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[191073_s_at]

Description F42G8.3 /REP_DB=WormBase Gene ID /WP=CE17062 /GEN=pmk-2 /TR=Q17445 /GB=AAB92064.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protein kinase
Cluster 6grp_highest_10_10 > 22      6grp_high1_10_10 > 02      4grp_high_8_8 > 17      6grp_highest_nonorm > 61      6grp_total > 24      4grp_total > 30      6grp_highest_sub > De      6grp_cgc > 20      6grp_high1_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 370
1 2 3 4 range
A 567
(3)
P 		288.5
(-2.4)
P 		259.7
(-3)
P 		370.2
(-0.8)
M 		307
B 587.2
(3)
P 		398.3
(-0.2)
P 		234
(-3)
P 		424
(0.2)
P 		353
C 604.3
(3)
P 		305.1
(-2.5)
P 		278.1
(-3)
P 		460.6
(0.4)
P 		326
avr 586.2
(3) 		330.6
(-1.7) 		257.3
(-3) 		418.3
(-0.1) 		329

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 278
1 2 3 4 5 6 range
A 506
(3)
P 		464.5
(1.9)
P 		417.1
(0.7)
P 		375.2
(-0.3)
P 		271.1
(-3)
P 		327.8
(-1.6)
P 		235
B 525.3
(2.4)
P 		459.5
(0.7)
P 		548.4
(3)
P 		357.2
(-1.9)
P 		318.1
(-2.9)
P 		315.7
(-3)
P 		233
C 549.3
(3)
P 		475.7
(1.3)
P 		413.7
(-0.1)
P 		445.1
(0.6)
P 		291
(-3)
P 		393.6
(-0.6)
P 		258
avr 526.9
(3) 		466.6
(1.5) 		459.7
(1.3) 		392.5
(-0.5) 		293.4
(-3) 		345.7
(-1.7) 		234
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 152
1 2 3 4 range
A 206.4
(3)
P 		62.6
(-2.7)
P 		67.9
(-2.5)
P 		54.2
(-3)
M 		152
B 187.3
(3)
P 		98.4
(-1)
P 		54.2
(-3)
P 		90.4
(-1.4)
P 		133
C 200.1
(3)
P 		87.7
(-2.5)
P 		96.9
(-2.1)
P 		77.7
(-3)
P 		122
avr 197.9
(3) 		82.9
(-2.5) 		73
(-3) 		74.1
(-2.9) 		125

