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[191161_at]

Description C32F10.8 /REP_DB=WormBase Gene ID /WP=CE08545 /TR=O01685 /GB=AAC24265.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=alanine aminotransferase
Cluster 6grp_highest_10_10 > 55      6grp_high1_10_10 > 44      4grp_highest_8_8 > 42      4grp_high_8_8 > 49      6grp_highest_nonorm > 47      6grp_total > 30      4grp_total > 63      6grp_high1_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5125
1 2 3 4 range
A 4468.4
(3)
P 		2302.3
(-0.8)
P 		2864.2
(0.2)
P 		1003.1
(-3)
P 		3465
B 6127.7
(3)
P 		2862.4
(-1.4)
P 		2825.8
(-1.4)
P 		1669.3
(-3)
P 		4458
C 5150.3
(3)
P 		2198.6
(-1.4)
P 		2970.3
(-0.2)
P 		1109.7
(-3)
P 		4041
avr 5248.8
(3) 		2454.4
(-1.2) 		2886.8
(-0.6) 		1260.7
(-3) 		3988

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3015
1 2 3 4 5 6 range
A 2941.9
(-2.8)
P 		4050.4
(0.4)
P 		3856.9
(-0.2)
P 		4956.2
(3)
P 		2884.5
(-3)
P 		3934.3
(0)
P 		2072
B 3423.1
(0.2)
P 		3963.2
(3)
P 		2818.4
(-3)
P 		3111.7
(-1.5)
P 		3727.4
(1.8)
P 		3684.2
(1.5)
P 		1145
C 2572.8
(-2)
P 		4253.2
(1.3)
P 		5093.2
(3)
P 		4717.8
(2.3)
P 		2077.8
(-3)
P 		3917.6
(0.7)
P 		3015
avr 2979.3
(-2.6) 		4088.9
(2.2) 		3922.8
(1.5) 		4261.9
(3) 		2896.6
(-3) 		3845.4
(1.2) 		1365
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1807
1 2 3 4 range
A 1626.9
(3)
P 		499.8
(-1.6)
P 		748.7
(-0.6)
P 		146.8
(-3)
P 		1480
B 1954.1
(3)
P 		707
(-1.7)
P 		654.8
(-1.9)
P 		355.7
(-3)
P 		1598
C 1704.9
(3)
P 		632.1
(-1.2)
P 		1035.3
(0.4)
P 		187.1
(-3)
P 		1518
avr 1762
(3) 		613
(-1.5) 		812.9
(-0.7) 		229.9
(-3) 		1532

