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[191165_at]

Description F59A6.1 /REP_DB=WormBase Gene ID /WP=CE27177 /GEN=nsy-1 /TR=Q21029 /GB=AAK31527.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Protein kinase
Cluster 6grp_highest_10_10 > 83      6grp_high1_10_10 > 38      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 84      6grp_total > 37      4grp_total > 13      6grp_highest_sub > Cu      6grp_cgc > 16      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 806
1 2 3 4 range
A 815.8
(-0.3)
P 		648.6
(-1.7)
P 		492.7
(-3)
P 		1214.3
(3)
P 		722
B 885.7
(-0.1)
P 		585
(-2.3)
P 		490.6
(-3)
P 		1296.9
(3)
P 		806
C 836.8
(0.7)
P 		569.7
(-3)
P 		572
(-3)
P 		998.1
(3)
P 		428
avr 846.1
(0) 		601.1
(-2.2) 		518.4
(-3) 		1169.8
(3) 		651

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 639
1 2 3 4 5 6 range
A 742.7
(1.4)
P 		890.5
(3)
P 		321.3
(-3)
P 		615.5
(0.1)
P 		383.8
(-2.3)
P 		884.5
(2.9)
P 		569
B 461.7
(0.4)
P 		523.8
(2.5)
P 		360.5
(-2.9)
P 		495.6
(1.6)
P 		358.5
(-3)
P 		538.1
(3)
P 		180
C 959.8
(3)
P 		487.8
(-2.4)
P 		700.6
(0)
P 		439.1
(-3)
P 		721.2
(0.3)
P 		614.1
(-1)
P 		521
avr 721.4
(3) 		634
(1) 		460.8
(-3) 		516.7
(-1.7) 		487.8
(-2.4) 		678.9
(2) 		261
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 183
1 2 3 4 range
A 297
(3)
P 		140.8
(-2.6)
P 		128.8
(-3)
P 		177.7
(-1.3)
P 		168
B 282.4
(3)
P 		144.5
(-1.9)
P 		113.7
(-3)
P 		276.4
(2.8)
P 		169
C 277
(3)
P 		163.8
(-3)
P 		199.4
(-1.1)
P 		168.3
(-2.8)
P 		113
avr 285.5
(3) 		149.7
(-2.9) 		147.3
(-3) 		207.5
(-0.4) 		138

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 286
1 2 3 4 5 6 range
A 136.9
(-1.4)
P 		316.7
(3)
P 		70
(-3)
P 		156.1
(-0.9)
P 		115.8
(-1.9)
P 		215.4
(0.5)
P 		247
B 160.6
(0.9)
P 		174.3
(1.6)
P 		87.5
(-3)
P 		131.3
(-0.7)
P 		145.7
(0.1)
P 		200.7
(3)
P 		113
C 355.8
(3)
P 		148.8
(-1.6)
P 		178.3
(-1)
P 		88.3
(-3)
P 		233.4
(0.3)
P 		205.6
(-0.4)
P 		268
avr 217.8
(3) 		213.3
(2.7) 		111.9
(-3) 		125.2
(-2.2) 		165
(0) 		207.2
(2.4) 		106
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191165_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4884
Sequence Type Exemplar sequence
Representative Public ID F59A6.1 NCBI
Target Description F59A6.1 /REP_DB=WormBase Gene ID /WP=CE27177 /GEN=nsy-1 /TR=Q21029 /GB=AAK31527.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062524
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000025572 ENSEMBL cdna:SNAP chromosome:CEL140:II:5022897:5029576:1 10/11 None
GENEFINDER00000025582 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5022897:5029576:1 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_062524 7/11 Cross Hyb Matching Probes None
F59A6.1 7/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5022894-5028969(+) 100.0 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK31527 Neuronal symmetry protein 1 [Caenorhabditis elegans] ref|NP_494925.2| Neuronal Symmetry family member (nsy-1) [Caenorhabditis elegans] 0.0
blast T16511 hypothetical protein F59A6.1 - Caenorhabditis elegans 0.0
blast CAE59022 Hypothetical protein CBG02299 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-14
hanks 2.1.17 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; TWITCH 3.0E-66
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.1E-80
Sequence
Target Sequence 
>C. ELEGANS:191165_AT
ttggccagataaaccttgctctctaccacttctccgattcaattcaacctgttctccgtc
gccttgatataaagcctcattggatgtttgcactttcaaatctaatcacttcagccgttc
aatgtgccatttcaattctaagtccagatctttctctcctacttcatgctcaagataatc
ttccatccacatcatcaattgttgcaatcagaaattcctcactttctgaaggtgaagctc
tcattgaatctcgacctccaagtcgtgaagaacgagttcgtgaggatcgaaaagaattga
gaactttacaagaagaaaatgaaattctaattgaacgattgcttcaagtggaaagagaat
taaatgctcaactcaaatccggaatcaccagagccaatcgtttccgagattttgccatgt
accgtaacacatatccaccattccgtactccaccagtcgctcatgctcccccaactcctc
cattcagtgcttcatgtggtgctcaaccgtcaggaaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGCCAGATAAACCTTGCTCTCTA 341 709 3578 Antisense
TACCACTTCTCCGATTCAATTCAAC 72 651 3601 Antisense
TGTTCTCCGTCGCCTTGATATAAAG 460 561 3627 Antisense
AAATCTAATCACTTCAGCCGTTCAA 6 119 3675 Antisense
ATTCTAAGTCCAGATCTTTCTCTCC 285 11 3712 Antisense
ATCTTCCATCCACATCATCAATTGT 469 31 3755 Antisense
GTGAAGCTCTCATTGAATCTCGACC 548 481 3809 Antisense
AAATCCGGAATCACCAGAGCCAATC 671 117 3952 Antisense
CGTTTCCGAGATTTTGCCATGTACC 90 245 3976 Antisense
TCCTCCATTCAGTGCTTCATGTGGT 707 593 4053 Antisense
ATGTGGTGCTCAACCGTCAGGAACA 127 51 4071 Antisense
Sequence Source Affymetrix Proprietary Database