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[190976_s_at] WD domain, G-beta repeat

Description K02B12.3 /REP_DB=WormBase Gene ID /WP=CE06064 /TR=Q21115 /GB=CAB00033.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=WD domain, G-beta repeat
Cluster 6grp_highest_10_10 > 74      6grp_high1_10_10 > 37      4grp_highest_8_8 > 26      4grp_high_8_8 > 59      6grp_highest_nonorm > 75      6grp_total > 08      4grp_total > 60      6grp_highest_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 486
1 2 3 4 range
A 923.7
(0.1)
P 		1007.3
(1.4)
P 		1119.7
(3)
P 		708.6
(-3)
P 		411
B 1189.7
(3)
P 		942.9
(-2.9)
P 		1133.1
(1.6)
P 		939.3
(-3)
P 		250
C 948.1
(1.9)
P 		978.1
(2.5)
P 		1003.8
(3)
P 		703.6
(-3)
P 		300
avr 1020.5
(1.7) 		976.1
(0.8) 		1085.5
(3) 		783.8
(-3) 		302

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 385
1 2 3 4 5 6 range
A 922
(0.4)
P 		830.8
(-2)
P 		794.2
(-3)
P 		1022.9
(3)
P 		873.7
(-0.9)
P 		946
(1)
P 		229
B 1065.8
(3)
P 		770.7
(-2.5)
P 		755.1
(-2.8)
P 		874.9
(-0.5)
P 		741.8
(-3)
P 		921.5
(0.3)
P 		324
C 987.7
(0.8)
P 		754.9
(-3)
P 		1126.6
(3)
P 		877
(-1)
P 		824.1
(-1.9)
P 		861.2
(-1.3)
P 		372
avr 991.8
(3) 		785.5
(-3) 		892
(0.1) 		924.9
(1.1) 		813.2
(-2.2) 		909.6
(0.6) 		206
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 276
1 2 3 4 range
A 336.3
(3)
P 		218.7
(-0)
P 		292.7
(1.9)
P 		103.7
(-3)
P 		233
B 379.4
(3)
P 		232.9
(-1.9)
P 		262.6
(-0.9)
P 		200.2
(-3)
P 		179
C 313.9
(2.1)
P 		281.2
(1.2)
P 		349.8
(3)
P 		118.6
(-3)
P 		231
avr 343.2
(3) 		244.3
(0.1) 		301.7
(1.8) 		140.8
(-3) 		202

