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[191075_s_at]

Description B0348.4 /REP_DB=WormBase Gene ID /WP=CE23507 /CHR=5 /FEA=Sanger Annotation /DEF=locus:egl-8 phospholipase (ST.LOUIS) TR:O61956 protein_id:AAC17812.1
Cluster 6grp_high1_10_10 > 03      6grp_total > 25      4grp_total > 30      6grp_cgc > 33      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 351
1 2 3 4 range
A 466.4
(3)
P 		201
(-2.2)
A 		161.1
(-3)
A 		322.4
(0.2)
P 		305
B 428.3
(3)
P 		218
(-1.4)
P 		138.3
(-3)
A 		321.2
(0.8)
P 		290
C 489.4
(3)
P 		207.1
(-2.9)
A 		201.3
(-3)
M 		385.5
(0.8)
P 		288
avr 461.4
(3) 		208.7
(-2.1) 		166.9
(-3) 		343
(0.6) 		295

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 247
1 2 3 4 5 6 range
A 315
(3)
P 		315
(3)
P 		281.6
(1.7)
P 		216.7
(-0.7)
P 		157.6
(-3)
P 		255.8
(0.7)
P 		157
B 235.2
(-1)
P 		380.5
(3)
P 		246.3
(-0.7)
P 		165.2
(-2.9)
P 		203.2
(-1.8)
P 		160.5
(-3)
P 		220
C 382.7
(3)
P 		310.9
(1.3)
P 		265.6
(0.2)
P 		142.3
(-2.8)
A 		190.5
(-1.7)
P 		136.1
(-3)
P 		247
avr 311
(2.1) 		335.5
(3) 		264.5
(0.4) 		174.7
(-3) 		183.8
(-2.7) 		184.1
(-2.6) 		161
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 138
1 2 3 4 range
A 169.8
(3)
P 		43.6
(-2.9)
A 		42.1
(-3)
A 		47.2
(-2.8)
P 		128
B 136.6
(3)
P 		53.8
(-1.8)
P 		32.1
(-3)
A 		68.5
(-0.9)
P 		105
C 162
(3)
P 		59.5
(-3)
A 		70.2
(-2.4)
M 		65
(-2.7)
P 		103
avr 156.1
(3) 		52.3
(-2.8) 		48.1
(-3) 		60.2
(-2.3) 		108

