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[190921_at] 7TM chemoreceptor, sri family

Description C05E4.4 /REP_DB=WormBase Gene ID /WP=CE25758 /GEN=sri-4 /TR=O17360 /GB=AAB71273.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster 6grp_total > 90      4grp_total > 21      6grp_cgc > 95      6grp_cgc_sub > 04      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 30.1
(-3)
A 		91.3
(3)
A 		43.2
(-1.7)
A 		70.2
(0.9)
A 		61
B 76.7
(3)
A 		26.5
(-3)
A 		37.5
(-1.7)
A 		57.8
(0.7)
P 		50
C 61.9
(1.3)
A 		35
(-1.9)
A 		25.8
(-3)
A 		76.2
(3)
A 		50
avr 56.2
(0.8) 		50.9
(-0.2) 		35.5
(-3) 		68.1
(3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 14.3
(-1.4)
A 		21.8
(0.9)
A 		25.6
(2)
A 		26.3
(2.2)
A 		9
(-3)
A 		28.9
(3)
A 		20
B 64.5
(3)
A 		41.1
(0.6)
A 		7.1
(-3)
A 		41.4
(0.6)
A 		10.4
(-2.7)
A 		24.1
(-1.2)
A 		57
C 35.6
(-0.6)
A 		29.3
(-1.2)
A 		9.5
(-3)
A 		75
(3)
A 		20.1
(-2)
A 		37.6
(-0.4)
A 		66
avr 38.1
(1.3) 		30.7
(0.1) 		14.1
(-2.8) 		47.6
(3) 		13.2
(-3) 		30.2
(-0) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 11
(-2.6)
A 		19.8
(3)
A 		11.3
(-2.4)
A 		10.3
(-3)
A 		10
B 24.5
(3)
A 		6.6
(-3)
A 		8.7
(-2.3)
A 		12.3
(-1.1)
P 		18
C 20.5
(3)
A 		10.1
(-2.4)
A 		9
(-3)
A 		12.9
(-1)
A 		12
avr 18.7
(3) 		12.2
(-1.3) 		9.7
(-3) 		11.8
(-1.6) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 2.6
(-3)
A 		7.7
(3)
A 		5.6
(0.5)
A 		6.7
(1.8)
A 		2.7
(-2.9)
A 		7
(2.2)
A 		5
B 22.5
(3)
A 		13.7
(0.5)
A 		1.7
(-3)
A 		11
(-0.3)
A 		4.2
(-2.3)
A 		9
(-0.9)
A 		21
C 13.2
(2.1)
A 		8.9
(0.1)
A 		2.4
(-3)
A 		15.1
(3)
A 		6.5
(-1.1)
A 		12.6
(1.8)
A 		13
avr 12.8
(3) 		10.1
(1.3) 		3.2
(-3) 		10.9
(1.8) 		4.5
(-2.2) 		9.5
(0.9) 		10
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 190921_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15179
Sequence Type Exemplar sequence
Representative Public ID C05E4.4 NCBI
Target Description C05E4.4 /REP_DB=WormBase Gene ID /WP=CE25758 /GEN=sri-4 /TR=O17360 /GB=AAB71273.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=chemoreceptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070912(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070912 NCBI Caenorhabditis elegans Serpentine Receptor, class I family member (sri-4) (sri-4) mRNA, complete cds. 11/11 None
SNAP00000009762 ENSEMBL cdna:SNAP chromosome:CEL140:V:748677:752251:1 11/11 None
GENEFINDER00000009771 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:743478:750235:1 11/11 None
C05E4.4 ENSEMBL cdna:known chromosome:CEL140:V:748174:750235:1 gene:C05E4.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:748173-750235(+) 100.0 100.0
Public Domain and Genome References
Gene Title 7TM chemoreceptor, sri family
Gene Symbol sri-4
Ensembl C05E4.4
Entrez Gene 191900 Entrez gene
SwissProt O17360 EMBL-EBI
WormBase CE25758 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4930 G-protein coupled receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB71273 Serpentine receptor, class i protein 4 [Caenorhabditis elegans] ref|NP_503313.1| Serpentine Receptor, class I family member (sri-4) [Caenorhabditis elegans] 0.0
