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[191101_at] 7TM chemoreceptor, srh family

Description ZC404.5 /REP_DB=WormBase Gene ID /WP=CE25664 /GEN=srh-28 /TR=Q23284 /GB=AAA97961.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster 4grp_high_8_8 > 13      6grp_total > 21      4grp_total > 29      6grp_cgc > 61      6grp_cgc_sub > De      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 90
1 2 3 4 range
A 68.8
(1.2)
P 		84.3
(3)
P 		32.1
(-3)
A 		53
(-0.6)
A 		52
B 77.3
(3)
P 		61.9
(1.3)
P 		23.5
(-3)
A 		57.1
(0.7)
M 		54
C 78.7
(-1.1)
P 		67.3
(-2.5)
A 		63.2
(-3)
P 		113.1
(3)
P 		50
avr 74.9
(3) 		71.2
(2.4) 		39.6
(-3) 		74.4
(2.9) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 35.6
(0)
A 		54.2
(2.1)
P 		61.7
(3)
P 		28.5
(-0.8)
A 		48.3
(1.5)
A 		9.2
(-3)
A 		53
B 50.5
(3)
P 		31.1
(-0.3)
A 		18.8
(-2.3)
A 		36.1
(0.6)
A 		34
(0.2)
P 		14.7
(-3)
A 		36
C 23.3
(-1.9)
P 		59.6
(2.9)
P 		60.4
(3)
P 		21.8
(-2.1)
A 		39.8
(0.3)
A 		14.6
(-3)
A 		46
avr 36.5
(1) 		48.3
(3) 		47
(2.8) 		28.8
(-0.3) 		40.7
(1.7) 		12.8
(-3) 		36
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 25.1
(3)
P 		18.3
(0.6)
P 		8.4
(-2.8)
A 		7.8
(-3)
A 		17
B 24.6
(3)
P 		15.3
(0.1)
P 		5.4
(-3)
A 		12.2
(-0.9)
M 		19
C 26.1
(3)
P 		19.4
(-2.7)
A 		22
(-0.5)
P 		19.1
(-3)
P 		7
avr 25.3
(3) 		17.7
(-0.4) 		11.9
(-3) 		13
(-2.5) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 6.6
(-1.5)
A 		19.3
(3)
P 		13.4
(0.9)
P 		7.2
(-1.2)
A 		14.6
(1.4)
A 		2.2
(-3)
A 		17
B 17.6
(3)
P 		10.3
(-0.4)
A 		4.6
(-3)
A 		9.6
(-0.7)
A 		13.8
(1.2)
P 		5.5
(-2.6)
A 		13
C 8.6
(-1.2)
P 		18.2
(3)
P 		15.4
(1.8)
P 		4.4
(-3)
A 		12.9
(0.7)
A 		4.9
(-2.8)
A 		14
avr 10.9
(0.4) 		15.9
(3) 		11.1
(0.5) 		7.1
(-1.5) 		13.8
(1.9) 		4.2
(-3) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191101_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12309
Sequence Type Exemplar sequence
Representative Public ID ZC404.5 NCBI
Target Description ZC404.5 /REP_DB=WormBase Gene ID /WP=CE25664 /GEN=srh-28 /TR=Q23284 /GB=AAA97961.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072314(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072314 NCBI Caenorhabditis elegans Serpentine Receptor, class H family member (srh-28) (srh-28) mRNA, complete cds. 11/11 None
GENEFINDER00000042525 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:6775092:6781860:-1 11/11 None
ZC404.5 ENSEMBL cdna:known chromosome:CEL140:V:6776945:6778227:-1 gene:ZC404.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000042514 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6776945-6778240(-) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, srh family
Gene Symbol srh-28
Ensembl ZC404.5
Entrez Gene 191850 Entrez gene
SwissProt Q23284 EMBL-EBI
WormBase CE38128 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA97961 Serpentine receptor, class h protein 28 [Caenorhabditis elegans] ref|NP_504715.2| Serpentine Receptor, class H family member (srh-28) [Caenorhabditis elegans] 0.0
blast T29366 hypothetical protein ZC404.5 - Caenorhabditis elegans 0.0
blast T29366 hypothetical protein ZC404.5 - Caenorhabditis elegans 1.0E-159
blast AAA97961 Serpentine receptor, class h protein 28 [Caenorhabditis elegans] ref|NP_504715.2| Serpentine Receptor, class H family member (srh-28) [Caenorhabditis elegans] 1.0E-158
blast CAE71948 Hypothetical protein CBG19015 [Caenorhabditis briggsae] 1.0E-108
blast CAE71948 Hypothetical protein CBG19015 [Caenorhabditis briggsae] 2.0E-94
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 1.0E-22
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 2.7E-5
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 7.2E-24
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_504715.2 7 26-48,69-91,106-128,148-170,215-237,258-280,290-312
ZC404.5 7 26-48,69-91,106-128,148-170,215-237,258-280,290-312
GENEFINDER00000042525 9 130-152,173-195,285-307,343-365,380-402,423-445,490-512,533-555,565-587
Sequence
Target Sequence 
>C. ELEGANS:191101_AT
gacttatatttcatccgtgtgctctctttccaattttgcattgctttatgaaaaccagga
taatgcgaaatttttctggtacaaattattggctggaaatatgccagcgaggttttggtc
ggaaagttatgtagtggctagtggcagttactttcggttctccttatttttgaaactctg
cagtattagtcttttcttgtatattattgcttgcattttcattccagtagttgcttttca
aattttaaacagaatgaagcataggctatcacgacatgtggttcaagctcaaaaaatgtc
tatcaaagcactgctgtttcagatatccatcatcgtcagttttttattattcccgttctc
tacttttattgttggcctggaaatgcgagtcaacaacccattggtggttgcaatatctct
tactttcgcaatggtccatggagccgcagcgactttggcaatgatcattgcaaataaacc
acatagaacaattttcctatcgcatctaaaatacggagtttcaattataacgtgttcaat
taattccaaagatgaaaaagcttgccgctccttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTTATATTTCATCCGTGTGCTCT 564 371 489 Antisense
TAGTGGCAGTTACTTTCGGTTCTCC 155 653 627 Antisense
TATTGCTTGCATTTTCATTCCAGTA 348 663 693 Antisense
AGCACTGCTGTTTCAGATATCCATC 663 75 795 Antisense
TATCCATCATCGTCAGTTTTTTATT 141 659 812 Antisense
TCTCTACTTTTATTGTTGGCCTGGA 436 611 845 Antisense
ATGCGAGTCAACAACCCATTGGTGG 683 41 871 Antisense
ATCTCTTACTTTCGCAATGGTCCAT 426 31 903 Antisense
TCCATGGAGCCGCAGCGACTTTGGC 329 587 923 Antisense
GAACAATTTTCCTATCGCATCTAAA 486 353 974 Antisense
AGATGAAAAAGCTTGCCGCTCCTTG 237 67 1038 Antisense
Sequence Source Affymetrix Proprietary Database