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 201
1 2 3 4 5 6 range
A 170
(-3)
P 		295.5
(3)
P 		173
(-2.9)
P 		259.4
(1.3)
P 		263.6
(1.5)
P 		230.3
(-0.1)
P 		126
B 370.9
(3)
P 		256.5
(-0.7)
P 		183.4
(-3)
P 		231.7
(-1.5)
P 		301.4
(0.8)
P 		343.6
(2.1)
P 		188
C 366.1
(3)
P 		230.4
(-1.3)
P 		286.7
(0.5)
P 		176.4
(-3)
P 		266.7
(-0.1)
P 		288.3
(0.5)
P 		190
avr 302.3
(3) 		260.8
(0.2) 		214.4
(-3) 		222.5
(-2.4) 		277.2
(1.3) 		287.4
(2) 		88
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190976_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1420
Sequence Type Exemplar sequence
Representative Public ID K02B12.3 NCBI
Target Description K02B12.3 /REP_DB=WormBase Gene ID /WP=CE06064 /TR=Q21115 /GB=CAB00033.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=WD domain, G-beta repeat
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059904(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059904 NCBI Caenorhabditis elegans K02B12.3 (K02B12.3) mRNA, complete cds. 11/11 None
SNAP00000001142 ENSEMBL cdna:SNAP chromosome:CEL140:I:8517388:8519440:-1 10/11 None
GENEFINDER00000001154 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8517388:8519440:-1 11/11 None
K02B12.3.1 ENSEMBL cdna:known chromosome:CEL140:I:8517252:8519440:-1 gene:K02B12.3 11/11 None
K02B12.3.2 ENSEMBL cdna:known chromosome:CEL140:I:8517311:8519537:-1 gene:K02B12.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8517318-8519371(-) 100.0 100.0
Public Domain and Genome References
Gene Title WD domain, G-beta repeat
Ensembl K02B12.3
Entrez Gene 172641 Entrez gene
SwissProt Q21115 EMBL-EBI
WormBase CE32461 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.7961.1.S1_AT similar to Prolactin regulatory element-binding protein (Mammalian guanine nucleotide exchange factor mSec12) cfa
DROSGENOME1:152142_AT dm
DROSOPHILA_2:1628795_S_AT dm
CHICKEN:GGAAFFX.6086.1.S1_AT similar to SEC12 gga
HG-U133A_2:217861_S_AT prolactin regulatory element binding hs
HG-U133A:217861_S_AT prolactin regulatory element binding hs
HG-U133_PLUS_2:217861_S_AT prolactin regulatory element binding hs
HG-FOCUS:217861_S_AT prolactin regulatory element binding hs
HU35KSUBA:RC_AA088263_AT prolactin regulatory element binding hs
HU35KSUBD:RC_R37205_S_AT prolactin regulatory element binding hs
U133_X3P:G7019502_3P_S_AT prolactin regulatory element binding hs
HG-U95B:57100_AT prolactin regulatory element binding hs
HG-U95E:87424_AT Prolactin regulatory element binding hs
MG-U74AV2:160087_AT prolactin regulatory element binding mm
MOUSE430A_2:1456037_X_AT prolactin regulatory element binding mm
MOE430A:1456037_X_AT prolactin regulatory element binding mm
MOUSE430_2:1456037_X_AT prolactin regulatory element binding mm
MU11KSUBA:AA102918_S_AT prolactin regulatory element binding mm
MG-U74AV2:161430_AT prolactin regulatory element binding mm
MOE430A:1416502_A_AT prolactin regulatory element binding mm
MOUSE430A_2:1416502_A_AT prolactin regulatory element binding mm
MOUSE430_2:1416502_A_AT prolactin regulatory element binding mm
MOUSE430_2:1420045_AT Prolactin regulatory element binding, mRNA (cDNA clone MGC:27689 IMAGE:4921119) mm
MOE430A:1420045_AT Prolactin regulatory element binding, mRNA (cDNA clone MGC:27689 IMAGE:4921119) mm
MOUSE430A_2:1420045_AT Prolactin regulatory element binding, mRNA (cDNA clone MGC:27689 IMAGE:4921119) mm
MOUSE430_2:1449717_AT Prolactin regulatory element binding, mRNA (cDNA clone MGC:27689 IMAGE:4921119) mm
MOE430A:1449717_AT Prolactin regulatory element binding, mRNA (cDNA clone MGC:27689 IMAGE:4921119) mm
MOUSE430A_2:1449717_AT Prolactin regulatory element binding, mRNA (cDNA clone MGC:27689 IMAGE:4921119) mm
MU19KSUBA:TC24673_AT Prolactin regulatory element binding, mRNA (cDNA clone MGC:27689 IMAGE:4921119) mm
MU19KSUBB:TC29787_AT Prolactin regulatory element binding, mRNA (cDNA clone MGC:27689 IMAGE:4921119) mm
MU19KSUBC:TC39412_AT Prolactin regulatory element binding, mRNA (cDNA clone MGC:27689 IMAGE:4921119) mm
MU19KSUBC:TC41078_AT Prolactin regulatory element binding, mRNA (cDNA clone MGC:27689 IMAGE:4921119) mm
Protein Similarities
Method ID Description E-Value
blast CAB00033 Hypothetical protein K02B12.3 [Caenorhabditis elegans] ref|NP_492305.2| K02B12.3 [Caenorhabditis elegans] 0.0
blast CAE67116 Hypothetical protein CBG12530 [Caenorhabditis briggsae] 1.0E-170
blast CAE67116 Hypothetical protein CBG12530 [Caenorhabditis briggsae] 1.0E-155
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 7.4E-7
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0034
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.096
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 7.4E-7
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.0034
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.2
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492305.2 1 402-424
K02B12.3.1 1 402-424
K02B12.3.2 1 402-424
SNAP00000001142 1 392-414
GENEFINDER00000001154 1 402-424
Sequence
Target Sequence 
>C. ELEGANS:190976_S_AT
agacgctcagccaattcttactattcaagctcataaagcagatgttgatgatatagactt
ttcaaaagattcgaagacaataatctctgttggagccgaaggtgcattcatttggagcac
acaaactggagcacgacttcttgatttacaattcccaattgaaatttcgcgtggattcaa
gagtatttcgtcactggcagtctcagattgcggaaatttcactgctgttggtacaatgtc
tggaagtgttctcgtgttcgatacccatgaatgtcgccgtctttacttctctcccgagtc
tcatggtctttttgtaacgggaattgaatttgtatcgaggacatcaccgtcaatttgcga
agatattcaatcagaaacacctggaatcgcatccggatttcaatctgccgttgtcacgct
ggccgctgacaagacaatgcaacttcatcgagtaccgtatcctcaacctcaaccttttag
tga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGACGCTCAGCCAATTCTTACTATT 450 71 558 Antisense
ACTGGAGCACGACTTCTTGATTTAC 406 97 682 Antisense
GAGTATTTCGTCACTGGCAGTCTCA 617 397 738 Antisense
GAAGTGTTCTCGTGTTCGATACCCA 24 335 800 Antisense
TCGATACCCATGAATGTCGCCGTCT 242 603 815 Antisense
CCCGAGTCTCATGGTCTTTTTGTAA 237 259 850 Antisense
GAAACACCTGGAATCGCATCCGGAT 47 357 931 Antisense
ATCCGGATTTCAATCTGCCGTTGTC 93 39 948 Antisense
CGCTGGCCGCTGACAAGACAATGCA 432 253 974 Antisense
GCAACTTCATCGAGTACCGTATCCT 683 317 996 Antisense
ATCCTCAACCTCAACCTTTTAGTGA 665 37 1016 Antisense
Sequence Source Affymetrix Proprietary Database