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[190928_s_at] ATP/GTP-binding protein /// RAS-related protein /// ---

Description C47C12.4 /REP_DB=WormBase Gene ID /WP=CE08797 /TR=Q94180 /GB=AAB07572.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=ATP-GTP-binding protein
Cluster 6grp_highest_10_10 > 94      6grp_high1_10_10 > 58      4grp_highest_8_8 > 26      4grp_high_8_8 > 59      6grp_highest_nonorm > 72      6grp_total > 38      4grp_total > 60      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 234
1 2 3 4 range
A 547.9
(0.8)
P 		473
(-1.8)
P 		610
(3)
P 		439.3
(-3)
P 		171
B 545.4
(0.6)
P 		429.6
(-3)
P 		622.3
(3)
P 		483.5
(-1.3)
P 		193
C 518.7
(0.8)
P 		592.7
(3)
P 		575.9
(2.5)
P 		388.1
(-3)
P 		205
avr 537.3
(0.6) 		498.4
(-0.8) 		602.7
(3) 		437
(-3) 		166

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 213
1 2 3 4 5 6 range
A 402.6
(-0.8)
P 		481.5
(3)
P 		399.8
(-0.9)
P 		375.7
(-2.1)
P 		357
(-3)
P 		432.9
(0.7)
P 		125
B 473.7
(1.5)
P 		467.3
(1.3)
P 		433.2
(0.1)
P 		340.4
(-3)
P 		410.3
(-0.6)
P 		517.4
(3)
P 		177
C 502.1
(3)
P 		409.8
(0.2)
P 		360.5
(-1.3)
P 		304.3
(-3)
P 		451
(1.4)
P 		497.3
(2.9)
P 		198
avr 459.5
(2) 		452.9
(1.8) 		397.8
(-0.6) 		340.1
(-3) 		406.1
(-0.2) 		482.5
(3) 		142
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 136
1 2 3 4 range
A 199.5
(3)
P 		102.7
(-1.3)
P 		159.5
(1.2)
P 		64.3
(-3)
P 		135
B 173.9
(3)
P 		106.1
(-2.7)
P 		144.2
(0.5)
P 		103
(-3)
P 		71
C 171.7
(1.7)
P 		170.4
(1.7)
P 		200.7
(3)
P 		65.4
(-3)
P 		135
avr 181.7
(3) 		126.4
(-0.2) 		168.1
(2.2) 		77.6
(-3) 		104

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 132
1 2 3 4 5 6 range
A 74.2
(-3)
P 		171.3
(3)
P 		87.1
(-2.2)
P 		95.3
(-1.7)
P 		107.7
(-0.9)
P 		105.4
(-1.1)
P 		97
B 164.8
(1.4)
P 		155.6
(0.8)
P 		105.2
(-2.1)
P 		90.2
(-3)
P 		166.7
(1.5)
P 		192.9
(3)
P 		103
C 186.1
(3)
P 		125.1
(0.1)
P 		91.7
(-1.5)
P 		61.2
(-3)
P 		145.9
(1.1)
P 		166.5
(2.1)
P 		125
avr 141.7
(1.9) 		150.7
(2.7) 		94.7
(-2) 		82.2
(-3) 		140.1
(1.8) 		154.9
(3) 		73
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 190928_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16576
Sequence Type Exemplar sequence
Representative Public ID C47C12.4 NCBI
Target Description C47C12.4 /REP_DB=WormBase Gene ID /WP=CE08797 /TR=Q94180 /GB=AAB07572.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=ATP-GTP-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076909(11), NM_058778(10), NM_068219(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076909 NCBI Caenorhabditis elegans C47C12.4 (C47C12.4) mRNA, complete cds. 11/11 A 
NM_058778 NCBI Caenorhabditis elegans Y47G6A.27 (Y47G6A.27) mRNA, complete cds. 10/11 None
NM_068219 NCBI Caenorhabditis elegans K08F11.5 (K08F11.5) mRNA, complete cds. 11/11 A 
GENEFINDER00000034074 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3555064:3557402:1 10/11 None
SNAP00000033990 ENSEMBL cdna:SNAP chromosome:CEL140:I:3555583:3557402:1 10/11 None
SNAP00000001909 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4710899:4715797:-1 11/11 None
GENEFINDER00000001917 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4713014:4715797:-1 11/11 None
