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[190939_at] tonoplast channel protein

Description C35A5.1 /REP_DB=WormBase Gene ID /WP=CE05359 /TR=Q18469 /GB=CAA94903.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=tonoplast channel protein
Cluster 6grp_total > 13      4grp_total > 21      6grp_cgc > 30      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 30
(3)
A 		4.3
(-2.7)
A 		2.9
(-3)
A 		10.8
(-1.3)
A 		27
B 34.6
(0.1)
A 		35.3
(0.2)
A 		3.6
(-3)
A 		62.9
(3)
A 		59
C 36.2
(0.8)
A 		9.9
(-3)
A 		12.7
(-2.6)
A 		51.6
(3)
A 		42
avr 33.6
(1.6) 		16.5
(-1.3) 		6.4
(-3) 		41.8
(3) 		35

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 67.2
(2.8)
A 		54.9
(1.6)
A 		69.6
(3)
A 		39.9
(0.2)
A 		5.4
(-3)
A 		27.2
(-1)
A 		64
B 47.6
(-0)
A 		94.1
(3)
P 		31.2
(-1.1)
A 		37.3
(-0.7)
A 		35.3
(-0.9)
A 		2.5
(-3)
A 		92
C 81.7
(3)
A 		51.5
(0.5)
A 		38.7
(-0.5)
A 		78.2
(2.7)
A 		15.5
(-2.5)
A 		8.9
(-3)
A 		73
avr 65.5
(2.9) 		66.8
(3) 		46.5
(0.7) 		51.8
(1.3) 		18.7
(-2.4) 		12.9
(-3) 		54
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 10.9
(3)
A 		0.9
(-2.9)
A 		0.8
(-3)
A 		1.6
(-2.5)
A 		10
B 11
(1.9)
A 		8.7
(0.8)
A 		0.8
(-3)
A 		13.4
(3)
A 		13
C 12
(3)
A 		2.8
(-3)
A 		4.4
(-2)
A 		8.7
(0.8)
A 		9
avr 11.3
(3) 		4.1
(-1.6) 		2
(-3) 		7.9
(0.8) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 30
1 2 3 4 5 6 range
A 12.4
(0.6)
A 		19.5
(3)
A 		15.2
(1.6)
A 		10.1
(-0.2)
A 		1.6
(-3)
A 		6.6
(-1.3)
A 		18
B 16.6
(0.1)
A 		31.3
(3)
P 		7.6
(-1.7)
A 		9.9
(-1.2)
A 		14.3
(-0.4)
A 		0.9
(-3)
A 		30
C 30.3
(3)
A 		15.7
(-0.2)
A 		9.9
(-1.5)
A 		15.7
(-0.2)
A 		5
(-2.6)
A 		3
(-3)
A 		27
avr 19.8
(2.2) 		22.2
(3) 		10.9
(-0.6) 		11.9
(-0.3) 		7
(-1.9) 		3.5
(-3) 		19
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190939_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12237
Sequence Type Exemplar sequence
Representative Public ID C35A5.1 NCBI
Target Description C35A5.1 /REP_DB=WormBase Gene ID /WP=CE05359 /TR=Q18469 /GB=CAA94903.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=tonoplast channel protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073290(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073290 NCBI Caenorhabditis elegans AQuaPorin or aquaglyceroporin related family member (aqp-5) (aqp-5) mRNA, complete cds. 11/11 None
SNAP00000025020 ENSEMBL cdna:SNAP chromosome:CEL140:V:10494693:10495790:1 11/11 None
GENEFINDER00000025048 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:10494364:10495790:1 11/11 None
C35A5.1 ENSEMBL cdna:known chromosome:CEL140:V:10494693:10495790:1 gene:C35A5.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10494692-10495790(+) 100.0 100.0
Public Domain and Genome References
Gene Title tonoplast channel protein
Gene Symbol aqp-5
Ensembl C35A5.1
Entrez Gene 183221 Entrez gene
SwissProt Q18469 EMBL-EBI
WormBase CE05359 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94903 Hypothetical protein C35A5.1 [Caenorhabditis elegans] ref|NP_505691.1| AQuaPorin or aquaglyceroporin related family member (aqp-5) [Caenorhabditis elegans] 1.0E-160
blast CAE64805 Hypothetical protein CBG09598 [Caenorhabditis briggsae] 1.0E-147
blast CAE64805 Hypothetical protein CBG09598 [Caenorhabditis briggsae] 1.0E-135
blast CAA94779 Hypothetical protein F40F9.9 [Caenorhabditis elegans] emb|CAA94770.2| Hypothetical protein F40F9.9 [Caenorhabditis elegans] ref|NP_505512.3| AQuaPorin or aquaglyceroporin related family member (aqp-4) [Caenorhabditis elegans] 7.0E-50
Protein Domains
Database ID Description E-Value
Pfam IPR000425 EMBL-EBI Major intrinsic protein 1.2E-11
Pfam IPR000425 EMBL-EBI Major intrinsic protein 9.8E-11
Pfam IPR000425 EMBL-EBI Major intrinsic protein 1.2E-11
Pfam IPR000425 EMBL-EBI Major intrinsic protein 9.8E-11
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505691.1 6 21-38,53-75,96-115,180-202,209-231,261-283
C35A5.1 6 21-38,53-75,96-115,180-202,209-231,261-283
SNAP00000025020 6 21-38,53-75,96-115,180-202,209-231,261-283
GENEFINDER00000025048 6 42-64,79-101,122-141,206-228,235-257,287-309
Sequence
Target Sequence 
>C. ELEGANS:190939_AT
attacatccatttgaacttccattctacatgttctctcaatttttcggaggattcgctgg
aaatcttctgagtgcatgtttgcagagaaagagagattttctgaattgggaagactattc
cagcattcgatatcctcttccaacagcaagtattgagtatggatatgataaagttcacaa
ttctacccttgaaaaaactattttgttgacaacacaactagctgcaacaacatcaggaat
aactcatcttggagaaaatcatgaatggtgggaaggattaataagtgaaacaatcacaac
ttatttctttgttacggttattctgatgaatgtagtaaacaatgaaccgagtgaagctac
tccttttatcattggaatgatggttatcgttaacatttttgcaactgcttcaataactgg
tacagctatgaatccagttcgtgctctgtctccaaacatcgtgggtgaaattgttctctc
ttcttcctcactaccaccaaacttctggacataccactacatatattgggcaggacccta
ccttggctctaccattgctgtta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTACATCCATTTGAACTTCCATTC 148 13 276 Antisense
CTACATGTTCTCTCAATTTTTCGGA 128 227 300 Antisense
GAAATCTTCTGAGTGCATGTTTGCA 522 361 335 Antisense
ATATCCTCTTCCAACAGCAAGTATT 285 21 405 Antisense
GATAAAGTTCACAATTCTACCCTTG 400 423 442 Antisense
GTGAAGCTACTCCTTTTATCATTGG 408 481 626 Antisense
GCTATGAATCCAGTTCGTGCTCTGT 239 303 700 Antisense
CGTGGGTGAAATTGTTCTCTCTTCT 341 243 735 Antisense
CTCACTACCACCAAACTTCTGGACA 310 227 762 Antisense
TATATTGGGCAGGACCCTACCTTGG 51 587 797 Antisense
TACCTTGGCTCTACCATTGCTGTTA 567 649 814 Antisense
Sequence Source Affymetrix Proprietary Database