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[191119_s_at]

Description F35G12.4B /REP_DB=WormBase Gene ID /WP=CE20751 /TR=Q9U3G8 /GB=CAA86338.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=WD domain, G-beta repeat
Cluster 6grp_high1_10_10 > 87      6grp_total > 87      4grp_total > 59      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 178
1 2 3 4 range
A 212.1
(0.9)
P 		175.8
(-2.2)
A 		236.4
(3)
A 		166.5
(-3)
A 		70
B 163.1
(-0.8)
P 		212.6
(3)
P 		155.5
(-1.4)
P 		135.4
(-3)
A 		77
C 201.1
(1.2)
P 		232.1
(2.2)
A 		255.6
(3)
P 		77.2
(-3)
M 		178
avr 192.1
(1.4) 		206.8
(2.4) 		215.8
(3) 		126.4
(-3) 		89

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 421
1 2 3 4 5 6 range
A 255.2
(-1.9)
M 		205.9
(-2.6)
P 		182.5
(-3)
P 		376.7
(0)
P 		383.1
(0.1)
P 		568.6
(3)
P 		386
B 281.6
(-1.5)
P 		269.4
(-1.7)
P 		177
(-3)
P 		291.1
(-1.4)
P 		342
(-0.6)
P 		597.5
(3)
P 		421
C 284
(-0.9)
P 		230.1
(-3)
P 		229
(-3)
P 		284.5
(-0.9)
P 		369.5
(2.2)
P 		389.8
(3)
P 		161
avr 273.6
(-1.6) 		235.1
(-2.3) 		196.2
(-3) 		317.4
(-0.7) 		364.9
(0.1) 		518.6
(3) 		322
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 77.2
(3)
P 		38.2
(-1.4)
A 		61.8
(1.3)
A 		24.4
(-3)
A 		53
B 52
(2.9)
P 		52.5
(3)
P 		36
(-1.2)
P 		28.8
(-3)
A 		24
C 66.6
(1.2)
P 		66.7
(1.2)
A 		89.1
(3)
P 		13
(-3)
M 		76
avr 65.3
(3) 		52.5
(1.2) 		62.3
(2.6) 		22.1
(-3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 183
1 2 3 4 5 6 range
A 47.1
(-2.6)
M 		73.2
(-1)
P 		39.7
(-3)
P 		95.5
(0.4)
P 		115.6
(1.6)
P 		138.4
(3)
P 		99
B 98
(-1.2)
P 		89.7
(-1.4)
P 		43
(-3)
P 		77.1
(-1.9)
P 		138.9
(0.2)
P 		222.8
(3)
P 		180
C 105.3
(0.9)
P 		70.2
(-1.9)
P 		58.3
(-2.9)
P 		57.2
(-3)
P 		119.6
(2.1)
P 		130.5
(3)
P 		73
avr 83.5
(-1.1) 		77.7
(-1.4) 		47
(-3) 		76.6
(-1.5) 		124.7
(1) 		163.9
(3) 		117
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191119_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7168
Sequence Type Exemplar sequence
Representative Public ID F35G12.4B NCBI
Target Description F35G12.4B /REP_DB=WormBase Gene ID /WP=CE20751 /TR=Q9U3G8 /GB=CAA86338.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=WD domain, G-beta repeat
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065530(11), NM_065529(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065530 NCBI Caenorhabditis elegans F35G12.4b (beta-transducin) mRNA, complete cds. 11/11 None
NM_065529 NCBI Caenorhabditis elegans F35G12.4a (beta-transducin) mRNA, complete cds. 11/11 None
SNAP00000042238 ENSEMBL cdna:SNAP chromosome:CEL140:III:4578847:4581530:1 11/11 None
GENEFINDER00000042264 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4578847:4581530:1 11/11 None
F35G12.4a.2 ENSEMBL cdna:known chromosome:CEL140:III:4578818:4581642:1 gene:F35G12.4 11/11 None
F35G12.4a.1 ENSEMBL cdna:known chromosome:CEL140:III:4578820:4581642:1 gene:F35G12.4 11/11 None
F35G12.4b ENSEMBL cdna:known chromosome:CEL140:III:4578847:4581647:1 gene:F35G12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4578846-4581530(+) 100.0 100.0
Public Domain and Genome References
Ensembl F35G12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA86338 Hypothetical protein F35G12.4b [Caenorhabditis elegans] ref|NP_497931.2| F35G12.4b [Caenorhabditis elegans] 0.0
blast CAA86335 Hypothetical protein F35G12.4a [Caenorhabditis elegans] ref|NP_497930.2| F35G12.4a [Caenorhabditis elegans] sp|Q20059|YPR4_CAEEL Hypothetical WD-repeat protein F35G12.4 in chromosome III 0.0
blast T21810 hypothetical protein F35G12.4b - Caenorhabditis elegans 0.0
blast T21808 hypothetical protein F35G12.4a - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.046
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.1E-8
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.1E-4
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.022
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.8E-9
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.031
Sequence
Target Sequence 
>C. ELEGANS:191119_S_AT
gaatgatgctacgatcactcttcgaacgatggccaccttgtaaaatgacaaatgttgatg
cagctgatgcagatgatgtgcaaaaagcaacactgaattttatttctctaccggaacaca
caccgcttattatttgcgaaggaaatggtcgtccgctctaccgtcttcttgttggcgacg
ctgggaaggagttcgaagcaaatgagctggcacaaattgcacccatgtgggttatagacg
caatcgaacgaaatcagctgccgaaattcaataaaatgccattttatcttcttccacatc
cgtcgacaaatcccaaacagcctaaaaaagatcgtctgtcagcaactgaaatgcttcaag
ttaagaaagtgatggagcatgtttacgaaaaaatactcagcactaatgatgttggctcga
ttccactaaatcaaattcatacaaaaatggaaatgtactgcaatgatcagaggttggagc
cggatatggatttgcgaacagtcaagcatctctattgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAATGATGCTACGATCACTCTTCGA 559 331 1502 Antisense
TCTTCGAACGATGGCCACCTTGTAA 416 617 1520 Antisense
TTATTTCTCTACCGGAACACACACC 323 679 1601 Antisense
GGAACACACACCGCTTATTATTTGC 662 527 1614 Antisense
CGAAGGAAATGGTCGTCCGCTCTAC 526 253 1638 Antisense
TGTTGGCGACGCTGGGAAGGAGTTC 442 553 1671 Antisense
GAGCTGGCACAAATTGCACCCATGT 501 387 1705 Antisense
GAACGAAATCAGCTGCCGAAATTCA 562 343 1747 Antisense
TCGACAAATCCCAAACAGCCTAAAA 581 601 1804 Antisense
GTTGGCTCGATTCCACTAAATCAAA 281 435 1912 Antisense
GCGAACAGTCAAGCATCTCTATTGG 445 287 1995 Antisense
Sequence Source Affymetrix Proprietary Database