SNAP00000037134 ENSEMBL cdna:SNAP chromosome:CEL140:X:7756640:7758093:-1 11/11 None
GENEFINDER00000037140 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:7756640:7758017:-1 11/11 None
Y47G6A.27 ENSEMBL cdna:known chromosome:CEL140:I:3555583:3557402:1 gene:Y47G6A.27 10/11 None
K08F11.5.2 ENSEMBL cdna:known chromosome:CEL140:IV:4712833:4715800:-1 gene:K08F11.5 11/11 None
K08F11.5.1 ENSEMBL cdna:known chromosome:CEL140:IV:4712833:4715798:-1 gene:K08F11.5 11/11 None
C47C12.4 ENSEMBL cdna:known chromosome:CEL140:X:7756640:7758093:-1 gene:C47C12.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3555711-3557402(+) 98.83 98.83
chrX:7756638-7758092(-) 100.0 100.0
chrIV:4713041-4715574(-) 95.82 95.82
Public Domain and Genome References
Gene Title ATP/GTP-binding protein
RAS-related protein

Ensembl Y47G6A.27
K08F11.5
C47C12.4
Entrez Gene 177238 Entrez gene
183540 Entrez gene
189998 Entrez gene
SwissProt Q94180 EMBL-EBI
Q94263 EMBL-EBI
Q9BL82 EMBL-EBI
WormBase CE08797 Wormbase
CE11958 Wormbase
CE27262 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:251158_AT GTP-binding protein-related at
CANINE:1605894_S_AT similar to mitochondrial Rho 1 cfa
CANINE:1603893_AT similar to mitochondrial Rho 1 cfa
CANINE:1591194_S_AT similar to mitochondrial Rho 1 cfa
CANINE_2:CFA.20637.1.A1_S_AT similar to mitochondrial Rho 1 cfa
CANINE_2:CFA.577.1.A1_AT similar to mitochondrial Rho 1 cfa
CANINE_2:CFAAFFX.28233.1.S1_S_AT similar to mitochondrial Rho 1 cfa
DROSOPHILA_2:1623553_A_AT Mitochondrial Rho dm
DROSGENOME1:150478_AT Mitochondrial Rho dm
CHICKEN:GGAAFFX.11614.1.S1_S_AT ras homolog gene family, member T1 gga
HG-U133_PLUS_2:224044_AT ras homolog gene family, member T1 hs
HG-U133B:224044_AT ras homolog gene family, member T1 hs
HG-U133_PLUS_2:222148_S_AT ras homolog gene family, member T1 hs
HG-U133A_2:222148_S_AT ras homolog gene family, member T1 hs
HG-U133A:222148_S_AT ras homolog gene family, member T1 hs
HG-U133_PLUS_2:218323_AT ras homolog gene family, member T1 hs
HG-U133A:218323_AT ras homolog gene family, member T1 hs
HG-U133A_2:218323_AT ras homolog gene family, member T1 hs
U133_X3P:G8922837_3P_AT ras homolog gene family, member T1 hs
U133_X3P:G12053352_3P_AT ras homolog gene family, member T1 hs
U133_X3P:HS.14202.2.S1_3P_A_AT ras homolog gene family, member T1 hs
HG-U95E:91428_AT ras homolog gene family, member T1 hs
HG-U95B:46345_AT Ras homolog gene family, member T1 hs
HG-U95B:50998_AT Ras homolog gene family, member T1 hs
HG-U95E:88314_R_AT Ras homolog gene family, member T1 hs
HU35KSUBC:RC_H86769_AT Ras homolog gene family, member T1 hs
HU35KSUBB:RC_H81937_AT Ras homolog gene family, member T1 hs
MU11KSUBA:AA674723_AT ras homolog gene family, member T1 mm
MG-U74AV2:95438_AT ras homolog gene family, member T1 mm
MOE430A:1423533_A_AT ras homolog gene family, member T1 mm
MOUSE430A_2:1423533_A_AT ras homolog gene family, member T1 mm
MOUSE430_2:1423533_A_AT ras homolog gene family, member T1 mm
MOE430B:1440853_AT ras homolog gene family, member T1 mm
MOUSE430_2:1440853_AT ras homolog gene family, member T1 mm
