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[191174_s_at]

Description C27A7.5C /REP_DB=WormBase Gene ID /WP=CE20529 /TR=Q9U3P0 /GB=CAB02790.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Asparaginase ; Ank repeat
Cluster 6grp_highest_10_10 > 09      6grp_high1_10_10 > 90      4grp_high_8_8 > 49      6grp_highest_nonorm > 62      6grp_total > 10      4grp_total > 62      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 385
1 2 3 4 range
A 567.3
(3)
P 		444.9
(0.9)
P 		465.2
(1.3)
P 		215.2
(-3)
A 		352
B 600.2
(3)
P 		374.6
(-0.9)
P 		351.6
(-1.3)
P 		253.1
(-3)
P 		347
C 540.3
(3)
P 		442.3
(0.6)
P 		486.9
(1.7)
P 		297.9
(-3)
P 		242
avr 569.3
(3) 		420.6
(0.2) 		434.6
(0.4) 		255.4
(-3) 		314

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 139
1 2 3 4 5 6 range
A 433.9
(-3)
P 		474.8
(-0.5)
P 		455.1
(-1.7)
P 		508.3
(1.5)
P 		533.2
(3)
P 		472.8
(-0.6)
P 		99
B 447
(-1.7)
P 		500.3
(3)
P 		469.3
(0.3)
P 		432.3
(-3)
P 		478.7
(1.1)
P 		453.9
(-1.1)
P 		68
C 454.7
(-1)
P 		547.1
(3)
P 		478.9
(0.1)
P 		487.6
(0.4)
P 		458.9
(-0.8)
P 		407.7
(-3)
P 		139
avr 445.2
(-3) 		507.4
(3) 		467.8
(-0.8) 		476.1
(0) 		490.3
(1.4) 		444.8
(-3) 		63
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 175
1 2 3 4 range
A 206.5
(3)
P 		96.6
(-0.8)
P 		121.6
(0.1)
P 		31.5
(-3)
A 		175
B 191.4
(3)
P 		92.5
(-1.3)
P 		81.5
(-1.8)
P 		53.9
(-3)
P 		138
C 178.9
(3)
P 		127.2
(0.6)
P 		169.7
(2.6)
P 		50.2
(-3)
P 		129
avr 192.3
(3) 		105.4
(-0.5) 		124.3
(0.2) 		45.2
(-3) 		147

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 115
1 2 3 4 5 6 range
A 80
(-3)
P 		168.9
(3)
P 		99.1
(-1.7)
P 		128.9
(0.3)
P 		160.9
(2.5)
P 		115.1
(-0.6)
P 		89
B 155.5
(0.1)
P 		166.5
(0.9)
P 		114
(-3)
P 		114.5
(-3)
P 		194.5
(3)
P 		169.2
(1.1)
P 		81
C 168.6
(3)
P 		166.9
(2.9)
P 		121.9
(-1)
P 		98
(-3)
P 		148.5
(1.3)
P 		136.5
(0.3)
P 		71
avr 134.7
(-0.5) 		167.4
(2.9) 		111.7
(-3) 		113.8
(-2.8) 		168
(3) 		140.3
(0) 		56
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191174_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12939
Sequence Type Exemplar sequence
Representative Public ID C27A7.5C NCBI
Target Description C27A7.5C /REP_DB=WormBase Gene ID /WP=CE20529 /TR=Q9U3P0 /GB=CAB02790.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Asparaginase ; Ank repeat
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073648(11), NM_073647(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073648 NCBI Caenorhabditis elegans C27A7.5a (asparaginase) mRNA, complete cds. 11/11 A 
NM_073647 NCBI Caenorhabditis elegans C27A7.5c (asparaginase) mRNA, complete cds. 11/11 A 
SNAP00000042926 ENSEMBL cdna:SNAP chromosome:CEL140:V:12157615:12160932:-1 11/11 None
GENEFINDER00000042934 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:12157615:12160932:-1 11/11 None
C27A7.5a ENSEMBL cdna:known chromosome:CEL140:V:12157465:12164887:-1 gene:C27A7.5 11/11 A 
C27A7.5c ENSEMBL cdna:known chromosome:CEL140:V:12157615:12164886:-1 gene:C27A7.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179667 3 2 NM_073647 174881_s_at (11), 191174_s_at (11)
NM_073648 174881_s_at (11), 191174_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:12157614-12164885(-) 100.0 100.0
Public Domain and Genome References
Ensembl C27A7.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB02791 Hypothetical protein C27A7.5a [Caenorhabditis elegans] ref|NP_506049.1| C27A7.5a [Caenorhabditis elegans] 0.0
blast CAB02790 Hypothetical protein C27A7.5c [Caenorhabditis elegans] ref|NP_506048.1| C27A7.5c [Caenorhabditis elegans] 0.0
blast CAE75404 Hypothetical protein CBG23394 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006034 EMBL-EBI Asparaginase/glutaminase 7.7E-55
Pfam IPR002110 EMBL-EBI Ankyrin 10.0
Pfam IPR002110 EMBL-EBI Ankyrin 5.7E-8
Pfam IPR002110 EMBL-EBI Ankyrin 1.2E-8
Pfam IPR006034 EMBL-EBI Asparaginase/glutaminase 7.7E-55
Pfam IPR002110 EMBL-EBI Ankyrin 10.0
Pfam IPR002110 EMBL-EBI Ankyrin 5.7E-8
Pfam IPR002110 EMBL-EBI Ankyrin 1.2E-8
Pfam IPR006034 EMBL-EBI Asparaginase/glutaminase 7.7E-55
Pfam IPR002110 EMBL-EBI Ankyrin 10.0
Pfam IPR002110 EMBL-EBI Ankyrin 5.7E-8
Pfam IPR002110 EMBL-EBI Ankyrin 1.2E-8
Sequence
Target Sequence 
>C. ELEGANS:191174_S_AT
caattgctccgtgacatcattcttccaccaatgttttgtaatgctgctaagacgaatgac
gtagaaattctgaaatcgctgaaagcagccggagtcaatttctcggcaactgattacaat
cttcgaactgctcttcatgttgctgcctccaatggacatctcgaatctgtcaattattta
cttaagataggaacgaatgttcacatcaaagatatgtttggatataatgcacttgtgtgt
gcagtgaaagcaaaagccatggactgtatcattgcgatcagagacgctggtggattcatt
gatgcttcagctcaaaaaattggtgtggaactttgtcttgctgtgtacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATTGCTCCGTGACATCATTCTTC 665 179 1534 Antisense
ATCATTCTTCCACCAATGTTTTGTA 363 31 1549 Antisense
GAAAGCAGCCGGAGTCAATTTCTCG 541 359 1614 Antisense
GTCAATTTCTCGGCAACTGATTACA 488 463 1627 Antisense
GATTACAATCTTCGAACTGCTCTTC 14 431 1645 Antisense
ATCTTCGAACTGCTCTTCATGTTGC 472 31 1652 Antisense
TGCTGCCTCCAATGGACATCTCGAA 564 581 1674 Antisense
AGCCATGGACTGTATCATTGCGATC 220 83 1788 Antisense
GCGATCAGAGACGCTGGTGGATTCA 118 291 1807 Antisense
GGTGGATTCATTGATGCTTCAGCTC 203 499 1822 Antisense
GTGGAACTTTGTCTTGCTGTGTACC 393 491 1858 Antisense
Sequence Source Affymetrix Proprietary Database