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[191040_at]

Description B0280.1 /REP_DB=WormBase Gene ID /WP=CE24762 /TR=SW:P41992 /GB=AAA19080.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=prenyltransferase BET2
Cluster 6grp_highest_10_10 > 57      6grp_high1_10_10 > 75      4grp_highest_8_8 > 04      4grp_high_8_8 > 37      6grp_highest_nonorm > 85      6grp_total > 75      4grp_total > 42      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 203
1 2 3 4 range
A 635.4
(-0.7)
P 		627.1
(-1.2)
P 		595.7
(-3)
P 		697.9
(3)
P 		102
B 708.6
(1.2)
P 		745
(3)
P 		621.9
(-3)
P 		645.8
(-1.8)
P 		123
C 627
(-3)
P 		686.9
(-0.9)
P 		798.8
(3)
P 		677.1
(-1.3)
P 		172
avr 657
(-3) 		686.3
(3) 		672.1
(0.1) 		673.6
(0.4) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 467
1 2 3 4 5 6 range
A 688.7
(-2.8)
P 		679.6
(-3)
P 		807.8
(-0.6)
P 		996.1
(3)
P 		696.1
(-2.7)
P 		862.5
(0.5)
P 		317
B 630
(-2.8)
P 		624.4
(-3)
P 		677.1
(-1.6)
P 		702.1
(-0.9)
P 		645.9
(-2.4)
P 		846.3
(3)
P 		222
C 789.3
(0.4)
P 		528.9
(-3)
P 		760.8
(-0)
P 		742.6
(-0.2)
P 		642.6
(-1.5)
P 		994.8
(3)
P 		466
avr 702.7
(-1.1) 		611
(-3) 		748.6
(-0.2) 		813.6
(1.2) 		661.5
(-2) 		901.2
(3) 		290
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 176
1 2 3 4 range
A 231.3
(3)
P 		136.1
(-1.4)
P 		155.7
(-0.5)
P 		102.2
(-3)
P 		129
B 226
(3)
P 		184
(0.1)
P 		144.1
(-2.6)
P 		137.6
(-3)
P 		88
C 207.6
(0.4)
P 		197.5
(0)
P 		278.4
(3)
P 		114.2
(-3)
P 		164
avr 221.6
(3) 		172.5
(0.2) 		192.7
(1.3) 		118
(-3) 		104

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 206
1 2 3 4 5 6 range
A 127
(-3)
P 		241.7
(2.5)
P 		175.9
(-0.7)
P 		252.6
(3)
P 		210.1
(1)
P 		210
(1)
P 		126
B 219.2
(-0.8)
P 		207.8
(-1.3)
P 		164.4
(-3)
P 		186
(-2.1)
P 		262.4
(0.9)
P 		315.6
(3)
P 		151
C 292.6
(1.7)
P 		161.4
(-2.6)
P 		193.6
(-1.6)
P 		149.3
(-3)
P 		207.9
(-1.1)
P 		333
(3)
P 		184
avr 212.9
(-1.1) 		203.6
(-1.6) 		178
(-3) 		196
(-2) 		226.8
(-0.3) 		286.2
(3) 		108
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191040_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7360
Sequence Type Exemplar sequence
Representative Public ID B0280.1 NCBI
Target Description B0280.1 /REP_DB=WormBase Gene ID /WP=CE24762 /TR=SW:P41992 /GB=AAA19080.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=prenyltransferase BET2
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066158(11), NM_171183(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066158 NCBI Caenorhabditis elegans B0280.1a (B0280.1) mRNA, complete cds. 11/11 A 
NM_171183 NCBI Caenorhabditis elegans B0280.1b (B0280.1) mRNA, complete cds. 11/11 None
GENEFINDER00000041625 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7133763:7134930:1 10/11 None
SNAP00000041615 ENSEMBL cdna:SNAP chromosome:CEL140:III:7134046:7136332:1 11/11 None
B0280.1a ENSEMBL cdna:known chromosome:CEL140:III:7133737:7136332:1 gene:B0280.1 11/11 A 
B0280.1b ENSEMBL cdna:known chromosome:CEL140:III:7133807:7136356:1 gene:B0280.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7133853-7136330(+) 99.5 99.8
Public Domain and Genome References
