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[191177_at] potassium channel protein

Description F44A2.2 /REP_DB=WormBase Gene ID /WP=CE04569 /TR=Q20381 /GB=AAA83447.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=potassium channel protein
Cluster 6grp_high1_10_10 > 17      4grp_high_8_8 > 56      6grp_total > 07      4grp_total > 07      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 186.4
(3)
P 		124
(-3)
A 		131.6
(-2.3)
M 		146.1
(-0.9)
P 		62
B 146
(-0.6)
P 		125.5
(-3)
P 		176.8
(3)
P 		139.3
(-1.4)
P 		51
C 112.7
(-3)
P 		146.1
(3)
P 		132.8
(0.6)
P 		140.4
(2)
P 		33
avr 148.4
(3) 		131.9
(-3) 		147.1
(2.5) 		141.9
(0.6) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 172
1 2 3 4 5 6 range
A 202
(3)
P 		97.1
(-0.7)
P 		101.3
(-0.6)
P 		57.5
(-2.1)
P 		33.3
(-3)
A 		78
(-1.4)
P 		169
B 167
(3)
P 		111.6
(-0.5)
P 		122.7
(0.2)
P 		86
(-2.1)
P 		99
(-1.3)
P 		71
(-3)
P 		96
C 205.7
(3)
P 		112.4
(-0.7)
P 		105.5
(-1)
P 		107.8
(-0.9)
P 		55
(-3)
A 		85.2
(-1.8)
P 		151
avr 191.6
(3) 		107
(-0.9) 		109.8
(-0.8) 		83.8
(-2) 		62.4
(-3) 		78.1
(-2.3) 		129
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 67.9
(3)
P 		26.9
(-2.3)
A 		34.4
(-1.3)
M 		21.4
(-3)
P 		47
B 46.5
(3)
P 		31
(-2.5)
P 		41
(1)
P 		29.7
(-3)
P 		17
C 37.3
(0.6)
P 		42
(1.9)
P 		46.3
(3)
P 		23.7
(-3)
P 		23
avr 50.6
(3) 		33.3
(-1) 		40.6
(0.7) 		24.9
(-3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 37.2
(3)
P 		34.5
(2.4)
P 		22.1
(-0.3)
P 		14.6
(-2)
P 		10
(-3)
A 		19
(-1)
P 		27
B 58.1
(3)
P 		37.2
(-0.6)
P 		29.8
(-1.8)
P 		22.8
(-3)
P 		40.2
(-0)
P 		26.5
(-2.4)
P 		35
C 76.2
(3)
P 		34.3
(-1.3)
P 		26.8
(-2.1)
P 		21.7
(-2.6)
P 		17.8
(-3)
A 		28.5
(-1.9)
P 		58
avr 57.2
(3) 		35.3
(-0.5) 		26.2
(-2) 		19.7
(-3) 		22.7
(-2.5) 		24.7
(-2.2) 		38
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191177_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11492
Sequence Type Exemplar sequence
Representative Public ID F44A2.2 NCBI
Target Description F44A2.2 /REP_DB=WormBase Gene ID /WP=CE04569 /TR=Q20381 /GB=AAA83447.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=potassium channel protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073006(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073006 NCBI Caenorhabditis elegans F44A2.2 (F44A2.2) mRNA, complete cds. 11/11 None
SNAP00000038853 ENSEMBL cdna:SNAP chromosome:CEL140:V:9320897:9323465:1 11/11 None
F44A2.2 ENSEMBL cdna:known chromosome:CEL140:V:9320897:9323465:1 gene:F44A2.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000038859 7/11 Cross Hyb Matching Probes None
GENEFINDER00000038863 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9320896-9323465(+) 100.0 100.0
Public Domain and Genome References
Gene Title potassium channel protein
Ensembl F44A2.2
Entrez Gene 185719 Entrez gene
SwissProt Q20381 EMBL-EBI
WormBase CE35307 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1582552_AT potassium voltage-gated channel, Shab-related subfamily, member 2 cfa
CANINE_2:CFA.3787.1.S1_AT potassium voltage-gated channel, Shab-related subfamily, member 2 cfa
CANINE_2:CFA.3787.1.S1_S_AT potassium voltage-gated channel, Shab-related subfamily, member 2 cfa
HG-U95AV2:31312_AT potassium voltage-gated channel, Shab-related subfamily, member 2 hs
HG-U133A:208123_AT potassium voltage-gated channel, Shab-related subfamily, member 2 hs
HG-U133_PLUS_2:208123_AT potassium voltage-gated channel, Shab-related subfamily, member 2 hs
HG-FOCUS:208123_AT potassium voltage-gated channel, Shab-related subfamily, member 2 hs
HG-U133A_2:208123_AT potassium voltage-gated channel, Shab-related subfamily, member 2 hs
HG-U133A:208172_S_AT potassium voltage-gated channel, Shab-related subfamily, member 2 hs