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 113
1 2 3 4 5 6 range
A 58.1
(-2)
P 		112
(3)
P 		61.3
(-1.7)
P 		55
(-2.3)
P 		47.5
(-3)
P 		62.3
(-1.6)
P 		65
B 81.8
(-0.2)
P 		126.7
(3)
P 		59.8
(-1.8)
P 		43.7
(-3)
P 		82.6
(-0.2)
P 		59.8
(-1.8)
P 		83
C 141.9
(3)
P 		94.9
(0.5)
P 		67.6
(-0.9)
P 		28.6
(-3)
A 		61.7
(-1.2)
P 		45.6
(-2.1)
P 		113
avr 93.9
(1.5) 		111.2
(3) 		62.9
(-1.2) 		42.4
(-3) 		63.9
(-1.1) 		55.9
(-1.8) 		69
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191075_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15591
Sequence Type Exemplar sequence
Representative Public ID B0348.4 NCBI
Target Description B0348.4 /REP_DB=WormBase Gene ID /WP=CE23507 /CHR=5 /FEA=Sanger Annotation /DEF=locus:egl-8 phospholipase (ST.LOUIS) TR:O61956 protein_id:AAC17812.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026176(11), NM_001026175(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026176 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-8) (egl-8) mRNA, complete cds. 11/11 None
NM_001026175 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-8) (egl-8) mRNA, complete cds. 11/11 None
SNAP00000028743 ENSEMBL cdna:SNAP chromosome:CEL140:V:37863:48904:1 11/11 None
GENEFINDER00000028748 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:30660:47150:1 11/11 None
B0348.4a ENSEMBL cdna:known chromosome:CEL140:V:20653:43515:1 gene:B0348.4 11/11 A 
B0348.4b ENSEMBL cdna:known chromosome:CEL140:V:20653:43522:1 gene:B0348.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
178537 3 2 NM_001026175 175785_at (11), 191075_s_at (11)
NM_001026176 175785_at (11), 191075_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:20834-42998(+) 100.0 100.0
Public Domain and Genome References
Ensembl B0348.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL77168 Egg laying defective protein 8, isoform b [Caenorhabditis elegans] ref|NP_001021347.1| EGg Laying defective family member (egl-8) [Caenorhabditis elegans] gb|AAF05701.1| phospholipase C beta homolog EGL-8 [Caenorhabditis elegans] 0.0
blast AAL77167 Egg laying defective protein 8, isoform a [Caenorhabditis elegans] ref|NP_001021346.1| EGg Laying defective family member (egl-8) [Caenorhabditis elegans] 0.0
blast AAF01458 phospholipase C beta [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
scop a.2.5.Prefoldin All alpha proteins; Long alpha-hairpin; Prefoldin; Prefoldin 9.80000019073486
Pfam IPR000909 EMBL-EBI Phosphatidylinositol-specific phospholipase C, X domain 4.9E-77
Pfam IPR009535 EMBL-EBI Protein of unknown function DUF1154 1.4E-36
Pfam IPR001711 EMBL-EBI Phosphatidylinositol-specific phospholipase C, Y domain 3.2E-70
Pfam IPR000008 EMBL-EBI C2 domain 2.4E-18
Pfam IPR009535 EMBL-EBI Protein of unknown function DUF1154 2.1E-32
Pfam IPR009535 EMBL-EBI Protein of unknown function DUF1154 5.1E-32
Pfam IPR009535 EMBL-EBI Protein of unknown function DUF1154 1.4E-36
Pfam IPR001711 EMBL-EBI Phosphatidylinositol-specific phospholipase C, Y domain 3.2E-70
Pfam IPR000008 EMBL-EBI C2 domain 2.4E-18
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000028743 1 316-338
GENEFINDER00000028748 1 863-885
Sequence
Target Sequence 
>C. ELEGANS:191075_S_AT
ccttctggcgcgagttgcagtgggtactccactgggggtccttcgacaccggtggcatgt
aactctgatggtaccgggtctccagcgactattggaagtccagtgccacaagatttggtc
aataatgatagggtccgatctctcgtgaacactcaaaccggagaatggtccgctatggtc
cgtagacatgacgaggaagagtttgagctgaaaaaagtacagctcaaagagcaattcgat
ctgctaagaaaactcatgagtgaagctcaaaagaaccaaatgttggctctgaagctccgg
ttagaagccgaagggaaagatctaaagcagacgcagacgaaaaaaagcatggaagatgcg
aaagttattcaattggacaaaggtattaagaccaaggcagaacgtgatcgtcgtgtcaag
gaactcaacgagaagaacctgaaaatgtttgtggaggagaggaagagattagccatgaaa
gcccaaaagcacgaagagcaactcacaaaacgacatctcgaccagctcgagcaactagac
aaggatttccataaggccttggatgcggaggtcggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCTTCTGGCGCGAGTTGCAGTGGGT 442 269 3517 Antisense
GCAGTGGGTACTCCACTGGGGGTCC 438 309 3533 Antisense
GACACCGGTGGCATGTAACTCTGAT 507 367 3561 Antisense
TAACTCTGATGGTACCGGGTCTCCA 269 637 3576 Antisense
TAATGATAGGGTCCGATCTCTCGTG 134 633 3639 Antisense
GATCTCTCGTGAACACTCAAACCGG 389 419 3653 Antisense
ATGGTCCGCTATGGTCCGTAGACAT 16 51 3681 Antisense
AATGTTGGCTCTGAAGCTCCGGTTA 464 161 3795 Antisense
GGCAGAACGTGATCGTCGTGTCAAG 178 535 3912 Antisense
GACATCTCGACCAGCTCGAGCAACT 617 369 4028 Antisense
TAAGGCCTTGGATGCGGAGGTCGGC 105 635 4068 Antisense
Sequence Source Affymetrix Proprietary Database