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 972
1 2 3 4 5 6 range
A 542.3
(-3)
P 		1440.5
(3)
P 		840
(-1)
P 		1257
(1.8)
P 		870.4
(-0.8)
P 		957.9
(-0.2)
P 		898
B 1191.1
(0.7)
P 		1319.2
(1.6)
P 		684.4
(-3)
P 		824.2
(-2)
P 		1514.4
(3)
P 		1373.8
(2)
P 		830
C 953.6
(-0.4)
P 		1297.8
(2.9)
P 		1296.3
(2.9)
P 		948.8
(-0.4)
P 		672.4
(-3)
P 		1311.4
(3)
P 		639
avr 895.7
(-3) 		1352.5
(3) 		940.2
(-2.4) 		1010
(-1.5) 		1019.1
(-1.4) 		1214.4
(1.2) 		457
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191161_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2405
Sequence Type Exemplar sequence
Representative Public ID C32F10.8 NCBI
Target Description C32F10.8 /REP_DB=WormBase Gene ID /WP=CE08545 /TR=O01685 /GB=AAC24265.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=alanine aminotransferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001025850, NM_001025851
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
C32F10.8b.1 ENSEMBL cdna:known chromosome:CEL140:I:5830977:5834284:-1 gene:C32F10.8 A 
C32F10.8b.2 ENSEMBL cdna:known chromosome:CEL140:I:5830977:5834291:-1 gene:C32F10.8 A 
C32F10.8b.3 ENSEMBL cdna:known chromosome:CEL140:I:5830977:5833210:-1 gene:C32F10.8 A 
C32F10.8a ENSEMBL cdna:known chromosome:CEL140:I:5831120:5833209:-1 gene:C32F10.8 None
C32F10.8b.5 ENSEMBL cdna:known chromosome:CEL140:I:5831760:5834283:-1 gene:C32F10.8 None
C32F10.8b.4 ENSEMBL cdna:known chromosome:CEL140:I:5831760:5833223:-1 gene:C32F10.8 None
NM_001025851 NCBI Caenorhabditis elegans C32F10.8b (C32F10.8) mRNA, complete cds. None
NM_001025850 NCBI Caenorhabditis elegans C32F10.8a (C32F10.8) mRNA, complete cds. None
SNAP00000021238 ENSEMBL cdna:SNAP chromosome:CEL140:I:5831274:5833209:-1 None
GENEFINDER00000021247 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5831274:5833209:-1 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5830950-5833209(-) 100.0 100.0
Public Domain and Genome References
Ensembl C32F10.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAO61435 Hypothetical protein C32F10.8b [Caenorhabditis elegans] ref|NP_001021022.1| C32F10.8b [Caenorhabditis elegans] 0.0
blast AAC24265 Hypothetical protein C32F10.8a [Caenorhabditis elegans] ref|NP_001021021.1| C32F10.8a [Caenorhabditis elegans] 0.0
blast CAE60451 Hypothetical protein CBG04059 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec AAT_ECOLI (P00509) Aspartate aminotransferase (EC 2.6.1.1) (Transaminase A) (ASPAT) 0.059
ec 1A11_CUCMA (P23599) 1-aminocyclopropane-1-carboxylate synthase CMW33 (EC 4.4.1.14) (ACC synthase) (S-adenosyl-L-methionine methylthioadenosine-lyase) 9.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR004839 EMBL-EBI Aminotransferase, class I and II 1.7E-20
Sequence
Target Sequence 
>C. ELEGANS:191161_AT
tgatctctgccaaattgtgctccacagtactcggacaagccgtcattgatgccgttgtga
atccaccaaaggaaggagatgcatcgtatgctctgtggaaacaagaaaaggatgcagtac
ttgcttctcttaaggaacgtgctacactcgtcgagaaggcatacagtagcattgatggaa
tcagctgtaatccagttcaaggagccatgtacgctttcccgcaaatcacgattccacaga
gagctgttgaaaaagctcagtctttgaaccaacaacctgatttcttctatgctatgcaac
ttcttgagaccaccggaatctgtatcgtgccaggaagtggatttggacaaaaagacggaa
cttatcatttcagaacgacaattcttccacagccagaactcttcaaagacatgctctccc
ggttcactgatttccaccaaaaattccttgccgaatacaaaagactatactgccactttc
acctgacaaacaataggacctcagacagtttctggcacactcttattgatggattcccaa
aatacaaggtctttgtccagaaccgcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGATCTCTGCCAAATTGTGCTCCAC 630 565 1064 Antisense
TGTGCTCCACAGTACTCGGACAAGC 700 555 1079 Antisense
CGGACAAGCCGTCATTGATGCCGTT 74 243 1095 Antisense
GGATGCAGTACTTGCTTCTCTTAAG 280 513 1173 Antisense
GAACGTGCTACACTCGTCGAGAAGG 532 347 1198 Antisense
TTCAAGGAGCCATGTACGCTTTCCC 313 683 1259 Antisense
TTTCCCGCAAATCACGATTCCACAG 676 669 1278 Antisense
AAAGACTATACTGCCACTTTCACCT 557 121 1523 Antisense
GGACCTCAGACAGTTTCTGGCACAC 585 523 1559 Antisense
GGCACACTCTTATTGATGGATTCCC 392 535 1577 Antisense
TACAAGGTCTTTGTCCAGAACCGCC 293 639 1606 Antisense
Sequence Source Affymetrix Proprietary Database