HG-U133_PLUS_2:208172_S_AT potassium voltage-gated channel, Shab-related subfamily, member 2 hs
HG-U133A_2:208172_S_AT potassium voltage-gated channel, Shab-related subfamily, member 2 hs
U133_X3P:G4758621_3P_A_AT potassium voltage-gated channel, Shab-related subfamily, member 2 hs
U133_X3P:G13242171_3P_AT potassium voltage-gated channel, Shab-related subfamily, member 2 hs
HG-U133_PLUS_2:237007_AT Potassium voltage-gated channel, Shab-related subfamily, member 2 hs
HG-U133B:237007_AT Potassium voltage-gated channel, Shab-related subfamily, member 2 hs
HG-U95B:47817_AT Potassium voltage-gated channel, Shab-related subfamily, member 2 hs
HG-U95C:59864_AT Potassium voltage-gated channel, Shab-related subfamily, member 2 hs
HU35KSUBA:RC_AA017045_AT Potassium voltage-gated channel, Shab-related subfamily, member 2 hs
U133_X3P:HS.6702.0.A1_3P_AT Potassium voltage-gated channel, Shab-related subfamily, member 2 hs
RN-U34:M77482_AT potassium voltage gated channel, Shab-related subfamily, member 2 rn
RG-U34A:M77482_AT potassium voltage gated channel, Shab-related subfamily, member 2 rn
RAE230A:1369369_AT potassium voltage gated channel, Shab-related subfamily, member 2 rn
RAT230_2:1369369_AT potassium voltage gated channel, Shab-related subfamily, member 2 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6811 ion transport inferred from electronic annotation QuickGO AmiGO
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
8076 voltage-gated potassium channel complex inferred from electronic annotation QuickGO AmiGO
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5216 ion channel activity inferred from electronic annotation QuickGO AmiGO
5249 voltage-gated potassium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA83447 Hypothetical protein F44A2.2 [Caenorhabditis elegans] ref|NP_505407.2| F44A2.2 [Caenorhabditis elegans] 0.0
blast CAE72151 Hypothetical protein CBG19249 [Caenorhabditis briggsae] 0.0
blast CAE64863 Hypothetical protein CBG09661 [Caenorhabditis briggsae] 7.0E-77
blast CAE64863 Hypothetical protein CBG09661 [Caenorhabditis briggsae] 3.0E-57
Protein Domains
Database ID Description E-Value
Pfam IPR005821 EMBL-EBI Ion transport protein 9.7E-37
Pfam IPR003131 EMBL-EBI K+ channel tetramerisation 7.2E-39
Pfam IPR005821 EMBL-EBI Ion transport protein 3.3E-16
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505407.2 5 179-201,216-238,317-339,349-371,378-400
F44A2.2 5 179-201,216-238,317-339,349-371,378-400
SNAP00000038853 4 179-201,216-238,315-337,342-364
Sequence
Target Sequence 
>C. ELEGANS:191177_AT
ctcattatctccttcattggctggaattattgtgtatggtgtacttcacatttgaatacc
ttgcacgtctccttgtgaatccgaaaaaagcagaatttattaggagtccactgaacgtta
ttgacttgctgactgtgttgccgtttatgattgaagcgtttaatgaattgcaatggatga
aagagtttagaggtgcaatgcttgtggttcgagtaatgagacttgcccgtgtagcgcgta
tcttcaagctcgccaggtactcaactggtctccgtgcctttggagaaacaatgaaaaagt
ctgcggcagagctcagtatgcttggaatgtttttggtaacaggtgtcataacaatgacca
cagttggctatggtgatttggtgcctataactgctggaggaaaagtggttgctgctttgg
cctcagtgtgtggaatcattgtgctcgcattcccaatttcaatgataatcgacaaatttg
ctgaatcgacaggagggtggagcggtggagatggagacgaagaacaagggcacatgattg
tgcatcggactgtacacccattgaataatggagcaccggctgccca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCATTATCTCCTTCATTGGCTGGA 485 229 653 Antisense
ACATTTGAATACCTTGCACGTCTCC 494 103 700 Antisense
TTGCACGTCTCCTTGTGAATCCGAA 701 703 713 Antisense
TTGACTTGCTGACTGTGTTGCCGTT 178 703 773 Antisense
TGTAGCGCGTATCTTCAAGCTCGCC 614 559 882 Antisense
GCTCGCCAGGTACTCAACTGGTCTC 229 297 900 Antisense
GTCTGCGGCAGAGCTCAGTATGCTT 632 469 951 Antisense
GAAAAGTGGTTGCTGCTTTGGCCTC 334 353 1052 Antisense
GGAATCATTGTGCTCGCATTCCCAA 350 533 1084 Antisense
TGCATCGGACTGTACACCCATTGAA 615 577 1193 Antisense
TGAATAATGGAGCACCGGCTGCCCA 676 575 1214 Antisense
Sequence Source Affymetrix Proprietary Database