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[190923_at] carboxylate reductase

Description F55G1.9 /REP_DB=WormBase Gene ID /WP=CE07286 /TR=SW:Q20848 /GB=AAB00645.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=carboxylate reductase
Cluster 6grp_highest_10_10 > 78      6grp_high1_10_10 > 88      4grp_highest_8_8 > 18      4grp_high_8_8 > 62      6grp_highest_nonorm > 92      6grp_total > 88      4grp_total > 59      6grp_highest_sub > In      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 365
1 2 3 4 range
A 457.4
(0.4)
P 		529.8
(1.6)
P 		615
(3)
P 		250.3
(-3)
P 		365
B 400.8
(-0.6)
P 		450.9
(0.4)
P 		578.4
(3)
P 		284.9
(-3)
P 		294
C 400.1
(0.5)
P 		394.7
(0.3)
P 		465.9
(3)
P 		306.8
(-3)
P 		159
avr 419.4
(0.1) 		458.5
(0.9) 		553.1
(3) 		280.7
(-3) 		272

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 387
1 2 3 4 5 6 range
A 188.8
(-3)
P 		314.5
(-0.9)
P 		286.6
(-1.4)
P 		437.2
(1.1)
P 		358.2
(-0.2)
P 		549.3
(3)
P 		361
B 261.3
(-3)
P 		276.9
(-2.5)
P 		301.4
(-1.7)
P 		262.2
(-3)
P 		423.1
(2.2)
P 		449.3
(3)
P 		188
C 395.6
(-0.2)
P 		274.3
(-2.3)
P 		344.8
(-1.1)
P 		236.1
(-3)
P 		499.1
(1.6)
P 		576.1
(3)
P 		340
avr 281.9
(-3) 		288.6
(-2.8) 		310.9
(-2.3) 		311.8
(-2.3) 		426.8
(0.6) 		524.9
(3) 		243
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 130
1 2 3 4 range
A 166.5
(3)
P 		115
(0.6)
P 		160.8
(2.7)
P 		36.6
(-3)
P 		130
B 127.8
(2.5)
P 		111.4
(1.2)
P 		134
(3)
P 		60.7
(-3)
P 		73
C 132.4
(1.4)
P 		113.5
(0.3)
P 		162.4
(3)
P 		51.7
(-3)
P 		111
avr 142.2
(2.4) 		113.3
(0.7) 		152.4
(3) 		49.7
(-3) 		103

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 158
1 2 3 4 5 6 range
A 34.8
(-3)
P 		111.8
(1.7)
P 		62.4
(-1.3)
P 		110.9
(1.6)
P 		108.1
(1.4)
P 		133.7
(3)
P 		99
B 90.9
(-1.7)
P 		92.2
(-1.7)
P 		73.2
(-2.8)
P 		69.4
(-3)
P 		171.9
(3)
P 		167.5
(2.7)
P 		103
C 146.6
(1.1)
P 		83.7
(-1.5)
P 		87.8
(-1.3)
P 		47.5
(-3)
P 		161.5
(1.7)
P 		192.8
(3)
P 		145
avr 90.8
(-1.9) 		95.9
(-1.6) 		74.5
(-3) 		75.9
(-2.9) 		147.2
(1.8) 		164.7
(3) 		90
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190923_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9715
Sequence Type Exemplar sequence
Representative Public ID F55G1.9 NCBI
Target Description F55G1.9 /REP_DB=WormBase Gene ID /WP=CE07286 /TR=SW:Q20848 /GB=AAB00645.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=carboxylate reductase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068798(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068798 NCBI Caenorhabditis elegans F55G1.9 (F55G1.9) mRNA, complete cds. 11/11 None
SNAP00000005169 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7476248:7477273:-1 11/11 None
GENEFINDER00000005185 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7476248:7477273:-1 11/11 None
F55G1.9 ENSEMBL cdna:known chromosome:CEL140:IV:7476248:7477283:-1 gene:F55G1.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7476253-7477279(-) 100.0 100.0
Public Domain and Genome References
Gene Title carboxylate reductase
Ensembl F55G1.9
Entrez Gene 177519 Entrez gene
SwissProt Q20848 EMBL-EBI
WormBase CE07286 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6561 proline biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4735 pyrroline-5-carboxylate reductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB00645 Hypothetical protein F55G1.9 [Caenorhabditis elegans] ref|NP_501199.1| F55G1.9 [Caenorhabditis elegans] sp|Q20848|P5CR_CAEEL Putative pyrroline-5-carboxylate reductase (P5CR) (P5C reductase) 1.0E-167
blast CAE61890 Hypothetical protein CBG05881 [Caenorhabditis briggsae] 1.0E-133
blast CAA09332 pyrroline-5-carboxylate reductase [Clostridium sticklandii] 4.0E-40
Protein Domains
Database ID Description E-Value
Pfam IPR000304 EMBL-EBI Delta 1-pyrroline-5-carboxylate reductase 3.9E-119
Sequence
Target Sequence 
>C. ELEGANS:190923_AT
gtgtcaagctggcctgtaaactcaagaccagaatttattatttctgtaatggctggagtt
ccattaaaagttctcaacgcgaagttgccattcgtttcgggaaacactaccattgttcgt
ttgatgccaaatgtagcaagttctattggagccggcgcctcgacgatgtgctatgaaaag
aatgaaaaaattatgaatcaagattctcacatcgaattggcacgcgagttcgccgagtgt
gttggaactgtcgaacttatcccagaaagatgctttaacccggcaatggctattggaggg
agtagtcctgcatggacatttatgtacatcgaatcactggcagatggagccgtcgcacaa
gggctcggacgagcagaggctaaaagattagctgctcaagcagtactcggagcagctcaa
atggtgctcaattcgaactctggatttgatattgaaactcagcactttggatccctcaaa
gatatggtttgctcccctggcggtacaacgattgaaggtgttcgggcacttgagaagaac
ggattccgttacgctgttatgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGTCAAGCTGGCCTGTAAACTCAA 525 483 292 Antisense
ACGCGAAGTTGCCATTCGTTTCGGG 333 91 368 Antisense
ACACTACCATTGTTCGTTTGATGCC 709 107 395 Antisense
TAGCAAGTTCTATTGGAGCCGGCGC 109 651 425 Antisense
GGCGCCTCGACGATGTGCTATGAAA 335 543 445 Antisense
GCACGCGAGTTCGCCGAGTGTGTTG 112 315 511 Antisense
GATGCTTTAACCCGGCAATGGCTAT 103 413 560 Antisense
CACTGGCAGATGGAGCCGTCGCACA 187 199 626 Antisense
GGTGCTCAATTCGAACTCTGGATTT 485 501 714 Antisense
TTGAAACTCAGCACTTTGGATCCCT 663 701 743 Antisense
AACGGATTCCGTTACGCTGTTATGG 339 71 829 Antisense
Sequence Source Affymetrix Proprietary Database