MOE430B:1441758_AT ras homolog gene family, member T1 mm
MOUSE430_2:1441758_AT ras homolog gene family, member T1 mm
MG-U74BV2:105714_AT Ras homolog gene family, member T1, mRNA (cDNA clone IMAGE:3601117) mm
MU19KSUBC:TC36921_AT Ras homolog gene family, member T1, mRNA (cDNA clone IMAGE:3601117) mm
RG-U34C:RC_AI228628_AT ras homolog gene family, member T1 (predicted) rn
RAE230A:1372731_AT ras homolog gene family, member T1 (predicted) rn
RAT230_2:1372731_AT ras homolog gene family, member T1 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7264 small GTPase mediated signal transduction inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5509 calcium ion binding inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB07572 Hypothetical protein C47C12.4 [Caenorhabditis elegans] ref|NP_509310.1| C47C12.4 [Caenorhabditis elegans] 0.0
blast NP_491179 Y47G6A.27 [Caenorhabditis elegans] gb|AAK29889.1| Hypothetical protein Y47G6A.27 [Caenorhabditis elegans] 0.0
blast AAB09163 Hypothetical protein K08F11.5 [Caenorhabditis elegans] ref|NP_500620.1| K08F11.5 [Caenorhabditis elegans] 0.0
blast CAE58574 Hypothetical protein CBG01740 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 0.0014
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 8.5E-5
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 8.9E-9
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 6.3E-5
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 8.9E-9
Pfam IPR002305 EMBL-EBI Aminoacyl-tRNA synthetase, class Ib 1.2E-107
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 6.3E-5
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 1.2E-8
Pfam IPR002048 EMBL-EBI Calcium-binding EF-hand 6.3E-5
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 1.2E-8
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500620.1 1 603-622
K08F11.5.2 1 603-622
K08F11.5.1 1 603-622
GENEFINDER00000001917 1 603-622
Sequence
Target Sequence 
>C. ELEGANS:190928_S_AT
aagtcttccaatgcctggtagtcggagccaaggacgctggaaaaacagtcttcacgcaat
ccctagcaggacgtggaatggctgatgtagcacaaataggacgtcgtcattctccattcg
ttatcaatcgtgtccgagtgaaagaggagagcaagtacctgcttctgcgtgaagttgatg
ttctctcaccacaagatgcgctggggtccggcgagacgtcggcggacgtcgtcgctttcc
tctatgatgtctcgaatcccgattcattcgcgttctgcgccacagtttatcagaaatatt
tctatagaacaaaaacgccgtgtgtcatgattgccacaaaagttgaacgggaagaagttg
accagcgttgggaagtgccgccgaaggaattctgtagacaatttgagctcccgaaaccaa
tcaagttctcgacgggcaatattggtcaatcctcttcaccgatcttcgagcagctcgcca
tgatggcagtttatccacatctccgtcgtgttttctacctcaacgactcgaatcttctct
ccaaaatcacattcggcgctgccatagtagctcta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGTCTTCCAATGCCTGGTAGTCGG 142 157 602 Antisense
GGTAGTCGGAGCCAAGGACGCTGGA 252 505 618 Antisense
GCAATCCCTAGCAGGACGTGGAATG 412 321 657 Antisense
AATAGGACGTCGTCATTCTCCATTC 435 173 696 Antisense
CTCCATTCGTTATCAATCGTGTCCG 471 235 713 Antisense
GCGAGACGTCGGCGGACGTCGTCGC 229 287 812 Antisense
TTCGAGCAGCTCGCCATGATGGCAG 187 695 1066 Antisense
TGATGGCAGTTTATCCACATCTCCG 390 567 1082 Antisense
TTTTCTACCTCAACGACTCGAATCT 594 675 1112 Antisense
CGAATCTTCTCTCCAAAATCACATT 109 255 1130 Antisense
ATTCGGCGCTGCCATAGTAGCTCTA 223 11 1152 Antisense
Sequence Source Affymetrix Proprietary Database