Ensembl B0280.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL50321 Hypothetical protein B0280.1a [Caenorhabditis elegans] ref|NP_498559.1| B0280.1a [Caenorhabditis elegans] sp|P41992|PFTB_CAEEL Probable protein farnesyltransferase beta subunit (CAAX farnesyltransferase beta subunit) (RAS proteins prenyltransferase beta) (FTase-beta) 0.0
blast CAE70140 Hypothetical protein CBG16602 [Caenorhabditis briggsae] 1.0E-177
blast AAL50322 Hypothetical protein B0280.1b [Caenorhabditis elegans] ref|NP_741214.1| B0280.1b [Caenorhabditis elegans] 1.0E-177
blast AAL50322 Hypothetical protein B0280.1b [Caenorhabditis elegans] ref|NP_741214.1| B0280.1b [Caenorhabditis elegans] 1.0E-173
blast T15296 hypothetical protein B0280.1 - Caenorhabditis elegans 1.0E-173
blast AAL50321 Hypothetical protein B0280.1a [Caenorhabditis elegans] ref|NP_498559.1| B0280.1a [Caenorhabditis elegans] sp|P41992|PFTB_CAEEL Probable protein farnesyltransferase beta subunit (CAAX farnesyltransferase beta subunit) (RAS proteins prenyltransferase beta) (FTase-beta) 1.0E-173
Protein Domains
Database ID Description E-Value
Pfam IPR001330 EMBL-EBI Prenyltransferase/squalene oxidase 1.2E-14
Pfam IPR001330 EMBL-EBI Prenyltransferase/squalene oxidase 3.1E-13
Pfam IPR001330 EMBL-EBI Prenyltransferase/squalene oxidase 1.0E-17
Pfam IPR001330 EMBL-EBI Prenyltransferase/squalene oxidase 3.4E-17
Pfam IPR001330 EMBL-EBI Prenyltransferase/squalene oxidase 6.4E-15
Pfam IPR001330 EMBL-EBI Prenyltransferase/squalene oxidase 7.1E-6
Pfam IPR001330 EMBL-EBI Prenyltransferase/squalene oxidase 1.2E-14
Pfam IPR001330 EMBL-EBI Prenyltransferase/squalene oxidase 3.1E-13
Pfam IPR001330 EMBL-EBI Prenyltransferase/squalene oxidase 1.0E-17
Pfam IPR001330 EMBL-EBI Prenyltransferase/squalene oxidase 3.4E-17
Pfam IPR001330 EMBL-EBI Prenyltransferase/squalene oxidase 2.4E-5
Pfam IPR001330 EMBL-EBI Prenyltransferase/squalene oxidase 1.2E-14
Pfam IPR001330 EMBL-EBI Prenyltransferase/squalene oxidase 3.1E-13
Pfam IPR001330 EMBL-EBI Prenyltransferase/squalene oxidase 1.0E-17
Pfam IPR001330 EMBL-EBI Prenyltransferase/squalene oxidase 3.4E-17
Pfam IPR001330 EMBL-EBI Prenyltransferase/squalene oxidase 6.4E-15
Sequence
Target Sequence 
>C. ELEGANS:191040_AT
gatacacgattcactctgtgctctctcgctacttgtcatttacttggtcgactgtccaca
ttgaatatcgattcagctgtacgatttctcatgagatgttacaatacggatggtggattc
ggaacacgcccaggatctgaaagtcattccggacagatatactgttgtgttggagctctg
gcgattgccggacgacttgatgagattgatagagatagaacagccgaatggcttgctttc
aggcaatgtgatagtggaggattaaatggtcggcctgagaaattacccgatgtatgttat
tcctggtgggttctcgcttctctcgccattctcggacgcctcaatttcattgattctgat
gctatgaaaaagttcatatacgcttgtcaagatgatgaaactggaggatttgcagacagg
cccggagattgtgcggatccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATACACGATTCACTCTGTGCTCTC 623 423 454 Antisense
CTCTCGCTACTTGTCATTTACTTGG 51 231 476 Antisense
TTACTTGGTCGACTGTCCACATTGA 657 683 493 Antisense
CGATTCAGCTGTACGATTTCTCATG 123 249 522 Antisense
TTCGGAACACGCCCAGGATCTGAAA 454 693 571 Antisense
TTGTGTTGGAGCTCTGGCGATTGCC 290 707 618 Antisense
TGGCGATTGCCGGACGACTTGATGA 480 545 632 Antisense
AGCCGAATGGCTTGCTTTCAGGCAA 157 83 675 Antisense
TCTCGGACGCCTCAATTTCATTGAT 561 611 783 Antisense
TGGAGGATTTGCAGACAGGCCCGGA 493 549 855 Antisense
CAGGCCCGGAGATTGTGCGGATCCC 424 209 870 Antisense
Sequence Source Affymetrix Proprietary Database