blast CAE62275 Hypothetical protein CBG06334 [Caenorhabditis briggsae] 1.0E-160
blast CAE62275 Hypothetical protein CBG06334 [Caenorhabditis briggsae] 1.0E-159
blast AAB71273 Serpentine receptor, class i protein 4 [Caenorhabditis elegans] ref|NP_503313.1| Serpentine Receptor, class I family member (sri-4) [Caenorhabditis elegans] 1.0E-157
blast AAB71275 Seven tm receptor protein 134 [Caenorhabditis elegans] ref|NP_503312.1| Seven TM Receptor family member (str-134) [Caenorhabditis elegans] 1.0E-153
blast CAE62275 Hypothetical protein CBG06334 [Caenorhabditis briggsae] 1.0E-116
blast CAB04119 Hypothetical protein F15H9.2 [Caenorhabditis elegans] ref|NP_493072.1| Serpentine Receptor, class I family member (sri-15) [Caenorhabditis elegans] 6.0E-46
Protein Domains
Database ID Description E-Value
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 3.5E-21
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 1.0E-126
Pfam IPR003002 EMBL-EBI 7TM chemoreceptor, subfamily 1 2.8E-50
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 4.0E-14
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 8.1E-12
Pfam IPR003003 EMBL-EBI 7TM chemoreceptor, subfamily 2 3.5E-21
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503313.1 7 18-40,53-75,100-122,143-165,203-225,257-279,289-311
C05E4.4 7 18-40,53-75,100-122,143-165,203-225,257-279,289-311
SNAP00000009762 5 23-45,66-88,126-148,180-202,212-234
GENEFINDER00000009771 15 5-27,40-62,82-104,128-150,198-220,250-272,287-309,333-355,414-436,449-471,496-518,539-561,599-621,653-675,685-707
Sequence
Target Sequence 
>C. ELEGANS:190921_AT
catctatcatatttttccctgtctcatcgcgttttcgttataccattcggggctttccaa
ggatgaaaagaattattcaatgcacatgaactatccgggctgcatccattctctagatga
tttcacgtttgacttttacgattatcaagttaacccaacatttatcgcattcgggattct
tatatctgtgtactacgtcatggctggtgtgatagggtttggtctggcctggcggaccgg
tcaagtcctaaaccgttatcgttatatcatgtctgcaagaacataccagctccacaaatc
ttcactgatcacgctaacaactcaggtatccggcccaactctagttctagggattcctgt
atttgtggtctatgtaattgttgccagccagatgacgcagcttcatgacctggcggcaac
tgctccattttttatatccgctcactcggcggtgacaacttcctgtctaattatgaccac
cgcgaattacaagaatttgttcactaagaattacgataacgtgagaaaaaagatgaagtg
caagcggacacctacagcgtctgtcatgtca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATCTATCATATTTTTCCCTGTCTC 370 213 456 Antisense
GTCTCATCGCGTTTTCGTTATACCA 505 469 476 Antisense
ATACCATTCGGGGCTTTCCAAGGAT 160 23 495 Antisense
CAATGCACATGAACTATCCGGGCTG 709 181 533 Antisense
TGTGTACTACGTCATGGCTGGTGTG 236 555 642 Antisense
GAACATACCAGCTCCACAAATCTTC 274 351 734 Antisense
CACGCTAACAACTCAGGTATCCGGC 90 195 765 Antisense
CAGATGACGCAGCTTCATGACCTGG 524 205 844 Antisense
ACTGCTCCATTTTTTATATCCGCTC 121 99 874 Antisense
TATCCGCTCACTCGGCGGTGACAAC 163 659 890 Antisense
GACACCTACAGCGTCTGTCATGTCA 23 369 1002 Antisense
Sequence Source Affymetrix Proprietary Database