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[191064_at] DnaJ domain ; Thioredoxin

Description Y47H9C.5 /REP_DB=WormBase Gene ID /WP=CE20265 /TR=Q9XWE1 /GB=CAA21734.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=DnaJ domain ; Thioredoxin
Cluster 6grp_highest_10_10 > 11      6grp_high1_10_10 > 01      6grp_highest_nonorm > 80      6grp_total > 23      4grp_total > 51      6grp_cgc > 20      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 305
1 2 3 4 range
A 178.8
(-0.8)
P 		312
(3)
P 		176.1
(-0.9)
P 		103.4
(-3)
A 		209
B 155.9
(0.6)
P 		212
(2.2)
P 		240.7
(3)
P 		26.4
(-3)
A 		214
C 135.4
(-0.9)
P 		331.8
(3)
P 		228.4
(0.9)
P 		33.1
(-3)
A 		299
avr 156.7
(-0.3) 		285.3
(3) 		215.1
(1.2) 		54.3
(-3) 		231

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 272
1 2 3 4 5 6 range
A 289.6
(3)
P 		218
(-0.3)
P 		245.6
(1)
P 		280
(2.6)
P 		187.8
(-1.7)
P 		159.2
(-3)
P 		130
B 409.2
(3)
P 		252.5
(-0.5)
P 		284.8
(0.3)
P 		243.2
(-0.7)
P 		137.3
(-3)
P 		160
(-2.5)
P 		272
C 322.6
(3)
P 		287.6
(1.8)
P 		256.8
(0.7)
P 		277.1
(1.4)
P 		158.6
(-2.8)
P 		152.5
(-3)
P 		170
avr 340.5
(3) 		252.7
(0.1) 		262.4
(0.4) 		266.8
(0.6) 		161.2
(-2.9) 		157.2
(-3) 		183
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 90
1 2 3 4 range
A 65.1
(2.7)
P 		67.7
(3)
P 		46
(0.5)
P 		15.1
(-3)
A 		53
B 49.7
(2.3)
P 		52.4
(2.6)
P 		55.8
(3)
P 		5.6
(-3)
A 		50
C 44.8
(-0.4)
P 		95.4
(3)
P 		79.6
(1.9)
P 		5.6
(-3)
A 		90
avr 53.2
(1.2) 		71.8
(3) 		60.5
(1.9) 		8.8
(-3) 		63

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 104
1 2 3 4 5 6 range
A 53.4
(-0.7)
P 		77.5
(3)
P 		53.5
(-0.7)
P 		71
(2)
P 		56.7
(-0.2)
P 		38.8
(-3)
P 		39
B 142.4
(3)
P 		84.1
(-1)
P 		69.2
(-2.1)
P 		64.4
(-2.4)
P 		55.8
(-3)
P 		59.7
(-2.7)
P 		87
C 119.6
(3)
P 		87.8
(0.2)
P 		65.3
(-1.8)
P 		55.7
(-2.6)
P 		51.3
(-3)
P 		51.1
(-3)
P 		69
avr 105.1
(3) 		83.1
(0.6) 		62.7
(-1.6) 		63.7
(-1.5) 		54.6
(-2.5) 		49.9
(-3) 		55
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191064_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.876
Sequence Type Exemplar sequence
Representative Public ID Y47H9C.5 NCBI
Target Description Y47H9C.5 /REP_DB=WormBase Gene ID /WP=CE20265 /TR=Q9XWE1 /GB=CAA21734.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=DnaJ domain ; Thioredoxin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060609(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060609 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-27) (dnj-27) mRNA, complete cds. 11/11 A 
GENEFINDER00000036892 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:11885928:11895269:1 11/11 None
SNAP00000036883 ENSEMBL cdna:SNAP chromosome:CEL140:I:11892255:11893684:1 10/11 None
Y47H9C.5.2 ENSEMBL cdna:known chromosome:CEL140:I:11885923:11895348:1 gene:Y47H9C.5 11/11 None
Y47H9C.5.1 ENSEMBL cdna:known chromosome:CEL140:I:11885925:11895390:1 gene:Y47H9C.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:11885858-11895200(+) 100.0 100.0
Public Domain and Genome References
Gene Title DnaJ domain ; Thioredoxin
Gene Symbol dnj-27
Ensembl Y47H9C.5
Entrez Gene 173065 Entrez gene
SwissProt Q9XWE1 EMBL-EBI
WormBase CE20265 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604799_AT similar to DnaJ (Hsp40) homolog, subfamily C, member 10 cfa