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 124
1 2 3 4 5 6 range
A 93.3
(-2.1)
P 		165.2
(3)
P 		90.8
(-2.2)
P 		95.2
(-1.9)
P 		81.8
(-2.9)
P 		79.8
(-3)
P 		85
B 182.8
(3)
P 		152.9
(1)
P 		133.2
(-0.4)
P 		94.6
(-3)
P 		129.2
(-0.6)
P 		117.7
(-1.4)
P 		88
C 203.6
(3)
P 		145.2
(-0.1)
P 		105.3
(-2.2)
P 		89.5
(-3)
P 		94.2
(-2.8)
P 		131.8
(-0.8)
P 		114
avr 159.9
(3) 		154.4
(2.5) 		109.8
(-1.5) 		93.1
(-3) 		101.7
(-2.2) 		109.8
(-1.5) 		67
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 191073_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8817
Sequence Type Exemplar sequence
Representative Public ID F42G8.3 NCBI
Target Description F42G8.3 /REP_DB=WormBase Gene ID /WP=CE17062 /GEN=pmk-2 /TR=Q17445 /GB=AAB92064.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171913(11), NM_171392(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171913 NCBI Caenorhabditis elegans P38 Map Kinase family member (pmk-2) (pmk-2) mRNA, complete cds. 11/11 None
NM_171392 NCBI Caenorhabditis elegans P38 Map Kinase family member (pmk-2) (pmk-2) mRNA, complete cds. 11/11 None
SNAP00000005552 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8147034:8150722:1 10/11 None
GENEFINDER00000005564 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8146744:8151149:1 10/11 None
F42G8.3b ENSEMBL cdna:known chromosome:CEL140:IV:8143481:8148621:1 gene:F42G8.3 11/11 None
F42G8.3a ENSEMBL cdna:known chromosome:CEL140:IV:8143492:8148619:1 gene:F42G8.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8143497-8148318(+) 100.0 100.0
Public Domain and Genome References
Ensembl F42G8.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM98017 P38 map kinase family protein 2, isoform b [Caenorhabditis elegans] ref|NP_741458.2| P38 Map Kinase family member (pmk-2) [Caenorhabditis elegans] 0.0
blast AAM98016 P38 map kinase family protein 2, isoform a [Caenorhabditis elegans] ref|NP_741457.1| P38 Map Kinase family member (pmk-2) [Caenorhabditis elegans] sp|Q8MXI4|PMK2_CAEEL Mitogen-activated protein kinase pmk-2 (p38 MAP kinase 2) (Stress-activated protein kinase pmk-2) 0.0
blast T32643 hypothetical protein F42G8.3 - Caenorhabditis elegans 0.0
blast AAB00664 P38 map kinase family protein 1 [Caenorhabditis elegans] ref|NP_501365.1| P38 Map Kinase family member (pmk-1) [Caenorhabditis elegans] sp|Q17446|PMK1_CAEEL Mitogen-activated protein kinase pmk-1 (p38 MAP kinase 1) (Stress-activated protein kinase pmk-1) 0.0
blast CAE58424 Hypothetical protein CBG01555 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 6.0E-68
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-71
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-43
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-75
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-41
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-75
hanks 3.1.1 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; CDC2Hs 4.0E-69
hanks 3.1.1 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; CDC2Hs 1.0E-72
hanks 3.1.1 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; CDC2Hs 2.0E-76
hanks 3.1.1 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; CDC2Hs 1.0E-76
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 3.4E-79
Pfam IPR000719 EMBL-EBI Protein kinase 4.7E-76
Pfam IPR000719 EMBL-EBI Protein kinase 1.2E-49
Pfam IPR000719 EMBL-EBI Protein kinase 7.6E-84
Pfam IPR000719 EMBL-EBI Protein kinase 6.9E-46
Pfam IPR000719 EMBL-EBI Protein kinase 7.6E-84
Sequence
Target Sequence 
>C. ELEGANS:191073_S_AT
gctcgtgctcaagacgccgaaatgactggatacgtggcaacaagatggtacagagctcca
gagattatgctcaattggatgcattatacacagactgttgatgtttggtcagttggatgt
attcttgcggaactggtatccgggagaccattgtttccaggcgatgatcatattgatcag
ctaacaaagataatgtcagttgtgggtaccccaaaagaagaattctggtccaaaattcaa
tcagaagaagctcgaaattatattaaaaatcgttctccaattatccgtcaggacttcgtt
acattgtttccaatggcttctccatatgctctcgaacttctcgaaatgatgctaattttg
gatccggacagaagaatttctgtatcaagtgctcttcgtcatgactatcttcgtgaatac
tctgtgccaaatgatgaaccagttgcaatggatacggtaataaatagcatagtcacaatt
gatccagctgaagaacgagcaacaacgttatccgactggcgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCGTGCTCAAGACGCCGAAATGA 565 299 739 Antisense
TCCGGGAGACCATTGTTTCCAGGCG 227 595 877 Antisense
GATAATGTCAGTTGTGGGTACCCCA 516 413 927 Antisense
ATCGTTCTCCAATTATCCGTCAGGA 307 35 1007 Antisense
ATCCGTCAGGACTTCGTTACATTGT 377 37 1021 Antisense
GTTTCCAATGGCTTCTCCATATGCT 503 451 1044 Antisense
TCCATATGCTCTCGAACTTCTCGAA 412 591 1059 Antisense
ATTTCTGTATCAAGTGCTCTTCGTC 177 17 1114 Antisense
GCTCTTCGTCATGACTATCTTCGTG 305 301 1129 Antisense
ATCTTCGTGAATACTCTGTGCCAAA 390 31 1145 Antisense
GCAACAACGTTATCCGACTGGCGAG 382 317 1237 Antisense
Sequence Source Affymetrix Proprietary Database