-329
GENEFINDER00000004984 7 30-52,65-87,102-124,145-164,200-222,270-292,307-329
Sequence
Target Sequence 
>C. ELEGANS:191181_AT
gacatgccaaggccgggtaactctcaatctgttggtggtagaaatcttacaaatcgtgat
agtggttcaagtattcgtcggaggccggaagcaactgctgccaaaagaaaagttacccgc
cttgtactgtgtgttttgatcacctgggcactttgctggcttccactaaacgtgtgcttt
ttcatgtctggtctcgcctacccggaaccacttgttatatctcatggtgtcatcatggta
attgttcagattgctagtcaagttcttgcctacaccaattcgtgtcttaatccaattcta
tacgctttgatgtcgcagagtttccgggaaggattcattcgagtcatgaaaatgctaata
aataagctatcgcgtggaaggttctgcactaattaccgacgaagtgctcttcggacagag
ctcacacactataaccaaactccagcacatccagcaaacactgtggttcaagtgtcaaac
ggagaacgatcctcgcttctgaaagataactcgagctctgcgacgtctgtacaaccactt
cggacgtcaattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACATGCCAAGGCCGGGTAACTCTC 68 369 739 Antisense
GTGGTTCAAGTATTCGTCGGAGGCC 710 421 800 Antisense
TCGGAGGCCGGAAGCAACTGCTGCC 591 605 816 Antisense
TGTTTTGATCACCTGGGCACTTTGC 59 561 870 Antisense
CACTTTGCTGGCTTCCACTAAACGT 436 197 887 Antisense
AAACGTGTGCTTTTTCATGTCTGGT 502 127 906 Antisense
GCTAGTCAAGTTCTTGCCTACACCA 686 285 991 Antisense
GGACAGAGCTCACACACTATAACCA 588 517 1151 Antisense
CAAACGGAGAACGATCCTCGCTTCT 549 191 1214 Antisense
AAGATAACTCGAGCTCTGCGACGTC 398 153 1241 Antisense
GTACAACCACTTCGGACGTCAATTC 658 459 1267 Antisense
Sequence Source Affymetrix Proprietary Database