CANINE_2:CFA.18577.1.S1_S_AT similar to DnaJ (Hsp40) homolog, subfamily C, member 10 cfa
CANINE_2:CFA.1884.1.S1_AT similar to DnaJ (Hsp40) homolog, subfamily C, member 10 cfa
CANINE_2:CFAAFFX.22084.1.S1_S_AT similar to DnaJ (Hsp40) homolog, subfamily C, member 10 cfa
CHICKEN:GGA.15950.1.S1_AT DnaJ (Hsp40) homolog, subfamily C, member 10 gga
CHICKEN:GGAAFFX.5560.1.S1_S_AT DnaJ (Hsp40) homolog, subfamily C, member 10 gga
HG-U133_PLUS_2:229588_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HG-U133B:229588_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HG-U95C:48849_I_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HU35KSUBA:RC_AA243695_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HU35KSUBC:RC_W42788_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HU35KSUBC:RC_AA453624_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HU35KSUBD:RC_AA600176_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HU35KSUBD:RC_AA455178_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
U133_X3P:HS.69165.0.A1_3P_X_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
U133_X3P:HS.69165.0.A1_3P_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
U133_X3P:HS.1098.0.A2_3P_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
U133_X3P:HS.1098.0.A1_3P_S_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
U133_X3P:HS.1098.0.A1_3P_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HG-U133A_2:221781_S_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HG-U133_PLUS_2:221781_S_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HG-U133A:221781_S_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HG-U133A_2:221782_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HG-U133_PLUS_2:221782_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HG-U133A:221782_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HG-U133_PLUS_2:225174_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HG-U133B:225174_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HG-U95C:48850_R_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HG-U95B:51927_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HG-U95B:52343_S_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HG-U95C:54808_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HG-U95B:58797_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
HU35KSUBB:RC_AA100967_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
U133_X3P:229588_3P_AT DnaJ (Hsp40) homolog, subfamily C, member 10 hs
MOE430A:1452230_AT DnaJ (Hsp40) homolog, subfamily C, member 10 mm
MOUSE430A_2:1452230_AT DnaJ (Hsp40) homolog, subfamily C, member 10 mm
MOUSE430_2:1452230_AT DnaJ (Hsp40) homolog, subfamily C, member 10 mm
MOE430A:1426904_S_AT DnaJ (Hsp40) homolog, subfamily C, member 10 mm
MOUSE430A_2:1426904_S_AT DnaJ (Hsp40) homolog, subfamily C, member 10 mm
MOUSE430_2:1426904_S_AT DnaJ (Hsp40) homolog, subfamily C, member 10 mm
MU11KSUBA:AA189984_S_AT DnaJ (Hsp40) homolog, subfamily C, member 10, mRNA (cDNA clone MGC:28281 IMAGE:4010341) mm
MG-U74AV2:160144_AT DnaJ (Hsp40) homolog, subfamily C, member 10, mRNA (cDNA clone MGC:28281 IMAGE:4010341) mm
MG-U74BV2:164344_F_AT DnaJ (Hsp40) homolog, subfamily C, member 10, mRNA (cDNA clone MGC:28281 IMAGE:4010341) mm
