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[191005_at]

Description W02B12.12 /REP_DB=WormBase Gene ID /WP=CE21239 /TR=Q23128 /GB=CAA91402.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_total > 98      4grp_total > 24      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 1.1
(-3)
A 		4.2
(1)
A 		3.7
(0.4)
A 		5.7
(3)
A 		5
B 5.8
(-1.9)
A 		4.9
(-3)
A 		9.7
(3)
A 		7.4
(0.1)
A 		5
C 4
(-3)
A 		5.2
(-1.8)
A 		7.4
(0.4)
A 		10
(3)
A 		6
avr 3.6
(-3) 		4.8
(-1.2) 		6.9
(1.8) 		7.7
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 296
1 2 3 4 5 6 range
A 4.6
(-3)
A 		12.5
(-2.8)
A 		4.5
(-3)
A 		54
(-2)
A 		181.6
(0.6)
P 		298.3
(3)
P 		294
B 5.6
(-2.9)
A 		6
(-2.9)
A 		2
(-3)
A 		8.1
(-2.9)
A 		166.7
(0.7)
P 		267.5
(3)
P 		266
C 4
(-3)
A 		23.2
(-2.4)
A 		3.7
(-3)
A 		55.9
(-1.5)
A 		186.7
(2.2)
P 		213.6
(3)
P 		210
avr 4.7
(-3) 		13.9
(-2.8) 		3.4
(-3) 		39.3
(-2.2) 		178.3
(1.1) 		259.8
(3) 		256
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2
1 2 3 4 range
A 0.4
(-3)
A 		0.9
(2)
A 		1
(3)
A 		0.8
(1)
A 		1
B 1.8
(0.3)
A 		1.2
(-3)
A 		2.3
(3)
A 		1.6
(-0.8)
A 		1
C 1.3
(-3)
A 		1.5
(-2.1)
A 		2.6
(3)
A 		1.7
(-1.2)
A 		1
avr 1.2
(-3) 		1.2
(-3) 		2
(3) 		1.4
(-1.5) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 99
1 2 3 4 5 6 range
A 0.9
(-3)
A 		4.5
(-2.7)
A 		1
(-3)
A 		13.7
(-1.9)
A 		54.8
(1.5)
P 		72.6
(3)
P 		72
B 1.9
(-2.9)
A 		2
(-2.9)
A 		0.5
(-3)
A 		2.1
(-2.9)
A 		67.7
(1.1)
P 		99.8
(3)
P 		99
C 1.5
(-2.9)
A 		7.1
(-2.5)
A 		0.9
(-3)
A 		11.2
(-2.1)
A 		60.4
(2.1)
P 		71.5
(3)
P 		71
avr 1.4
(-3) 		4.5
(-2.7) 		0.8
(-3) 		9
(-2.4) 		61
(1.5) 		81.3
(3) 		81
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 191005_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3417
Sequence Type Exemplar sequence
Representative Public ID W02B12.12 NCBI
Target Description W02B12.12 /REP_DB=WormBase Gene ID /WP=CE21239 /TR=Q23128 /GB=CAA91402.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027215(11), NM_001027214(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027215 NCBI Caenorhabditis elegans W02B12.12b (W02B12.12) mRNA, complete cds. 11/11 None
NM_001027214 NCBI Caenorhabditis elegans W02B12.12a (W02B12.12) mRNA, complete cds. 11/11 None
W02B12.12b ENSEMBL cdna:known chromosome:CEL140:II:11476732:11479066:-1 gene:W02B12.12 11/11 A 
W02B12.12a ENSEMBL cdna:known chromosome:CEL140:II:11476787:11479066:-1 gene:W02B12.12 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11476783-11479063(-) 100.0 100.0
Public Domain and Genome References
Ensembl W02B12.12
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA91402 Hypothetical protein W02B12.12a [Caenorhabditis elegans] ref|NP_001022385.1| W02B12.12a [Caenorhabditis elegans] 0.0
blast CAH10788 Hypothetical protein W02B12.12b [Caenorhabditis elegans] ref|NP_001022386.1| W02B12.12b [Caenorhabditis elegans] 0.0
blast CAE59495 Hypothetical protein CBG02880 [Caenorhabditis briggsae] 1.0E-145
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-18
hanks 2.2.12 CaMK Group; CaMK II KIN1/SNF1/Nim1; YCL24 2.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 2.4E-58
Sequence
Target Sequence 
>C. ELEGANS:191005_AT
ggcacagagtatgatcttcctgacgatggagtacggtagtcaaggagacttgttacgcta
cgtccaagaacatggaggaattcacgagcaaaaagccggactatttatgtcccaactgat
acgtgggctccaattcatgcactcgataaatatcgcacaccgtgacatcaaattggagaa
catcattctattcgataattgcgtcaaactatccgatttcggatttgttcgtaaaatgga
tgagagcgcattgtcgtcgacattctgcggatcgaaatcctactcggcacctgagctgct
ccgtggaatcgtctacaacccatttttgtcggatgtctggtcgcttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCACAGAGTATGATCTTCCTGACG 338 535 498 Antisense
AGGAGACTTGTTACGCTACGTCCAA 612 53 540 Antisense
GACTATTTATGTCCCAACTGATACG 543 371 596 Antisense
GATACGTGGGCTCCAATTCATGCAC 103 425 615 Antisense
TAAATATCGCACACCGTGACATCAA 8 643 644 Antisense
AATTGCGTCAAACTATCCGATTTCG 193 179 694 Antisense
GGATGAGAGCGCATTGTCGTCGACA 53 515 735 Antisense
TGTCGTCGACATTCTGCGGATCGAA 628 557 749 Antisense
CACCTGAGCTGCTCCGTGGAATCGT 588 199 785 Antisense
GAATCGTCTACAACCCATTTTTGTC 229 329 803 Antisense
TTTTTGTCGGATGTCTGGTCGCTTG 184 675 820 Antisense
Sequence Source Affymetrix Proprietary Database