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[191125_s_at] Heat shock hsp70 protein

Description F11F1.1A /REP_DB=WormBase Gene ID /WP=CE15802 /TR=O45350 /GB=CAB03283.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Heat shock hsp70 proteins
Cluster 6grp_high1_10_10 > 80      4grp_high_8_8 > 37      6grp_total > 82      4grp_total > 0      6grp_high1_sub > 02_03_04_05      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 43
1 2 3 4 range
A 50.3
(-0)
P 		35
(-3)
A 		41.8
(-1.7)
A 		65.7
(3)
P 		31
B 58.5
(-2.5)
P 		77.8
(3)
P 		64.1
(-0.9)
P 		56.7
(-3)
P 		21
C 55.5
(0)
P 		41.2
(-3)
M 		55.9
(0.1)
P 		69.6
(3)
P 		28
avr 54.8
(-1.3) 		51.3
(-3) 		53.9
(-1.8) 		64
(3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 81
1 2 3 4 5 6 range
A 65.5
(-0.2)
M 		74.1
(0.6)
P 		95.3
(2.5)
P 		71.2
(0.3)
P 		100.8
(3)
P 		34.4
(-3)
A 		66
B 59.9
(-2)
P 		99.9
(1.9)
P 		49.2
(-3)
M 		111.4
(3)
P 		89.1
(0.8)
P 		50.3
(-2.9)
P 		62
C 58.8
(-1.4)
P 		65.5
(-0.9)
P 		87.2
(0.8)
P 		97.9
(1.6)
P 		115.3
(3)
P 		38.1
(-3)
P 		77
avr 61.4
(-1) 		79.8
(0.8) 		77.2
(0.6) 		93.5
(2.2) 		101.7
(3) 		40.9
(-3) 		61
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 18.3
(3)
P 		7.6
(-3)
A 		10.9
(-1.1)
A 		9.6
(-1.9)
P 		11
B 18.6
(2.5)
P 		19.2
(3)
P 		14.9
(-0.6)
P 		12.1
(-3)
P 		7
C 18.4
(2.2)
P 		11.8
(-2.9)
M 		19.5
(3)
P 		11.7
(-3)
P 		8
avr 18.4
(3) 		12.9
(-1.5) 		15.1
(0.3) 		11.1
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 29
1 2 3 4 5 6 range
A 12.1
(-2)
M 		26.4
(1.9)
P 		20.7
(0.4)
P 		18.1
(-0.4)
P 		30.4
(3)
P 		8.4
(-3)
A 		22
B 20.8
(-0.8)
P 		33.2
(2.3)
P 		12
(-3)
M 		29.5
(1.3)
P 		36.2
(3)
P 		18.8
(-1.3)
P 		24
C 21.8
(-0.8)
P 		20
(-1.2)
P 		22.2
(-0.7)
P 		19.7
(-1.3)
P 		37.3
(3)
P 		12.7
(-3)
P 		25
avr 18.2
(-1.6) 		26.5
(0.7) 		18.3
(-1.6) 		22.4
(-0.4) 		34.6
(3) 		13.3
(-3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191125_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6321
Sequence Type Exemplar sequence
Representative Public ID F11F1.1A NCBI
Target Description F11F1.1A /REP_DB=WormBase Gene ID /WP=CE15802 /TR=O45350 /GB=CAB03283.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Heat shock hsp70 proteins
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026078(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026078 NCBI Caenorhabditis elegans F11F1.1 (F11F1.1) mRNA, complete cds. 11/11 None
SNAP00000021811 ENSEMBL cdna:SNAP chromosome:CEL140:III:13398415:13400036:1 10/11 None
GENEFINDER00000021820 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:13398415:13400036:1 10/11 None
F11F1.1b ENSEMBL cdna:known chromosome:CEL140:III:13395240:13400036:1 gene:F11F1.1 11/11 None
F11F1.1a ENSEMBL cdna:known chromosome:CEL140:III:13395240:13400036:1 gene:F11F1.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000021808 1/11 Cross Hyb Matching Probes None
GENEFINDER00000021819 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:13395235-13400032(+) 100.0 100.0
Public Domain and Genome References
Gene Title Heat shock hsp70 protein
Ensembl F11F1.1
Entrez Gene 3565569 Entrez gene
SwissProt Q9TW52 EMBL-EBI
WormBase CE23645 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB54300 Hypothetical protein F11F1.1 [Caenorhabditis elegans] emb|CAB54217.1| Hypothetical protein F11F1.1 [Caenorhabditis elegans] ref|NP_001021249.1| F11F1.1 [Caenorhabditis elegans] 0.0
blast CAE62854 Hypothetical protein CBG07033 [Caenorhabditis briggsae] 0.0
blast AAY33973 HSP70 [Oxyuranus scutellatus scutellatus] 1.0E-119
blast AAY33973 HSP70 [Oxyuranus scutellatus scutellatus] 1.0E-117
blast CAB54300 Hypothetical protein F11F1.1 [Caenorhabditis elegans] emb|CAB54217.1| Hypothetical protein F11F1.1 [Caenorhabditis elegans] ref|NP_001021249.1| F11F1.1 [Caenorhabditis elegans] 1.0E-91
Protein Domains
Database ID Description E-Value
Pfam IPR001023 EMBL-EBI Heat shock protein Hsp70 1.0E-126
Pfam IPR001023 EMBL-EBI Heat shock protein Hsp70 1.0E-126
Pfam IPR001023 EMBL-EBI Heat shock protein Hsp70 1.9E-12
Pfam IPR003677 EMBL-EBI Protein of unknown function, DUF 148 3.1E-39
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000021811 1 7-26
GENEFINDER00000021820 1 7-26
Sequence
Target Sequence 
>C. ELEGANS:191125_S_AT
gaacggttttcccatgtacaaagaggaagactttcatcaatagtgatgattttcagacat
caatcaatgttccagtttacgaaggagaacgagtgttgtgttcacacaaccgccaactcg
gagaaataagtctaccaattcaaccaaaaagacgtggtgaatcagtagttgatatcacat
tggaagtagattataatggaattcttcaagcaactgcacttgatttaaacacaaaaaagg
caataaccacaacaattttatacgattgttgtacttatactcaaccggaaattgatgaaa
tgactagccaaagcgaagaagatcgaatctttgatgagcatttcaaaatgcgatacaaac
aattacaaaaaagtgaagacatggcatatggttataaacatcgattagagaaagttaagg
tacataaatatgaagcactagggagcgttgagcctggaaaattctcgaacttgatggaaa
cagtggaacgagaaatttcatggttgtctgtattcccaaaagtgtctcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACGGTTTTCCCATGTACAAAGAG 35 347 1250 Antisense
GGAGAACGAGTGTTGTGTTCACACA 422 519 1333 Antisense
TTCACACAACCGCCAACTCGGAGAA 182 687 1350 Antisense
TAAGTCTACCAATTCAACCAAAAAG 579 637 1376 Antisense
ATGGAATTCTTCAAGCAACTGCACT 324 53 1445 Antisense
GCAACTGCACTTGATTTAAACACAA 462 319 1459 Antisense
GATTGTTGTACTTATACTCAACCGG 294 435 1513 Antisense
GATCGAATCTTTGATGAGCATTTCA 613 417 1570 Antisense
GGGAGCGTTGAGCCTGGAAAATTCT 641 493 1690 Antisense
AAATTTCATGGTTGTCTGTATTCCC 89 117 1742 Antisense
GTCTGTATTCCCAAAAGTGTCTCCA 333 471 1755 Antisense
Sequence Source Affymetrix Proprietary Database