MOE430A:1426905_A_AT DnaJ (Hsp40) homolog, subfamily C, member 10, mRNA (cDNA clone MGC:28281 IMAGE:4010341) mm
MOUSE430A_2:1426905_A_AT DnaJ (Hsp40) homolog, subfamily C, member 10, mRNA (cDNA clone MGC:28281 IMAGE:4010341) mm
MOUSE430_2:1426905_A_AT DnaJ (Hsp40) homolog, subfamily C, member 10, mRNA (cDNA clone MGC:28281 IMAGE:4010341) mm
MU19KSUBA:TC23299_AT DnaJ (Hsp40) homolog, subfamily C, member 10, mRNA (cDNA clone MGC:28281 IMAGE:4010341) mm
MU19KSUBB:TC32021_AT DnaJ (Hsp40) homolog, subfamily C, member 10, mRNA (cDNA clone MGC:28281 IMAGE:4010341) mm
RG-U34B:RC_AI010958_AT DnaJ (Hsp40) homolog, subfamily C, member 10 (predicted) rn
RG-U34C:RC_AI172034_AT DnaJ (Hsp40) homolog, subfamily C, member 10 (predicted) rn
RAE230A:1389290_AT DnaJ (Hsp40) homolog, subfamily C, member 10 (predicted) rn
RAT230_2:1389290_AT DnaJ (Hsp40) homolog, subfamily C, member 10 (predicted) rn
RAE230A:1373813_AT DnaJ (Hsp40) homolog, subfamily C, member 10 (predicted) rn
RAT230_2:1373813_AT DnaJ (Hsp40) homolog, subfamily C, member 10 (predicted) rn
RAE230B:1394634_AT DnaJ (Hsp40) homolog, subfamily C, member 10 (predicted) rn
RAT230_2:1394634_AT DnaJ (Hsp40) homolog, subfamily C, member 10 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
6457 protein folding inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
20037 heme binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA21734 Hypothetical protein Y47H9C.5 [Caenorhabditis elegans] ref|NP_493010.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-27) [Caenorhabditis elegans] 0.0
blast CAE69614 Hypothetical protein CBG15846 [Caenorhabditis briggsae] 0.0
blast CAA21734 Hypothetical protein Y47H9C.5 [Caenorhabditis elegans] ref|NP_493010.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-27) [Caenorhabditis elegans] 1.0E-171
blast AAH70632 MGC81459 protein [Xenopus laevis] 1.0E-165
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 2.0000000544904E-27
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 3.2E-32
Pfam IPR006662 EMBL-EBI Thioredoxin type domain 4.0E-14
Pfam IPR006662 EMBL-EBI Thioredoxin type domain 1.3E-7
Pfam IPR006662 EMBL-EBI Thioredoxin type domain 1.8E-17
Pfam IPR006662 EMBL-EBI Thioredoxin type domain 7.1E-26
Pfam IPR006662 EMBL-EBI Thioredoxin type domain 1.3E-7
Pfam IPR006662 EMBL-EBI Thioredoxin type domain 1.8E-17
Sequence
Target Sequence 
>C. ELEGANS:191064_AT
tggtgtggaccttgccagcaattggctccggagctacagaaagccgccaggcaaattgcg
gcgttcgatgaaaatgcacatgttgcatcaattgattgtcagaaatatgcgcaattttgc
acgaatactcagattaacagctacccgactgttaggatgtaccctgccaagaagactaag
cagccgaggagatctccgttttacgactac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGTGTGGACCTTGCCAGCAATTGG 117 551 1750 Antisense
TGCCAGCAATTGGCTCCGGAGCTAC 70 585 1762 Antisense
CCGGAGCTACAGAAAGCCGCCAGGC 597 263 1777 Antisense
CCGCCAGGCAAATTGCGGCGTTCGA 417 259 1793 Antisense
GCGGCGTTCGATGAAAATGCACATG 495 291 1807 Antisense
GCGCAATTTTGCACGAATACTCAGA 217 289 1858 Antisense
ATACTCAGATTAACAGCTACCCGAC 185 23 1874 Antisense
GCTACCCGACTGTTAGGATGTACCC 312 301 1889 Antisense
GGATGTACCCTGCCAAGAAGACTAA 123 515 1904 Antisense
GACTAAGCAGCCGAGGAGATCTCCG 462 373 1923 Antisense
GAGGAGATCTCCGTTTTACGACTAC 640 403 1935 Antisense
Sequence Source Affymetrix Proprietary Database