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[191107_s_at]

Description K04F10.4C /REP_DB=WormBase Gene ID /WP=CE11732 /GEN=bli-4 /TR=O44765 /GB=AAB96757.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=endoprotease
Cluster 6grp_highest_10_10 > 03      6grp_high1_10_10 > 11      4grp_highest_8_8 > 03      4grp_high_8_8 > 38      6grp_highest_nonorm > 71      6grp_total > 22      4grp_total > 41      6grp_highest_sub > De      6grp_cgc > 61      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 166
1 2 3 4 range
A 239.1
(-3)
P 		296.4
(2.3)
P 		303.5
(3)
P 		292.2
(1.9)
P 		64
B 211.8
(-1.8)
P 		265.1
(2.3)
P 		274.3
(3)
P 		196.8
(-3)
P 		78
C 137.4
(-3)
P 		265.9
(3)
P 		215.5
(0.6)
P 		237.4
(1.7)
P 		129
avr 196.1
(-3) 		275.8
(3) 		264.4
(2.1) 		242.1
(0.5) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 282
1 2 3 4 5 6 range
A 268.4
(-1.3)
P 		359.7
(1.1)
P 		321.5
(0.1)
P 		433.4
(3)
P 		269.7
(-1.2)
P 		201.8
(-3)
P 		232
B 308.3
(0.6)
P 		384.7
(3)
P 		361.8
(2.3)
P 		259.5
(-1)
P 		197.2
(-3)
P 		226.5
(-2.1)
P 		188
C 478.9
(3)
P 		328.8
(-0.9)
P 		377.4
(0.4)
P 		309.9
(-1.4)
P 		352.7
(-0.3)
P 		247.6
(-3)
P 		231
avr 351.9
(2.7) 		357.7
(3) 		353.6
(2.8) 		334.3
(1.9) 		273.2
(-0.8) 		225.3
(-3) 		132
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 87.1
(3)
P 		64.3
(-0.1)
P 		79.4
(2)
P 		42.8
(-3)
P 		44
B 67.5
(3)
P 		65.5
(2.5)
P 		63.6
(2.1)
P 		41.9
(-3)
P 		26
C 45.5
(-2.1)
P 		76.4
(3)
P 		75.1
(2.8)
P 		40
(-3)
P 		36
avr 66.7
(1.8) 		68.7
(2.2) 		72.7
(3) 		41.6
(-3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 128
1 2 3 4 5 6 range
A 49.5
(-3)
P 		127.9
(3)
P 		70
(-1.4)
P 		109.9
(1.6)
P 		81.4
(-0.5)
P 		49.1
(-3)
P 		79
B 107.3
(0.9)
P 		128
(3)
P 		87.9
(-1.1)
P 		68.7
(-3)
P 		80.1
(-1.8)
P 		84.5
(-1.4)
P 		59
C 177.5
(3)
P 		100.3
(-1)
P 		96.1
(-1.2)
P 		62.3
(-3)
P 		114.1
(-0.3)
P 		82.9
(-1.9)
P 		115
avr 111.4
(2.1) 		118.7
(3) 		84.7
(-1.4) 		80.3
(-2) 		91.9
(-0.5) 		72.2
(-3) 		47
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191107_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1846
Sequence Type Exemplar sequence
Representative Public ID K04F10.4C NCBI
Target Description K04F10.4C /REP_DB=WormBase Gene ID /WP=CE11732 /GEN=bli-4 /TR=O44765 /GB=AAB96757.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=endoprotease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026372(11), NM_001026371(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026372 NCBI Caenorhabditis elegans K04F10.4d (endoprotease) mRNA, complete cds. 11/11 None
NM_001026371 NCBI Caenorhabditis elegans K04F10.4c (endoprotease) mRNA, complete cds. 11/11 None
GENEFINDER00000027825 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6360824:6364593:1 11/11 None
K04F10.4d ENSEMBL cdna:known chromosome:CEL140:I:6350572:6364813:1 gene:K04F10.4 11/11 A 
K04F10.4c ENSEMBL cdna:known chromosome:CEL140:I:6350572:6363567:1 gene:K04F10.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000027820 7/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172333 6 2 NM_001026369 175669_at (11)
NM_001026370 175666_s_at (11)
NM_001026371 191107_s_at (11)
NM_001026372 172006_x_at (11), 191062_s_at (11), 191107_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6350500-6363496(+) 94.81 100.0
Public Domain and Genome References
Ensembl K04F10.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB96754 Blistered cuticle protein 4, isoform d [Caenorhabditis elegans] ref|NP_001021543.1| K04F10.4d [Caenorhabditis elegans] pir||D87803 protein bli-4D [imported] - Caenorhabditis elegans 0.0
blast P51559 Endoprotease bli-4 precursor (Blisterase) (Blistered cuticle protein 4) 0.0
blast AAB96757 Blistered cuticle protein 4, isoform c [Caenorhabditis elegans] ref|NP_001021542.1| K04F10.4c [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002884 EMBL-EBI Peptidase S8B, proprotein convertase, P domain 2.9E-70
Pfam IPR000209 EMBL-EBI Peptidase S8 and S53, subtilisin, kexin, sedolisin 2.2E-113
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021543.1 1 873-892
K04F10.4d 1 873-892
GENEFINDER00000027825 1 388-407
Sequence
Target Sequence 
>C. ELEGANS:191107_S_AT
gttgaagagtctgctccgatctcatttccagatttgacgtcggctggaaattgtcatgat
gaatgtaatggaggttgcacagaatcgagctcagcaacatcatgtttcgcttgcaaacat
ttaactcaaactcttcgaaataaaggaggaagtggattcaagtgtgttcaaaaatgcgat
gatacttactacttggatggtgacaagtgtaaaatgtgctcatcccattgtcatacttgt
acgaaagctgaggtgtgtgaaacgtgtcctggaagtcttttgctcattgatgtggataat
atgccacactatgatcatggaaagtgtgttgagtcatgtcctccaggattggttgctgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTGAAGAGTCTGCTCCGATCTCAT 481 439 1984 Antisense
CTCATTTCCAGATTTGACGTCGGCT 516 227 2004 Antisense
ACGTCGGCTGGAAATTGTCATGATG 511 95 2020 Antisense
GAGGTTGCACAGAATCGAGCTCAGC 158 403 2054 Antisense
GAGCTCAGCAACATCATGTTTCGCT 404 387 2070 Antisense
ATGCGATGATACTTACTACTTGGAT 684 41 2157 Antisense
AAATGTGCTCATCCCATTGTCATAC 130 121 2195 Antisense
GTGTGTGAAACGTGTCCTGGAAGTC 221 487 2236 Antisense
GGAAGTCTTTTGCTCATTGATGTGG 674 531 2254 Antisense
AAGTGTGTTGAGTCATGTCCTCCAG 529 159 2305 Antisense
ATGTCCTCCAGGATTGGTTGCTGAT 283 49 2319 Antisense
Sequence Source Affymetrix Proprietary Database